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Bio-control bacterium Kg2A capable of efficiently inhibiting fusarium and bacillus anthraci and applications thereof

A technology of bio-control bacteria and strains, applied in the direction of application, chemicals for biological control, bacteria, etc., can solve the problems of no official report of antagonistic bacteria, no biological pesticides for preventing and controlling pepper diseases, weak technology, etc., and achieve antibacterial Stable ability, strong adaptability, easy to cultivate effect

Active Publication Date: 2015-10-21
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isolation and screening from peppers to obtain biocontrol strains with antagonistic effects on pepper diseases Fusarium wilt and anthracnose is of great significance to the prevention and control of pepper diseases, but the relevant technology is still very weak at present, and no antagonistic bacteria that can effectively control pepper diseases have been found so far. Official reports, and no reports of biopesticides for the control of pepper diseases

Method used

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  • Bio-control bacterium Kg2A capable of efficiently inhibiting fusarium and bacillus anthraci and applications thereof
  • Bio-control bacterium Kg2A capable of efficiently inhibiting fusarium and bacillus anthraci and applications thereof
  • Bio-control bacterium Kg2A capable of efficiently inhibiting fusarium and bacillus anthraci and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation, cultivation, screening and purification of strain Kg2A

[0026] 1. Sampling

[0027] The samples were pepper leaves from Dapo Town, Hainan Province. The collected leaves were put into plastic bags and recorded.

[0028] 1.2 Surface disinfection of materials and separation of antagonistic bacteria

[0029] 1.2.1 Rinse with clean water and cut off diseased leaves

[0030] Wash off the dust and grit on the leaves with clean water and let them dry. Use scissors to cut small leaves with a size of 5 × 5mm at the junction of disease and health for future use.

[0031] 1.2.2 Sample disinfection

[0032] Put the clipped small diseased leaves into a beaker and soak them in mercuric chloride for 30 seconds. During the soaking process, pay attention to constantly stirring the small diseased leaves, then rinse them with sterile water three times, dry them, and set them aside.

[0033] 1.2.3 Bacterial isolation and culture

[0034] Use sterilized tweezers to paste th...

Embodiment 2

[0039] Identification of strain Kg2A

[0040] 1. Microbiological characteristics:

[0041] Observing the morphological characteristics of the biocontrol strains cultivated by streaking in the incubator at 37°C, it was found that the biocontrol strain Kg2A was straight rod-shaped, less chained, with round or square ends; spores were oval, oval, columnar, round Shaped, non-capsulated, belonging to the genus Bacillus. After Gram staining, the bacteria showed blue-purple, which was Gram-positive bacteria.

[0042] 2. Molecular biological identification:

[0043] (1) DNA extraction: Genomic DNA was extracted using a general kit in the art;

[0044](2) PCR reaction: Bacterial universal primers 8F and 1492R, synthesized by Yingwei Jieji (Shanghai) Trading Company, the structure is as follows: 8F 5'-AGAGTTTGATCCTGGCTCAG-3'

[0045] 1492R 5'-GGTTACCTTGTTACGACTT-3',

[0046] The 16S rDNA gene amplification results of the biocontrol strain Kg2A are as follows: figure 1 As shown, th...

Embodiment 3

[0114] Bacteriostatic Test of Biocontrol Bacteria Kg2A on Pathogenic Bacteria

[0115] In the center of the pre-prepared PDA plate, inoculate the test pathogenic bacteria block with a diameter of 6mm, and at the same time inoculate the bacteria to be tested at two symmetrical points 25mm away from the center of the PDA plate. After 6 days, the antibacterial status was observed.

[0116]

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Abstract

The invention belongs to the field of microbial pesticide, and specifically relates to a bio-control bacterium Kg2A and applications thereof on controlling pepper wilt and pepper anthracnose. The bio-control bacterium is Bacillus amyloliquefaciens Kg2A, and the preservation number is CGMCC No.10918. The invention also discloses the molecular biological characteristics, physiological-biochemical characteristics, and culture method of the provided bacterium strain, and proves that the provided bio-control bacterium Kg2A has an activity on inhibiting fusarium that may cause pepper wilt and bacillus anthraci that can cause pepper anthracnose.

Description

technical field [0001] The invention belongs to the field of microbial pesticides, and in particular relates to a strain of bacillus amyloliquefaciens Kg2A and its application in preventing and treating pepper wilt and anthracnose. Background technique [0002] Pepper is the main tropical agricultural product in my country. It is an important crop for the majority of farmers in Hainan to generate income. It is also a pillar of the economic development of Hainan Province. It plays an increasingly important role in the development of the national economy. This is because, on the one hand, its seeds contain volatile oil, piperine, crude fat, crude protein, etc., which are people's favorite condiments; Bronchial mucous membrane stimulant, has the effect of expelling cold, invigorating the stomach, and warming the middle. Dysentery, syphilis, cold, rheumatic bone pain, lung congestion, cough and phlegm and many other diseases; in addition, the pepper industry has become an impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 郑肖兰李锐吴伟怀习金根贺春萍易克贤梁艳琼郑金龙秦周
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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