Molecularly imprinted photonic crystal film for rapidly detecting lysozyme, and preparation method and application thereof

A photonic crystal and molecular imprinting technology, applied in the field of material chemistry and analysis and detection, can solve the problems of low selectivity, complicated sample pretreatment, time-consuming, etc., achieve low cost, reduce detection cost and operation requirements, and have good reusability Effect

Active Publication Date: 2019-06-18
SHANGHAI E K M BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the detection method of lysozyme has achieved many results, but there are still problems such as complicated sample pretreatment, low selectivity, time-consuming, and cost.

Method used

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  • Molecularly imprinted photonic crystal film for rapidly detecting lysozyme, and preparation method and application thereof
  • Molecularly imprinted photonic crystal film for rapidly detecting lysozyme, and preparation method and application thereof
  • Molecularly imprinted photonic crystal film for rapidly detecting lysozyme, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0046] The specific preparation steps of the molecularly imprinted photonic crystal film for rapid detection of lysozyme of the present invention are as follows:

[0047] 1) After ultrasonically cleaning a 1×10×75 mm silicon wafer with deionized water, soak it in piranha solution (mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide, volume ratio 7:3) for 12 hours to remove surface impurities, After that, it was cleaned with deionized water and dried with nitrogen gas for later use;

[0048] 2) Monodisperse silica (SiO2) with a particle size of 240nm 2 ) Microspheres are ultrasonically dispersed into absolute ethanol to form a colloidal dispersion system, in which SiO 2 With a mass fraction of 2.0%, the hydrophilized matrix prepared in step 1) was inserted into the silica microsphere solution at an angle of 45°, and vertically self-assembled, and left to stand at 35°C under constant temperature and airtight conditions until the ethanol was completely vol...

Embodiment 2

[0055] The specific preparation steps of the molecularly imprinted photonic crystal film for rapid detection of lysozyme of the present invention are as follows:

[0056] 1) After ultrasonically cleaning a 1×10×75 mm silicon wafer with deionized water, soak it in piranha solution (mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide, volume ratio 7:3) for 12 hours to remove surface impurities, Afterwards, it was cleaned with deionized water, and dried with nitrogen gas for subsequent use to obtain a hydrophilic matrix;

[0057] 2) Monodisperse silica (SiO2) with a particle size of 220nm 2 ) Microspheres are ultrasonically dispersed into absolute ethanol to form a colloidal dispersion system, in which SiO 2 The mass fraction is 3.0%, the hydrophilized matrix prepared in step 1) is inserted into the silica microsphere solution at an angle of 90°, and vertical self-assembly is adopted, and it is left to stand at 35°C under a constant temperature and airtigh...

Embodiment 3

[0064] The specific preparation steps of the molecularly imprinted photonic crystal film for rapid detection of lysozyme of the present invention are as follows:

[0065] 1) After ultrasonically cleaning a 1×10×75 mm silicon wafer with deionized water, soak it in piranha solution (mixed solution of 98% concentrated sulfuric acid and 30% hydrogen peroxide, volume ratio 7:3) for 12 hours to remove surface impurities, After that, it was cleaned with deionized water and dried with nitrogen gas for later use;

[0066] 2) Monodisperse silica (SiO2) with a particle size of 220nm 2 ) Microspheres are ultrasonically dispersed into absolute ethanol to form a colloidal dispersion system, in which SiO 2 With a mass fraction of 2.0%, the hydrophilized matrix prepared in step 1) was inserted into the silica microsphere solution at an angle of 45°, and vertically self-assembled, and left to stand at 35°C under constant temperature and airtight conditions until the ethanol was completely vol...

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Abstract

The invention relates to a molecularly imprinted photonic crystal film for rapidly detecting lysozyme and belongs to the field of material chemistry and analysis and detection. The molecularly imprinted photonic crystal film is characterized in that the molecularly imprinted photonic crystal film which can realize specific identification and binding to lysozyme protein molecules and has the inverse opal structure is prepared by combining silica photonic crystal microspheres with the molecular imprinting technology. As the number of the bound lysozyme protein molecules increases, the macroporous structure on the film changes, the Bragg diffraction wavelength of the prepared molecularly imprinted photonic crystal film is made to realize red shift. According to the spectral response of the prepared molecularly imprinted photonic crystal gel membrane to the lysozyme, rapid detection of the concentration of the lysozyme in the sample can be realized. The molecularly imprinted photonic crystal film is advantaged in that properties of high sensitivity and selectivity and low detection limit are realized, and the molecularly imprinted photonic crystal film can be used as a biosensor used for rapidly detecting the lysozyme protein molecules in a biological sample.

Description

technical field [0001] The invention belongs to the technical field of material chemistry and analysis and detection, and relates to a molecularly imprinted photonic crystal film for rapid detection of lysozyme, a preparation method and application thereof, which can be used for biological sensing and optical devices. Background technique [0002] Lysozyme (Lysozyme) is a single-chain small molecule protein with a molecular weight of 14.3KDa and only 129 amino acids. Lysozyme has abnormal serum and body fluid lysozyme levels in humans, which is related to leukemia, kidney disease, conjunctivitis, meningitis and other diseases. The content of lysozyme in the human body has important physiological significance and has become an important clinical indicator in clinical diagnosis. At present, many achievements have been made in the research of lysozyme detection method, but there are still problems such as complicated sample pretreatment, low selectivity, time-consuming, and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/47
Inventor 张鑫李彦松张浩
Owner SHANGHAI E K M BIOTECH
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