Molecular marker linked with major quantitative trait locus (QTL) of hundred-kernel weight of arachis hypogaea and application of molecular marker

A technique based on molecular markers and all kinds of kernels, which is applied in the fields of molecular genetics and crop molecular breeding, can solve the problems of general effect, low phenotypic contribution rate of molecular markers, and large QTL intervals

Active Publication Date: 2021-04-09
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, scholars at home and abroad have some reports on the QTL mapping of peanut weight (Wang et al., 2019; Huang et al., 2015; Fonceka et al., 2012; Li et al., 2011; Ravi et al., 2011 ), but the QTL identifi

Method used

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  • Molecular marker linked with major quantitative trait locus (QTL) of hundred-kernel weight of arachis hypogaea and application of molecular marker
  • Molecular marker linked with major quantitative trait locus (QTL) of hundred-kernel weight of arachis hypogaea and application of molecular marker
  • Molecular marker linked with major quantitative trait locus (QTL) of hundred-kernel weight of arachis hypogaea and application of molecular marker

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Experimental program
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Effect test

Embodiment 1

[0043] This embodiment provides a method for screening molecular markers linked to the main effect QTL locus of peanut weight, which specifically includes the following steps:

[0044] 1. Construct a recombinant inbred line (RIL) population with Zhonghua 16 and sd-H1 as parents. The female parent, Zhonghua 16, is a high-yield, high-oil peanut variety with pearl bean-shaped large fruits, and the male parent, sd-H1, is a multi-grained small-fruited peanut variety. For low-yielding peanut varieties, the two parents have large differences in agronomic traits such as grain weight (100-kernel weight), such as figure 1 shown.

[0045]2. Zhonghua 16, sd-H1 and F6 (2016), F7 (2017), F8 (2018) generation RIL population 242 strains were sown in two experimental bases in Wuhan and Yangluo three times in completely randomized blocks After the material matures naturally, it is harvested and dried in the sun. According to the standard in the "Peanut Germplasm Resources Description Specifica...

Embodiment 2

[0050] In this embodiment, the linkage between the two molecular markers related to the weight of 100 kernels and the traits in peanut varieties obtained in Example 1 is verified, as follows:

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Abstract

The invention relates to the technical field of molecular genetics and crop molecular breeding, in particular to a molecular marker linked with a major quantitative trait locus (QTL) of the hundred-kernel weight of arachis hypogaea and application of molecular marker. The molecular marker includes a molecular marker Ah011476, and/or, a molecular marker Ah011478; the molecular marker Ah011476 is the 14206230 site on chromosome B06 of arachis hypogaea, and polymorphism is G or T; and the molecular marker Ah011478 is the 17650027 site on chromosome B06 on the arachis hypogaea, and polymorphism is C or A. According to the invention, the molecular markers linked with the arachis hypogaea hundred-kernel weight major QTL are obtained by screening, and the molecular markers can be used for effectively identifying the hundred-kernel weight of the arachis hypogaea, and are used for genetic improvement of the arachis hypogaea to obtain an arachis hypogaea variety with high hundred-kernel weight.

Description

technical field [0001] The invention relates to the technical fields of molecular genetics and crop molecular breeding, in particular to a molecular marker linked to a main effect QTL locus of peanut weight and its application. Background technique [0002] Peanut (Arachis hypogaea L.) is one of the most important oil crops and economic crops. It has a large planting area and high yield. It can be used for oil extraction and is one of the important sources of edible oil. Peanut is also an important raw material for food processing, and food consumption accounts for more than 35% of the total output. At present, increasing the yield per unit area is one of the effective ways to further increase the supply of peanut raw materials. Studies have shown that peanut kernel weight has the greatest impact on yield and is the most important peanut yield component factor. Therefore, it is of great importance to carry out the QTL mapping research on the kernel weight of peanuts, ident...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113
Inventor 王志慧廖伯寿雷永晏立英陈玉宁淮东欣王欣康彦平
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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