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Halobacillus sp. strain and use thereof

A technology of bacillus and bacterial liquid, which is applied in the field of plant disease control and microorganisms, can solve the problems of long treatment cycle, easy to cause pollution, cure the symptoms but not the root cause, and achieve the effects of reducing the incidence rate, increasing the yield, and broad application prospects

Pending Publication Date: 2021-11-30
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical control mainly replaces the planting plots every year and avoids repeated planting to prevent the occurrence of ginger root diseases, so there is a shortcoming of long treatment period
Chemical control mainly uses a variety of pesticides to prevent and control pathogenic bacteria. This method is easy to cause pollution and treats the symptoms but not the root cause. It is easy to cause a vicious cycle of disease occurrence and deterioration of soil physical and chemical properties.

Method used

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  • Halobacillus sp. strain and use thereof
  • Halobacillus sp. strain and use thereof
  • Halobacillus sp. strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Isolation and purification method of Bacillus halo Y10312

[0060] The separation and purification method of the halobacterium bacillus Y10312 bacterial strain of the present invention, the steps are as follows:

[0061] S1: Weigh 10g of soil sample, said soil sample comes from the mangrove soil of Hailing Island Mangrove National Wetland Park in Guangdong Province; then, add 90ml of sterile water to the soil sample in the ultra-clean workbench, place the shaker Shake on the mixer for 60 minutes, so that the soil sample is evenly dispersed in the diluent to form a soil suspension; after the soil is dispersed, draw 100ul of the soil suspension into 900ul of sterile water to obtain a 10-fold dilution, and then dilute 10 times in turn to obtain a 10-fold dilution. 2 Double dilution, 10 3 Double dilution, 10 4 Double dilution, 10 5 double dilution and 10 6 Double dilution, the whole process is carried out in the ultra-clean workbench.

[0062] S2: Take 100ul...

Embodiment 2

[0067] Embodiment two: the antagonism of bacillus halo Y10312 to Pythium ingross and Fusarium solani

[0068] S1 Inoculation: Take the glycerin seed solution stored at -20°C in Example 1 and inoculate it into 2216E liquid medium with an inoculum volume ratio of 1:100 between the bacterial solution and the medium, and place it in a constant temperature shaker for constant temperature shaking at 28°C. 28h, to obtain the activated bacterial liquid;

[0069] Identification of S2 medium: use plate confrontation method. Get the pathogenic flora Pythium insolata ( image 3 ) and Fusarium solani ( Figure 4 ) bacterial block (diameter 0.8cm), placed in the center of the PDA solid medium, at a distance of 2cm from the bacterial block, absorb 20ul of activated bacterial liquid and spot in three parallel groups; at the same time, the plate of the control group is only spot in the center of the plate Pathogens: After inoculation, place in a biochemical incubator at 28°C for 3-5 days to...

Embodiment 3

[0071] Embodiment three: Bacillus halo Y10312 culture method

[0072] This example provides the cultivation methods of two strains of Bacillus halobacterium Y10312.

[0073] The first culture method uses 2216E liquid medium, the process is as follows:

[0074]Activation before cultivation: Take the glycerin seed liquid stored at -20°C in Example 1 and inoculate it into 2216E liquid medium with an inoculum volume ratio of 1:100 between the bacterial liquid and the medium, and place it in a constant temperature shaker at 28°C for constant temperature oscillation. Cultivate for 28 hours to obtain activated bacterial liquid.

[0075] S1 Inoculation: Take the activated bacterial liquid and inoculate it into 500ml 2216E liquid medium according to the volume ratio of the bacterial liquid to the medium: 1:100;

[0076] S2 culture: place the above-mentioned culture bottle inoculated with the bacillus halobacterium strain Y10312 in a constant temperature shaker, and cultivate it at a ...

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Abstract

The invention relates to a halobacillus sp. strain and use thereof. The halobacillus sp. is deposited in the China Center for Type Culture Collection and has a deposite number of CCTCC NO: M 2020558. The halobacillus Y10312 can effectively antagonize pathogens of plant root rot, especially has an antagonism rate of 79% or more on pythium myriotylum and fungi imperficti, can remarkably reduce morbidity of the plant root rot, greatly improves the crop yield, and thus has a wide use prospect in treating plant diseases.

Description

technical field [0001] The invention relates to the field of plant disease control and microorganism technology, in particular to a strain of Bacillus halobacterium and its application. Background technique [0002] Plant root rot is a disease caused by fungi. Early susceptible plants do not show obvious symptoms. Later, as the degree of root rot intensifies, the function of plants to absorb water and nutrients gradually weakens. The new leaves turn yellow first, and the upper leaves of the plant wilt when the light is strong and the evaporation is large around noon, but they can recover at night. When the disease is severe, the wilting condition cannot be recovered at night, and the leaves of the whole plant turn yellow and wither. At this time, the root bark turns brown and separates from the pith, and finally the whole plant dies. Root rot can be caused by the infection of various pathogens such as Pythium, Fusarium and Phytophthora. The pathogens overwinter in the soil...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A01C1/00A01G13/00A01G22/25C12R1/01
CPCC12N1/20A01N63/20A01C1/00A01G13/00A01G22/25
Inventor 王磊黄雅婷姜峰曾远婷陈妙芳郭燕锋
Owner SOUTH CHINA AGRI UNIV
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