Method for identifying and screening banana panama disease resisting seedlings

Abstract

The invention provides a method for identifying and screening banana panama disease resisting seedlings. The method comprises the following steps of A, preparing disease cause matrix inoculants, mixing coconut bran, corn flour and water to uniform, distributing in glass cylinders and performing high-temperature autoclaving; B, preparing the disease cause matrix, pouring the matrix from the cylinder, performing rolling crushing, adding the new seedling culturing medium according to a certain mass ratio, mixing to uniform for standby, and maintaining the concentration of the measured matrix pathogen spores to 105-106 / g; C, performing banana seedling planting and daily management; and D, performing condition investigation and grading, after banana panama disease pathogens are inoculated, performing disease grade identification on the banana seedlings according to a disease evaluation grade, wherein the identification time of the panama disease resisting kind can be evaluated after three months, recording to the disease incidence rate and condition index of the banana seedlings, and determining the disease resistance of the plant according to the disease incidence rate and the condition index. The disease cause matrix inoculants are prepared through directly adding corn flour into the seedling culturing matrix, thereby saving cost and realizing a simple preparing process.

Description

technical field [0001] The invention belongs to the field of biological control of banana diseases, in particular to a method for identifying and screening banana fusarium wilt-resistant and disease-resistant seedlings. Background technique [0002] Banana Fusarium wilt, also known as Panama disease and yellow leaf disease, is a devastating banana fungal disease caused by Fusarium oxysporum f.sp.cubense (Foc). Soilborne diseases. The disease has a wide transmission route, strong pathogenicity, rapid spread, high infection rate, difficult control, high mortality of infected plants, and is called "banana cancer". Once the banana wilt occurs, it will be extremely destructive and will seriously harm the banana industry. The disease was discovered in Australia in 1874, and it broke out in Panama and several countries in South America in the middle of the last century, with about 43hm 2 The destruction of banana orchards in the United States made the internationally traded bana...

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Problems solved by technology

[0005] The above-mentioned potted plant inoculation method mainly has the following problems: 1. Use soil or fine sand to carry out potted plant irrigation and root soaking and transplanting. In order to avoid the interaction of soil microorganisms from affecting the accuracy of the test results, large-scale soil disinfection must be carried out in advance. , inconvenient operation, not conducive to mass screening of disease-resistant seedlings; 2. Because banana wilt is a soil-borne disease, the actual production does not use soil or fine sand as the seedling substrate; 3. Hydroponics method for identification of disease-resistant seedlings (CN 102771326A), it is necessary to set up a banana seedling hydroponic system and configure a large amount of nutrient solution, the cost is high, the hydroponic cultivation can not truly simulate the normal growth environment of the banana root under natural conditions, after the pathogenic bacteria contact the nutrient solution, they multiply in large numbers, and the concentration cannot be controlled. Therefore, it affects the accuracy of the test results

4. Potted root irrigation, root soaking transplanting and covering inoculation of pathogenic bacteria often cause the plants of susceptible banana varieties not to develop disease. The reason may be that under the influence of nutritional conditions and environmental conditions, the distribution of pathogenic bacteria in the seedling medium is uneven, and the inoculation concentration cannot be adjusted. keep stable

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