Near-infrared Ag2S nanometer assembly probe, and preparation method and applications thereof

Abstract

The invention provides a near-infrared Ag2S nanometer assembly probe. The near-infrared Ag2S nanometer assembly probe comprises a plurality of monomers M1 and M2 which are assembled based on HCR reaction; the monomer M1 is prepared through assembling of DNA-Ag2S nanodot and nucleic acid sequence such as H1 represented by SEQ ID NO.2, wherein the DNA sequence is represented by SEQ ID NO.1; the monomer M2 is prepared through assembling of an aptamer such as the aptamer represented by SEQ ID NO.3 and nucleic acid sequence such as H2 represented by SEQ ID NO.4. The invention also provides a preparation method of the near-infrared Ag2S nanometer assembly probe. The raw materials are easily available; the conditions are mild; normal temperature reaction is adopted; no heating is needed; productquality is stable. The invention also provides application method and applications of the near-infrared Ag2S nanometer assembly probe in detection of circulating tumor cells. The near-infrared Ag2S nanometer assembly probe is used in detection of circulating tumor cells, is high in specific identification capacity and sensitivity, can be used for further analysis of target cells in whole blood, and is promising in application prospect in cancer clinical diagnosis, postoperative prevention, and treatment.

Description

technical field

[0001] The invention belongs to the field of biological analysis, and in particular relates to a near-infrared Ag 2 S nanometer assembly probe, its preparation method and its application in detection of circulating tumor cells. Background technique

[0002] Circulating tumor cells (CTCs) are a general term for tumor cells present in peripheral blood, and their expression levels in blood are closely related to the prognosis and survival of cancer patients. The detection of CTCs is of great significance for the early diagnosis of metastases, cancer prognosis, treatment monitoring and metastasis diagnosis. But the detection and analysis of CTCs is not easy because there is no clear distinction between CTCs and other blood cells. In addition, the number of CTCs in peripheral blood is very small, usually in the range of 10 9 Only one in every white blood cell. Therefore, highly efficient isolation and detection of CTCs from whole blood is a challenging subject...

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