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Penicillium sp. strain and application thereof

A technology of Penicillium and strains, applied in the application, fungicides, fungi and other directions, can solve the problems of unformed products such as anthracnose.

Inactive Publication Date: 2018-10-16
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Biological control of anthrax is currently not available on the market

Method used

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  • Penicillium sp. strain and application thereof
  • Penicillium sp. strain and application thereof
  • Penicillium sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Strain acquisition:

[0065] The bacterial strain of the present invention is obtained by separation, purification and culture according to the conventional method of air microorganism separation, and the medium used for separation is tiger red medium, which is identified as Penicillium sp. by fungal ITS and named YAnti45-1. figure 1 The blast gene comparison results of YAnti45-1 Penicillium strain on Genbank, figure 2 It is the NJ phylogenetic tree of the YAnti45-1 Penicillium strain. It can be seen from the figure that the YAnti45-1 strain belongs to the Penicillium genus, and there are obvious differences between the YAnti45-1 Penicillium strain and other strains.

[0066] Morphology: On the CMD medium, the conidia area presents protrusions of different sizes, and the aerial hyphae are not luxuriant. On the PDA medium, the aerial mycelium is luxuriant, and the sporulation area spreads all over the entire plate, dark green to gray green, and produces yellow pigment ...

Embodiment 2

[0072] 1. Cultivate the YAnti45-1 strain on a PDA plate, and take it out for later use when spores grow on the plate;

[0073] 2. Collect the spores on the plate under sterile conditions, insert the spores into the PDA liquid medium containing glass beads, and culture at 27°C 120rpm / min;

[0074] 3. Cultivate for 2 days, and wait until the medium is covered with tiny mycelium balls, this is the Penicillium seed solution;

[0075] 4. Aseptically insert the grown penicillium seed liquid into the pre-sterilized liquid fermentation medium (10 grams of cold-pressed bean cake powder, 10 grams of fructose), and the amount of insertion is 1% of the liquid fermentation medium loading % calculation, cultured at 26°C for 4 days, dissolved oxygen was 71% (rotating speed was 158rpm / min) in 0 to 36 hours, and cultivated after 36 hours at 60% dissolved oxygen (rotating speed was 133rpm / min);

[0076] 5. The antagonism ability of the YAnti45-1 strain culture to anthrax bacteria was measured by...

Embodiment 3

[0078] 1. Cultivate the YAnti45-1 strain on a PDA plate, and take it out for later use when spores grow on the plate;

[0079] 2. Collect the spores on the plate under sterile conditions, insert the spores into the PDA liquid culture containing glass beads, and cultivate at 30°C and 130rpm / min;

[0080] 3. Cultivate for 2 days, and wait until the medium is covered with tiny mycelium balls, this is the Penicillium seed solution;

[0081] 4. Aseptically insert the long-growing penicillium seed liquid into the pre-sterilized liquid fermentation medium (wherein 20 grams of cold-pressed bean cake powder, 20 grams of fructose), the amount of insertion is 2% of the liquid volume of the new medium % calculation, cultured at 28°C for 6 days, 0 to 36 hours dissolved oxygen was 75% (rotating speed was 168rpm / min), and after 36 hours dissolved oxygen was 70% (rotating speed was 156rpm / min) for cultivation;

[0082] 5. The antagonism ability of YAnti45-1 strain culture to anthrax bacteria...

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Abstract

The invention relates to a penicillium sp. strain and an application thereof and belongs to the technical field of microbe applications. The provided penicillium sp. strain has the collection number of CGMCC No.14142. The provided penicillium sp. strain can have an antagonistic action for colletotrichum, a culture of the penicillium sp. strain has an antagonistic action for colletotrichum throughliquid culture, and the antagonistic rate of colletotrichum is 70%-90%.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to a Penicillium strain capable of antagonizing anthrax and its application. Background technique [0002] Plant disease is the enemy of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), 10% of grain production is lost due to disease, and 12% of cotton production is due to disease. half of China's total agricultural output value. The occurrence of plant diseases not only directly affects the income of farmers in our country, but also affects the development of other related industries, especially the agricultural product processing industry. In 1880, the grape planting industry in the Bordeaux region of France was on the verge of bankruptcy due to downy mildew. The most serious thing is that it is not conducive to the long-term and sustainable development of agriculture and its related indus...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/80
CPCA01N63/30C12N1/14C12N1/145C12R2001/80
Inventor 乔敏余泽芬丰波田伟光边照辉
Owner YUNNAN UNIV
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