Electrochemical AC impedance biosensor for detecting MMP-14 and preparation method thereof

An MMP-14, biosensor technology, applied in the field of electrochemical AC impedance biosensor and its preparation, can solve the problems of lack of specificity of biosensing technology, difficult application to detect MMPs, etc., and achieve high selectivity and high sensitivity detection, The effect of accurate detection results

Inactive Publication Date: 2019-06-21
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, peptide cleavage fragments often bind to fluorescent or electroactive molecules non-specifically, resulting in false positive signals
Therefore, the aforementioned biosensing techniques are difficult to apply clinically to detect active-site-directed MMPs due to lack of specificity.

Method used

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  • Electrochemical AC impedance biosensor for detecting MMP-14 and preparation method thereof
  • Electrochemical AC impedance biosensor for detecting MMP-14 and preparation method thereof
  • Electrochemical AC impedance biosensor for detecting MMP-14 and preparation method thereof

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preparation example Construction

[0029] A method for preparing an electrochemical AC impedance biosensor for detecting MMP-14, the specific preparation steps are as follows:

[0030] (1) Pretreatment of gold electrodes

[0031] The gold electrode was polished to a mirror surface with 1.0 μM, 0.3 μM and 0.05 μM aluminum oxide on a soft polishing cloth, and the surface of the electrode was rinsed with deionized water after polishing. The gold electrodes were then ultrasonically cleaned in ethanol and deionized water for 2-5 min, respectively. The cleaned gold electrode was used as the working electrode, the platinum wire electrode was used as the counter electrode, and Ag / AgCl was used as the reference electrode (saturated KCl) to form a three-electrode system at 0.1-0.5M H 2 SO 4 The cyclic voltammetry scan was carried out in the solution until the cyclic voltammetry spectrum was stable. The scanning potential range is -0.3V~+1.5V, and the scanning rate is 0.1V.S -1 .

[0032] (2) Configuration of probes ...

specific Embodiment 1

[0046] A preparation method of an electrochemical AC impedance biosensor for detecting MMPs, the MMPs detected in the present invention is MMP-14, and the specific preparation steps are as follows:

[0047] (1) Pretreatment of gold electrodes

[0048] a. The gold electrode was polished to a mirror surface with 1.0 μM, 0.3 μM and 0.05 μM aluminum oxide on a soft polishing cloth, and the surface of the electrode was rinsed with deionized water after polishing. The gold electrodes were then ultrasonically cleaned in ethanol and deionized water for 5 min, respectively. The cleaned gold electrode was used as the working electrode, the platinum wire electrode was used as the counter electrode, and Ag / AgCl was used as the reference electrode (saturated KCl) to form a three-electrode system at 0.5 M H 2 SO 4 The cyclic voltammetry scan was carried out in the solution until the cyclic voltammetry spectrum was stable. The scanning potential range is - 0.3V~+1.5V, and the scanning rat...

specific Embodiment 2

[0055] A preparation method of an electrochemical AC impedance biosensor for detecting MMPs, the MMPs detected in the present invention is MMP-14, and the specific preparation steps are as follows:

[0056] (1) Pretreatment of gold electrodes

[0057] a. The gold electrode was polished to a mirror surface with 1.0 μM, 0.3 μM and 0.05 μM aluminum oxide on a soft polishing cloth, and the surface of the electrode was rinsed with deionized water after polishing. The gold electrodes were then ultrasonically cleaned in ethanol and deionized water for 2 min, respectively. The cleaned gold electrode was used as the working electrode, the platinum wire electrode was used as the counter electrode, and Ag / AgCl was used as the reference electrode (saturated KCl) to form a three-electrode system at 0.1 M H 2 SO 4 The cyclic voltammetry scan was carried out in the solution until the cyclic voltammetry spectrum was stable. The scanning potential range is - 0.3V ~ +1.5V, and the scanning r...

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Abstract

The invention provides an electrochemical AC impedance biosensor for detecting MMP-14 and a preparation method thereof. The polypeptide inhibitor is dissolved in the Tris-HCl buffer solution so as toobtain the MMP-14 specific identification probe solution, wherein that amino acid sequence of the polypeptide inhibitor is: GYPKSALR-(CH2) 6-Cys; the gold electrode is immersed into the MMP-14 specific identification probe solution to perform self-assembling modification and then rinsed by the Tris-HCl buffer solution so as to obtain the IVSC / GE electrode; and the MCH solution is dropped into theIVSC / GE electrode surface for sealing and the electrode surface is rinsed so as to obtain the electrochemical AC impedance biosensor for detecting MMP-14. The electrochemical AC impedance biosensor has high specificity.

Description

technical field [0001] The invention belongs to the field of medical devices, and relates to an electrochemical AC impedance biosensor for detecting MMP-14 and a preparation method thereof. Background technique [0002] Matrix metalloproteinases (MMPs) are enzymes responsible for degrading most of the extracellular matrix during tissue remodeling and angiogenesis under physiological and pathological conditions. It has been reported that MMPs are mainly overexpressed in diseases such as vascular diseases, cancer, skeletal diseases and neurodegenerative diseases. MMPs can be used as markers of colorectal cancer, thyroid cancer, bladder cancer, breast cancer, neurodegenerative diseases, immune diseases and cardiovascular diseases. Therefore, the development of sensitive and precise MMPs sensing technology is closely related to the treatment of related diseases, for example, cancer diagnosis and drug screening. [0003] In recent years, activity analysis of MMP-9, MMP-3, MMP-7...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
Inventor 马芬陈玉孙利娜
Owner NORTHWEST UNIV
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