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Preparation method of total saponins of panax stipuleanatus and application of anticoagulant drug

The technology of total saponins of Panax notoginseng and Panax notoginseng, applied in the field of biomedicine, can solve the problems of unreported antithrombotic disease of Pingbian Panax notoginseng, differences in functions and indications, etc., and achieve the effects of simple preparation method, small toxic and side effects, and high purity

Inactive Publication Date: 2019-10-01
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference in types of saponins in Panax notoginseng and Pingbian notoginseng will lead to differences in their functions and indications, and the two cannot be mixed
At present, scholars at home and abroad are focusing on their chemical composition, planting method, rapid identification method, conservation method, drying method, anti-inflammatory activity, anticancer activity, and blood lipid and blood sugar lowering activity. There is no report on the antithrombotic effect of Pingbian Sanqi

Method used

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  • Preparation method of total saponins of panax stipuleanatus and application of anticoagulant drug
  • Preparation method of total saponins of panax stipuleanatus and application of anticoagulant drug
  • Preparation method of total saponins of panax stipuleanatus and application of anticoagulant drug

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Effect test

Embodiment 1

[0027] Embodiment 1: a kind of preparation method of Panax notoginseng saponins, concrete steps are as follows:

[0028] Add 200g of Pingbian notoginseng powder (Pingbian notoginseng main root and rhizome powder) into 1L of extraction solvent (ethanol-water solution), extract by microwave for 20min at a temperature of 60°C, obtain residue and extract after filtration, and then The residue was extracted 3 times under the same extraction conditions, the combined extracts were then concentrated and dried under reduced pressure to obtain the extract, that is, the Radix Notoginseng extract (79.4g), and the extract was extracted with saturated n-butanol to obtain the n-butanol extract ( 64.8g), n-butanol extract adopts MCI column chromatographic separation (solvent system is ethanol by volume ratio: water=30:70,40:60,50:50,60:40,70:30,80:20,90 :10, 100:0 elution), carry out step elution with ethanol / water solvent system, collect the ethanol eluate whose ethanol volume fraction is 60...

Embodiment 2

[0033] Embodiment 2: a kind of preparation method of Panax notoginseng saponins, concrete steps are as follows:

[0034] Add 200g of Pingbian notoginseng powder (Pingbian notoginseng main root and rhizome powder) into 4L of extraction solvent (ethanol-water solution), extract by microwave for 25min at a temperature of 80°C, obtain residue and extract after filtration, and then in The residue was extracted 3 times under the same extraction conditions, the combined extracts were then concentrated and dried under reduced pressure to obtain the extract, that is, the Radix Notoginseng extract (98.4g), and the extract was extracted with saturated n-butanol to obtain the n-butanol extract ( 82.9g), n-butanol extract adopts MCI column chromatographic separation (solvent system is ethanol by volume ratio: water=30:70,40:60,50:50,60:40,70:30,80:20,90 :10, 100:0 elution), carry out step elution with ethanol / water solvent system, collect the ethanol eluate whose ethanol volume fraction is...

Embodiment 3

[0038] Embodiment 3: a kind of preparation method of Panax notoginseng saponins, concrete steps are as follows:

[0039] Add 200g of Pingbian notoginseng powder (Pingbian notoginseng main root and rhizome powder) into 6L of extraction solvent (ethanol-water solution), extract by microwave for 30min at a temperature of 100°C, obtain residue and extract after filtration, and then The residue was extracted 3 times under the same extraction conditions, the combined extracts were then concentrated and dried under reduced pressure to obtain the extract, that is, the Radix Notoginseng extract (83.5g), and the extract was extracted with saturated n-butanol to obtain the n-butanol extract ( 66.4g), n-butanol extract adopts MCI column chromatographic separation (solvent system is ethanol by volume ratio: water=30:70,40:60,50:50,60:40,70:30,80:20,90 :10, 100:0 elution), eluting with ethanol / water solvent system, collecting the ethanol eluate whose volume fraction of ethanol in the ethano...

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Abstract

The invention relates to a preparation method of total saponins of panax stipuleanatus and application of an anticoagulant drug, and belongs to the technical field of biological medicine. The preparation method comprises the steps that panax stipuleanatus powder is added into an extraction solvent, microwave extraction is carried out for 20-40 minutes at 60-100 DEG C, filtration is carried out toobtain residues and an extracting solution, then the residues are extracted 2-3 times in the same extraction condition, the extracting solutions are mixed, then decompression concentration is carriedout to obtain an extract, saturated n-butanol is used for extracting the extract to obtain a n-butanol extract, the n-butanol extract is separated by adopting MCI column chromatography, an ethanol / water solvent system is used for elution to obtain ethanol eluent, and the ethanol eluent is concentrated and dried to obtain the total saponins of the panax stipuleanatus. The total saponins of the panax stipuleanatus can be used for preparing a drug composition for treating thrombotic diseases and / or resisting blood coagulation.

Description

technical field [0001] The invention relates to a preparation method of Panax notoginseng total saponins and the application of anticoagulant drugs, belonging to the technical field of biomedicine. Background technique [0002] Thrombus is the deposition of fibrin and formed components of blood in blood vessels. Dysfunction of anticoagulant factors and fibrinolytic system can lead to diseases such as stroke, thrombosis and myocardial infarction. At present, the fatality rate of thrombosis is second only to nausea, tumor and myocardial infarction. Anticoagulants can inhibit the generation of thrombin and fibrin formation, thereby accelerating the dissolution of thrombus. The clinical drugs used for anticoagulation on the market have relatively large side effects and can easily induce diseases such as hemorrhage and hyperkalemia. However, natural anticoagulant drugs are rich in sources, have high efficiency and low toxicity, and have great potential in the treatment of throm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P7/02
CPCA61K36/258A61P7/02A61K2236/333A61K2236/39A61K2236/55A61K2236/51
Inventor 曲媛李璐茜杨野崔秀明杨晓艳
Owner KUNMING UNIV OF SCI & TECH
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