Production method of clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe

A technology of luminescent materials and molecular imprinting, which can be used in cross-cutting fields and can solve the problems of life-threatening, long residual time, toxic and side effects, etc.

Active Publication Date: 2015-08-05
BOHAI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, CLB is easy to accumulate in animals, and the residual amount in the lung, liver, kidney and other organs, as well as muscle and adipose tissue is relatively large, and the residual time is long, and it can accumulate in the human body through the food chain, causing toxic and side effects on the body. Manifested as arrhythmia, muscle tremor, dizziness, fatigue, etc., which can be life-threatening in severe cases

Method used

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  • Production method of clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe
  • Production method of clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe
  • Production method of clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe

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[0032] The invention relates to a clenbuterol molecular imprinting-up-conversion luminescent material-labeled fluorescent probe preparation method, which is characterized in that: the up-conversion luminescent material YF 3 :Yb 3+ ,Er 3+ As a carrier, a fluorescent probe formed by covalently preparing clenbuterol molecularly imprinted polymers, this fluorescent probe combines the high selectivity of molecularly imprinted polymers and the special fluorescence emission characteristics of up-conversion luminescent materials. The preparation steps are as follows:

[0033] (1) Set Y(NO 3 ) 3 , Yb(NO 3 ) 3 , Er(NO 3 ) 3 Mix with distilled water, stir until completely dissolved, add EDTA and NaF solution, continue stirring for 5-10 minutes, transfer the above mixture solution to a hydrothermal reaction kettle, and use NaOH or HNO 3 Solution Adjust the pH value of the solution to 3, then tighten the hydrothermal reactor and place it under the condition of 180°C for hydrotherma...

Embodiment 1

[0048] A clenbuterol molecular imprinting-up-conversion luminescent material fluorescent probe preparation method is the up-conversion luminescent material YF 3 :Yb 3+ ,Er 3+ As a carrier, combined with the fluorescent probe formed by clenbuterol molecular imprinting prepared by the covalent bond method, the steps are as follows:

[0049] (1) Preparation of up-conversion luminescent materials:

[0050] 6.6 mmol Y(NO 3)3 (purity 99.99%), 1.69 mmol Yb(NO 3 ) 3 (purity 99.99%), 0.169 mmol Er(NO 3 ) 3 (purity 99.99%) mixed with 13.0~18.0 mL distilled water, stirred until completely dissolved, then added 33.0 mL EDTA (6.6 mmol) solution and 33.0 mL NaF (46 mmol) solution, continued stirring for 5~10 minutes, then transferred the above mixture solution To the hydrothermal reactor, use NaOH or HNO 3 Solution Adjust the pH value of the solution to 3, then tighten the hydrothermal reactor and place it under the condition of 180°C for hydrothermal reaction for 24 hours to obtain...

Embodiment 2

[0058] A clenbuterol molecular imprinting-upconversion luminescent material fluorescent probe preparation method, in step (2), mix 200 mL of 3 mol / L sodium hydroxide solution with 5 g of fluorescent probe, heat and reflux for 48 hours; prepare Clenbuterol For terol derivatives, dissolve 10 mmol of clenbuterol in 55 mL of dichloromethane, add 35 mmol of triethylamine to the solution, drop 25 mmol of methacryloyl chloride, and react overnight at room temperature; other conditions are the same as in the examples 1.

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Abstract

The invention relates to a production method of a clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe. The probe is a novel fluorescence probe produced through combining an upconversion luminescent material YF3:Yb<3+>, Er<3+> as a carrier with a covalent bond technology prepared clenbuterol molecularly imprinted polymer. The probe combines the high specific identification performance of the molecularly imprinted polymer with the high sensitivity of the upconversion luminescent material. The production method comprises the following steps: preparing the upconversion luminescent material, preparing the clenbuterol molecularly imprinted-upconversion luminescent material fluorescence probe, and eluting clenbuterol molecules. The fluorescence probe produced in the invention emits visible light under the excitation of infrared light, and has the advantages of high sensitivity, strong specificity and small interference. The production method has the advantages of simplicity, good reappearance, and good application prospect in selective identification and detection of clenbuterol in a practical sample.

Description

technical field [0001] The invention belongs to the interdisciplinary fields of biology, materials, and biological detection, and mainly relates to the preparation technology of fluorescent probes of up-conversion luminescent materials, in particular to a preparation method of clenbuterol molecular imprinting-up-conversion luminescent material-labeled fluorescent probes. Background technique [0002] Upconversion luminescence, namely Anti-Stokes luminescence (Anti-Stokes), is the process of converting long-wavelength light into short-wavelength light emission. Its mechanism includes excited state absorption, energy transfer upconversion and photon avalanche. Up-conversion luminescent materials refer to materials with up-conversion luminescence properties, which can emit ultraviolet or visible light under the excitation of near-infrared light or infrared light, and are characterized by the absorbed photon energy being lower than the emitted photon energy, also known as Anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85G01N21/64
Inventor 汤轶伟高子媛高雪高静纹兰建兴魏立巧李译励建荣
Owner BOHAI UNIV
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