SSR primer group developed based on multiple transcriptome sequences of color group zantedeschia aethiopica and acquisition method and application

Abstract

The invention discloses an SSR primer group developed based on multiple transcriptome sequences of color group zantedeschia aethiopica and acquisition method and application. The method comprises the following steps of firstly, screening polymorphic SSR primers from transcriptome splicing sequences of three varieties of the color group zantedeschia aethiopica by utilizing a CandiSSR method, determining hundreds of polymorphic SSR primers after experimental verification, and then carrying out polymorphic analysis on more than 160 parts of color group zantedeschia aethiopica varieties by utilizing 35 pairs of screened SSR primers with high polymorphism. In addition, on the basis of the research, 160 core germplasm of the color group zantedeschia aethiopica is constructed through a maximization strategy, finally, 30 optimal representative parents of the color group zantedeschia aethiopica are screened out, and good help is provided for screening of core parents of the color group zantedeschia aethiopica, improvement of breeding efficiency and accelerated cultivation of new varieties with proprietary intellectual property rights in the future.

Description

technical field [0001] The invention belongs to the molecular marker technology, and in particular relates to an SSR primer set developed based on multiple transcriptome sequences of the color group calla lily, an acquisition method and an application. Background technique [0002] In recent years, the development and application of second-generation sequencing technology has gradually broken the bottleneck of the difficulty and high cost of obtaining transcribed EST sequences. Sequencing technology platforms represented by Illumina / Solexa Genome Analyzer, Roche / 454FLX, and AppliedBiosystems SOLID system can capture DNA sequences by capturing the markers of newly synthesized ends. The sequencing-by-synthesis method can be used in a very short time and at the lowest cost. Get the maximum amount of data. Although compared with the traditional Sanger sequencing method, the second-generation sequencing sequence is relatively short, which will increase the difficulty and calcula...

AI Technical Summary

Problems solved by technology

First, the workload will be heavy due to the large number of varieties. Second, it will increase the mutation risk of excellent varieties. Third, the breeding time will be extended, because it will take at least 3 years from the emergence of tissue cultured seedlings to conventional cross breeding through flowering and pollination. Fourth, It is the cultivation of bulbs that will also degenerate

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