Method for identifying and screening banana panama disease resisting seedlings

A banana wilt and seedling technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of uncontrollable concentration, no disease of plants, high cost, etc., achieve improved efficiency and accuracy, and simple preparation process , the effect of easy access

Inactive Publication Date: 2018-12-18
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] The above-mentioned potted plant inoculation method mainly has the following problems: 1. Use soil or fine sand to carry out potted plant irrigation and root soaking and transplanting. In order to avoid the interaction of soil microorganisms from affecting the accuracy of the test results, large-scale soil disinfection must be carried out in advance. , inconvenient operation, not conducive to mass screening of disease-resistant seedlings; 2. Because banana wilt is a soil-borne disease, the actual production does not use soil or fine sand as the seedling substrate; 3. Hydroponics method for identification of disease-resistant seedlings (CN 102771326A), it is necessary to set up a banana seedling hydroponic system and configure a large amount of nu...

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  • Method for identifying and screening banana panama disease resisting seedlings
  • Method for identifying and screening banana panama disease resisting seedlings
  • Method for identifying and screening banana panama disease resisting seedlings

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Embodiment 1

[0036] 1. Preparation of pathogenic substrate inoculum

[0037] Use the commonly used banana seedling substrate (mainly coconut bran) in the market, add corn flour and water according to a certain mass ratio, that is, coconut bran: corn flour: water = 5:1:1. Mix evenly, divide into glass bottles with a bottom diameter of 6.5 cm and a height of 9.0 cm, cover them, and sterilize twice under high temperature and high pressure at 121°C for 20 minutes. Inoculate the banana fusarium wilt pathogenic bacteria cake cultivated in PDA medium for 5 days under sterile conditions, culture in the dark for 14-21 days in a 28°C incubator, take samples, conduct microscopic examination, and combine the gradient dilution method to determine the concentration of pathogenic bacteria spores in the substrate can reach 10 6 -108 per g, divided into bottles according to the actual dosage, and the rest can continue to be cultivated for 3 months, and the concentration of pathogenic bacteria spores in the...

Embodiment 2

[0054] The present invention is a method for identification and screening of banana fusarium wilt disease-resistant seedlings, for Williams B6 (susceptible variety), Guijiao No. 9 (medium-resistant variety), and GCTCV-119 (high-resistant variety) with three different resistances Identification of disease resistance of banana varieties and comparison with field disease evaluation.

[0055] 1. Prepare pathogenic bacteria. Based on the previous pathogenicity detection test, the pathogenic strain Fusarium oxysporum Cuban specialization No. 4 physiological race (Foc4) with strong pathogenicity was used. Physiological race No. 4 strain was inoculated in sterile potato solid medium (200g of potatoes, 20g of glucose, 1000mL of water, sterilized at 121°C for 20min), and cultivated for 5-7 days at a temperature of 25-28°C under constant temperature conditions. The fungus cake is ready for use.

[0056] 2. Select healthy tissue-cultured banana seedlings with a leaf age of 5-6 leaves an...

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Abstract

The invention provides a method for identifying and screening banana panama disease resisting seedlings. The method comprises the following steps of A, preparing disease cause matrix inoculants, mixing coconut bran, corn flour and water to uniform, distributing in glass cylinders and performing high-temperature autoclaving; B, preparing the disease cause matrix, pouring the matrix from the cylinder, performing rolling crushing, adding the new seedling culturing medium according to a certain mass ratio, mixing to uniform for standby, and maintaining the concentration of the measured matrix pathogen spores to 105-106/g; C, performing banana seedling planting and daily management; and D, performing condition investigation and grading, after banana panama disease pathogens are inoculated, performing disease grade identification on the banana seedlings according to a disease evaluation grade, wherein the identification time of the panama disease resisting kind can be evaluated after three months, recording to the disease incidence rate and condition index of the banana seedlings, and determining the disease resistance of the plant according to the disease incidence rate and the condition index. The disease cause matrix inoculants are prepared through directly adding corn flour into the seedling culturing matrix, thereby saving cost and realizing a simple preparing process.

Description

technical field [0001] The invention belongs to the field of biological control of banana diseases, in particular to a method for identifying and screening banana fusarium wilt-resistant and disease-resistant seedlings. Background technique [0002] Banana Fusarium wilt, also known as Panama disease and yellow leaf disease, is a devastating banana fungal disease caused by Fusarium oxysporum f.sp.cubense (Foc). Soilborne diseases. The disease has a wide transmission route, strong pathogenicity, rapid spread, high infection rate, difficult control, high mortality of infected plants, and is called "banana cancer". Once the banana wilt occurs, it will be extremely destructive and will seriously harm the banana industry. The disease was discovered in Australia in 1874, and it broke out in Panama and several countries in South America in the middle of the last century, with about 43hm 2 The destruction of banana orchards in the United States made the internationally traded bana...

Claims

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Application Information

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IPC IPC(8): A01G7/00A01G22/05A01G31/00A01G7/06
CPCA01G7/00A01G7/06A01G22/05A01G31/00
Inventor 周维黄素梅李朝生田丹丹覃柳燕韦莉萍韦绍龙韦弟张进忠龙盛风何章飞孙嘉曼
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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