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Liquid fermentation culture medium for colletotrichum spore production

A technology of liquid fermentation and medium, applied to fungi, methods using spores, methods based on microorganisms, etc., can solve the problems of waste of grass resources, non-utilization, waste of weeds, etc., and achieve extremely easy to obtain, large quantities Simple effect of preparation and artificial planting

Active Publication Date: 2019-02-19
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are various types of weeds in my country and abundant biomass resources, but after the initial chemical control, most of the remaining weeds are wasted in the fields, or weeds in non-cultivated land are seriously wasted and basically cannot be used. This is undoubtedly a huge waste for weed populations that contain a large amount of biomass resources

Method used

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  • Liquid fermentation culture medium for colletotrichum spore production
  • Liquid fermentation culture medium for colletotrichum spore production
  • Liquid fermentation culture medium for colletotrichum spore production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 The preparation method of culture medium provided by the present invention

[0019] Use field or artificially grown shepherd's purse as raw material, remove the root of shepherd's purse, wash it, add distilled water, boil for 30-35 minutes, collect and filter the juice obtained; add soybean powder, sucrose and potassium dihydrogen phosphate to the juice; add distilled water, Adjust pH, constant volume; put the solution after constant volume at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization. According to the composition, the medium was named shepherd's purse soybean flour medium (CGS medium).

Embodiment 2

[0020] Example 2 Screening of weed components in culture medium

[0021] The shepherd's purse in Example 1 is replaced by other weeds, and other steps are unchanged to make a culture medium. In the present embodiment, 12 kinds of Artemisia sativa, Dawanhua, Amaranthus retroflexi, Flea scorpion, Pig scorpion, Chinese crabgrass, Setaria, Goosegrass, Mai Niang, Jiejie wheat, brome and wild oats were used at the same time. Weeds were used as weeds, and compared with the medium supplemented with shepherd's purse.

[0022] From the third day onwards, take out 3 bottles of liquid shake cultured bacterial cells every day, draw a certain amount of liquid after shaking evenly, and count the number of conidia on a hemocytometer.

[0023] Table 1 Sporulation of anthracnose bacteria in different weed culture medium

[0024]

[0025] As can be seen from the data provided in Table 1, the 13 most common weeds in the field were added with the same content. According to the method provided...

Embodiment 3

[0026] Embodiment 3 Culture medium provided by the present invention is contrasted with the culture effect of other commonly used fungal culture medium

[0027] Potato dextrose medium (PDA): 200 g of potatoes, manually cut into potato pieces of about 2×2 cm, boiled in water for 30 minutes, filtered through gauze, and retained the juice. Add 20 g of glucose and 20 g of agar to the juice obtained by filtration, and set the volume to 1000 ml. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization.

[0028] Carrot culture medium (CA): 200 g carrots, manually cut into carrot pieces of about 2 × 2 cm, boiled in boiling water for 30 minutes, filtered through gauze, and retained the juice. Add 20 g of agar to the juice obtained by filtration, and adjust the volume to 1000 ml. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 2...

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Abstract

The invention provides an excellent culture medium suitable for liquid fermentation culture of colletotrichum. Every 1,000 ml of the culture medium is prepared from 20-40 g of shepherd's purse, 3-6 gof soybean meal, 2-4 g of saccharose, 0.5 g of monopotassium phosphate and the balance 1,000 ml of distilled water, wherein the pH is adjusted to be 6.5. By means of the culture medium, a large quantity of colletotrichum conidiospores can be quickly and efficiently obtained. According to the excellent culture medium, weeds serve as the main component of the culture medium, the cost for fermentation culture of colletotrichum is reduced, and the culture medium for efficient colletotrichum spore production is obtained.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, and in particular relates to a liquid fermentation medium for sporulation of anthrax bacteria. Background technique [0002] Plant anthracnose is a kind of disease that commonly occurs in crops, fruits and vegetables, and ornamental plants in my country, and is mainly caused by pathogenic bacteria of the genus Anthracnose. The classification of anthracnose fungus is classified into Ascomycota, Faecalimycetes, Hypocrenae suborder Pyrethiaceae, and Pyrethyme genus. The fungus of the genus Anthracnose is widely distributed in my country, from Northeast China to Hainan Province, and a variety of plants can be used as its host, which has a wide range of parasitic properties. The fungus often harms plant leaves, flowers, fruits, stems and twigs, causing anthracnose of various crops, causing symptoms such as leaf spot, leaf withering, flower rot, fruit rot and branch withering, affect...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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