Construction method for breeding pedigree of lutraria sieboldii

The invention relates to a technology for the construction method and the application of the strych rex clams in the field of construction of the breeding family of the strych rex clams. Sexual and Operational Effects

Active Publication Date: 2018-08-28
广西海洋研究所有限责任公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Since the breeding environment of Otter clams is subtidal open waters, the resources are complex and marking is difficult, and no effective family selection mechanism has been established in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example

[0021] (1) Artificial directional insemination

[0022] In November 2015, 100 high-quality mature otter clam broods were selected, dissected to remove the half-shell, lightly squeezed the gonad of the clam body, sucked a small amount of squeezed liquid at the twisted part of the shell, and used conventional methods to distinguish sex, preferably healthy 1. 5 male individuals with full gonads and 25 female individuals. The semen-containing fluid and egg-containing fluid extracted from the individuals were respectively inhaled into sterilized seawater with a volume of 200ml and a specific gravity > 1.016 in a container, respectively. Numbering, the seawater containing semen and egg fluid is mixed separately at a ratio of 1:15~1:20, and at the same time, 28% ammonia solution is added to the volume fraction of ammonia water to 2×10 -5 , mix well, the number of sperm around the eggs is in the range of 5-20 under the microscope, and the color of more than 80% of the egg cells is uni...

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PUM

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Abstract

The invention discloses a construction method for a breeding pedigree of lutraria sieboldii. The lutraria sieboldii is a rare bivalve shellfish, and excessive breeding causes problems such as germplasm degeneration and frequent diseases; and pedigree breeding becomes a first choice for industrial development, but because of a subtidal open water area breeding environment of the lutraria sieboldii,complex resources, and a large number of individuals to be marked, common marking and molecular marking are difficult, and an effective pedigree breeding mechanism is not established in China. The method disclosed by the invention adopts the technical steps of artificial directional insemination, breeding, pedigree individual marking and cultivation to solve the difficult problem of pedigree construction of pedigree breeding of the lutraria sieboldii, can quickly realize the pedigree breeding by combining genetic polymorphism analysis, and provides technical references for pedigree breeding of the lutraria sieboldii and related subtidal bivalve shellfish.

Description

technical field [0001] The invention relates to a method for constructing a breeding family of otter clams schneri, and belongs to the technical field of bivalve mollusc breeding in aquatic science. Background technique [0002] Shi's otter clam ( Lutraria sieboldii ) is a rare bivalve mollusk in the southern coast of my country. It mainly lives in the subtidal shallow sea area with a water depth of 2m~50m. Due to the development of diving fishing, the aquaculture industry of Shishi's otter clam has developed rapidly, and at the same time, germplasm decline and disease have occurred Frequent occurrence and other problems, so family breeding has become the first choice for industrial development. Since the breeding environment of Otter clam shishii is open water in the subtidal zone, the resources are complex and marking is difficult, and an effective family selection mechanism has not been established in China. For this reason, according to the biological characteristics of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 邹杰彭慧婧杨家林聂振平
Owner 广西海洋研究所有限责任公司
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