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Molecular mark for affecting early-stage growth of sheep as well as detection method and application of molecular mark

A molecular marker, sheep technology, applied in the field of molecular biology, can solve the problems of slow genetic progress, low accuracy of growth trait phenotype selection, affecting the early body weight of sheep, etc.

Active Publication Date: 2020-03-27
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the defects of low accuracy of growth trait phenotype selection and slow genetic progress in sheep breeding, and provide a molecular marker that affects the early body weight of sheep

Method used

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  • Molecular mark for affecting early-stage growth of sheep as well as detection method and application of molecular mark
  • Molecular mark for affecting early-stage growth of sheep as well as detection method and application of molecular mark
  • Molecular mark for affecting early-stage growth of sheep as well as detection method and application of molecular mark

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, PLAG1 gene NC_040260.1:g.39071254C / T molecular marker selection method for early growth of Hu sheep Materials and methods:

[0031] (1) Flock

[0032] The 352 Hu sheep samples were fed and managed by Jiangsu Xilai Yuanecological Agriculture Co., Ltd., and the nutrition and management levels were consistent. The body weight data were provided by Jiangsu Xilai Yuanecological Agriculture Co., Ltd.

[0033] (2) Method

[0034] 1. Sample collection

[0035] Ear tissue samples were collected from each individual, brought back to the laboratory in an ice bucket, and stored at -20°C.

[0036] 2. Extraction of DNA

[0037] DNA was extracted by conventional phenol and chloroform methods, and OD was determined by electrophoresis 260 / 280 Check DNA quality.

[0038] 3. Amplification of the sequence containing PLAG1 gene NC_040260.1:g.39071254

[0039] According to the sequence NC_040260.1, use Primer Primer 5 to design a pair of specific primers (sequence P1: CC...

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Abstract

The invention discloses a molecular mark for affecting early-stage growth of sheep as well as a detection method and application of the molecular mark. The molecular marker for affecting the early-stage body weight of the sheep is located in a sheep PLAG1 gene NC 040260.1:g.39071254 locus, and has C / T polymorphism, and the birth weight and weaning weight of the CT type sheep are significantly higher than those of CC type sheep. The method for screening early-stage fast-growth sheep breeds (lines) includes the steps of detecting the C / T polymorphism of the sheep PLAG1 gene NC 040260.1:g.39071254 locus, and selecting CT type individuals for seed retention. The polymorphic locus can be used for early-stage auxiliary selection of sheep growth traits, improve the selection accuracy, and speed up the breeding process.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a molecular marker of pleomorphic adenoma gene 1 (PLAG1), which affects the early growth of sheep, a detection method and an application thereof. Background technique [0002] PLAG1 is a member of the pleomorphic adenoma gene family and belongs to the zinc finger transcription factor. It was first discovered that it was highly expressed in pleomorphic adenoma and promoted the occurrence of pleomorphic adenoma. Later, it was found that it can promote the occurrence of various tumors such as lipoblastoma and hepatoblastoma. Highly expressed PLAG1 binds to the IGF2 P3 promoter, upregulates the expression of IGF2, promotes cell proliferation and anti-apoptosis (Voz ML et al, 2000), and IGF2 is necessary for normal fetal growth (Kadakia R et al, 2016) . [0003] Hensen K et al. (2004) showed that PLAG1 knockout mice had fetal growth retardation and affected postnatal growth. The 11.5...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曹少先王悦李隐侠钱勇王慧利孟春花张俊桂红兵仲跻峰
Owner JIANGSU ACAD OF AGRI SCI
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