Preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus

An effective part, Panax notoginseng technology, applied in the direction of medical preparations, anti-inflammatory agents, pharmaceutical formulations, etc. containing active ingredients, to achieve the effects of high ingredient content, clear chemical composition, and simple operation

Inactive Publication Date: 2018-04-20
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the anti-inflammatory effect of Panax notoginseng

Method used

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  • Preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus
  • Preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus
  • Preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The preparation method of the anti-inflammatory effective part of Panax notoginseng, the specific steps are as follows:

[0023] Step 1. Crushing and extracting: After crushing Pingbian notoginseng, leaching with methanol for 3 times according to the liquid-to-solid ratio of 1:10kg / L, the combined extracts were concentrated under reduced pressure to obtain 250.6g of methanol extract;

[0024] Step 2, petroleum ether degreasing: disperse the methanol extract obtained in step 1 with 1L of water, and use petroleum ether to extract according to the ratio of 1:1.5 to obtain an aqueous raffinate solution to remove fat-soluble impurities;

[0025] Step 3, n-butanol extraction: the raffinate aqueous solution obtained in step 2 is extracted with saturated n-butanol according to the ratio of 1:2, and the extracted organic solution is concentrated under reduced pressure to obtain n-butanol extract;

[0026] Step 4, separation and purification: the n-butanol extract obtained in ste...

Embodiment 2

[0055] The preparation method of the anti-inflammatory effective part of Panax notoginseng, the specific steps are as follows:

[0056] Step 1. Crushing and extracting: After crushing Pingbian notoginseng, leaching with methanol for 3 times according to the liquid-to-solid ratio of 1:5kg / L, the combined extracts were concentrated under reduced pressure to obtain 250.6g of methanol extract;

[0057] Step 2, petroleum ether degreasing: disperse the methanol extract obtained in step 1 with 1 L of water, and extract with petroleum ether according to the ratio of 1:1 to obtain an aqueous raffinate solution for removing fat-soluble impurities;

[0058] Step 3, n-butanol extraction: the raffinate aqueous solution obtained in step 2 is extracted with saturated n-butanol according to the ratio of 1:1, and the extracted organic solution is concentrated under reduced pressure to obtain n-butanol extract;

[0059] Step 4, separation and purification: the n-butanol extract obtained in step...

Embodiment 3

[0061] The preparation method of the anti-inflammatory effective part of Panax notoginseng, the specific steps are as follows:

[0062] Step 1. Crushing and extracting: After crushing Pingbian notoginseng, leaching with methanol for 3 times according to the liquid-to-solid ratio of 1:8kg / L, the combined extracts were concentrated under reduced pressure to obtain 245.4g of methanol extract;

[0063] Step 2, petroleum ether degreasing: disperse the methanol extract obtained in step 1 with 1 L of water, and use petroleum ether to extract according to the ratio of 1:1.2 to obtain an aqueous raffinate solution for removing fat-soluble impurities;

[0064] Step 3, n-butanol extraction: the raffinate aqueous solution obtained in step 2 is extracted with saturated n-butanol according to the ratio of 1:1.2, and the extracted organic solution is concentrated under reduced pressure to obtain n-butanol extract;

[0065] Step 4, separation and purification: the n-butanol extract obtained i...

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Abstract

The invention relates to a preparation method and application of anti-inflammatory effective parts of Panax stipuleanatus, and belongs to the technical field of biological medicine. The preparation method comprises the following steps: after smashing Panax stipuleanatus, carrying out digestion on methyl alcohol, and carrying out vacuum concentration on a leach liquor to obtain a methyl alcohol extract; dispersing the obtained methyl alcohol extract with water, and extracting with petroleum ether to obtain an extraction raffinate water solution for removing fat-soluble impurities; extracting the obtained extraction raffinate water solution with saturated n-butyl alcohol, and extracting an organic solution to obtain an n-butyl alcohol extract through vacuum concentration; and separating theobtained n-butyl alcohol extract through silica gel column chromatography, carrying out elution by using a dichloromethane/methyl alcohol solvent system, and concentrating and drying an eluant to obtain the anti-inflammatory effective parts of Panax stipuleanatus. The effective parts of Panax stipuleanatus can be effectively prepared on large scale, and the application of Panax stipuleanatus in the preparation of anti-inflammatory drugs is realized.

Description

technical field [0001] The invention relates to a preparation method and application of an anti-inflammatory effective part of Panax notoginseng, belonging to the technical field of biomedicine. Background technique [0002] Pingbian notoginseng is a plant of the Araliaceae genus Pingbian notoginseng ( Panax stipuleanatus The roots and rhizomes of H.T. Tsai et K.M. Feng) are mainly produced in Pingbian, Maguan, Malipo and other areas in southeastern Yunnan. The folks are called wild notoginseng, bamboo knot seven, white notoginseng, fragrant thorns, and soil notoginseng. Traditionally, the roots and rhizomes are used as medicine. Folks believe that it has the effects of clearing blood stasis, relieving pain, promoting wound healing, stopping bleeding, and nourishing. At present, scholars at home and abroad have little research on Panax notoginseng, only on the preliminary chemical composition (Yang Chongren, et al. Yunnan Botanical Research, 1985, (01): 103-108.), plantin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P29/00
CPCA61K36/258A61K2236/15A61K2236/33A61K2236/51A61K2236/55
Inventor 曲媛舒盼盼杨野何勤敏崔秀明
Owner KUNMING UNIV OF SCI & TECH
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