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Water-purifying bacillus subtilis, bacterial agent and solid fermenting process and use

A technology of Bacillus subtilis and water-purifying inoculants, applied in bacteria, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as flooding of mud and skin in shrimp ponds, destruction of aquaculture water bodies, and low number of effective viable bacteria.

Inactive Publication Date: 2004-06-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, the following problems also exist in the breeding environment of prawns: First, the mud in the shrimp ponds is flooded
Due to the extensive use of chemicals and antibiotics in shrimp ponds, a large number of algae and organisms died, and in shrimp ponds that rarely used beneficial microbial preparations, about 20 days after entering the water and throwing seedlings, a large amount of smelly mud surfaced. Aquaculture water bodies cause great damage
The second is the phenomenon of eutrophication in aquaculture water bodies in some areas
Third, the pH value of the shrimp pond is unstable
There are many problems in the technology and products of these fermentation agents: first, the effective number of viable bacteria in the product is low; Raw materials and production methods make the product more expensive
If the price of imported products is higher, although the effect is more obvious, the cost of use is unacceptable to users, and the cost of application per month is 50 yuan / mu or above
Third, the transportation and storage of liquid products are not very convenient

Method used

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  • Water-purifying bacillus subtilis, bacterial agent and solid fermenting process and use
  • Water-purifying bacillus subtilis, bacterial agent and solid fermenting process and use
  • Water-purifying bacillus subtilis, bacterial agent and solid fermenting process and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Isolation, identification and screening of bacterial strains

[0036] 1. Isolation and screening of bacterial strains:

[0037] 1. Medium for separation: 5.0g beef extract, 10.0g peptone, 5g NaCl, 1000ml water, 20g agar, adjust pH to 7.2-7.4.

[0038] 2. Soil sample separation method - dilution plate counting method (Edited by Li Fudi et al., "Experimental Technology of Agricultural Microbiology", China Agricultural Press, 1996).

[0039] Take fresh soil samples (collected from the paddy soil at the southern end of the campus of Huazhong Agricultural University), dilute with water, take three dilutions of 10-4, 10-5, 10-6 and smear the dishes, cultivate at 28°C for two days, count, and pick the soil A single colony at the highest dilution in the sample was inoculated onto the slant.

[0040] 3. Screening of excellent strains:

[0041] The starting strains for screening were 87 strains of bacteria isolated from paddy soil. Taking the COD removal rate as ...

Embodiment 2

[0047] Example 2: Design of solid high-density fermentation process and production of Bacillus subtilis inoculum for water purification

[0048] Figure 1 shows the flow chart of the solid high-density fermentation process of bacterial agent of the present invention.

[0049] 1. Erlenmeyer flask liquid strain expansion culture: 4 500ml Erlenmeyer flasks, respectively filled with 150ml PDA medium (preparation as mentioned above), inoculated with activated FY99-01 strain after sterilization, placed on a shaker at 30°C (200rpm ) for 36-48hrs.

[0050] 2. canned bottle solid bacterial classification expands cultivation: basic culture medium is rice straw powder 0.6kg and wheat bran 2.4kg, adds bentonite 0.8kg again, ammonium sulfate 0.2kg (in another embodiment, the actual addition of bentonite and ammonium sulfate can be According to the ratio range described above in the present invention), water 8L, after mixing the seasoning, the culture material is divided into 160 canned bot...

Embodiment 3

[0052] Embodiment 3: the application of Bacillus subtilis water purifying bacterial agent of the present invention in prawn culture

[0053] In April 2002, the inventor of the present application selected three high-level ponds with similar size and water quality in Sanya City, Hainan Province, China, and carried out an 11-day experiment to carry out the growth and decline of Bacillus subtilis preparations in the shrimp culture ponds and improve water quality. In the experiment of the effect, the changes of Bacillus subtilis, Vibrio, COD, hydrogen sulfide, nitrite, and total alkalinity in the water after applying the Bacillus subtilis inoculum in the culture pond were tracked, detected and analyzed. and evaluation. The results of the test materials and methods are as follows:

[0054] 1. Materials and methods

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Abstract

The invention relates to a water-quality purifying Bacillus substilis, CCTCC, M202045, and Bacillus substilis preparation. It uses Bacillus substilis to cultivate and ferment in proper C-N ratio in the solid culture medium, and then dries to produce the preparation. The efficiency of the Bacillus substilis to eliminate water body COD is high. The Bacillus substilis can quickly degrade organics, and has a certain denitrifying and residue degrading ability, and stronger sulfide degrading ability. The test result shows that: COD eliminated can reach 76% in 24 hours, and the Bacillus substilis has excellent characters of salt resistance, osmotic pressure resistance, and maintaining the pH value of the breeding water body.

Description

technical field [0001] The invention belongs to the technical field of agricultural application microorganisms. Specifically, it relates to the selection and breeding of Bacillus subtilis bacterial strain for purifying water quality, the water purifying agent produced by the bacterial strain, and the solid fermentation process and application method of the microbial agent. Background technique [0002] The total annual output of aquatic products in my country exceeds 45 million tons, ranking first in the world. However, my country's aquaculture industry is currently facing some serious challenges, such as frequent outbreaks of diseases, serious water pollution, and degradation of fishery resources. These problems seriously restrict the sustainable development of my country's aquaculture industry. [0003] Shrimp farming is one of the leading industries of aquaculture in my country, which has obvious economic and social benefits. However, the following problems also exist i...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20
Inventor 梁运祥葛向阳胡咏梅李学雄龚守成阎淳泰田焕章王绩殷翱陈正军梅余霞
Owner HUAZHONG AGRI UNIV
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