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Photochromic dynamic multi-substrate detection microchip and polymorphism analysis method

A photochromic and microchip technology, used in material excitation analysis, chemical instruments and methods, fluorescence/phosphorescence, etc., can solve the problems that limit the development and rapid preparation of high-efficiency detection chips, and achieve excellent environmental friendliness and chemical economy. The effect of sex, simple process

Inactive Publication Date: 2015-03-25
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method requires a large number of series of organic compounds as sensors, and the construction of sensor arrays for different detection substrate types; and the whole process involves experienced combinatorial chemical design, multi-step synthesis, compound effective responsiveness screening, sensor chip Complex and cumbersome procedures such as design and processing have largely restricted the development and rapid preparation of high-efficiency detection chips, making multi-substrate high-efficiency detection chips still in the laboratory and research stage

Method used

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  • Photochromic dynamic multi-substrate detection microchip and polymorphism analysis method
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  • Photochromic dynamic multi-substrate detection microchip and polymorphism analysis method

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Experimental program
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Effect test

Embodiment 1

[0031] 1) Select the metal cation as the substance to be detected. In the absence of light, the AlCl 3 , FeCl 3 , CoCl 2 , NiCl 2 , CuCl 2 , ZnCl 2 , HgCl2 , CdCl 2 , CaCl 2 , MgCl 2 Aqueous solutions of metal cations were spotted onto photochromic spirooxazine chips.

[0032] 2) Under the excitation of ultraviolet lamp (365nm), use a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under the filter of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm wavelength respectively.

[0033] 3) Ultraviolet irradiates the detection chip for ten minutes, and uses a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under filter filters with wavelengths of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm respectively.

[0034] 4) The detection chip was irradiated with visible light for ten minutes, and the fluorescence imaging and intensity recording were carried out on the chip under the filters of 450nm, 480nm...

Embodiment 2

[0037] 1) Amino acid is selected as the substance to be detected, and it is detected under the cooperation of spirooxazine and metal cations. In the absence of light, the FeCl 3 , CuCl 2 , HgCl 2 The metal cation aqueous solution is respectively dot-stained on the photochromic spirooxazine chip to make three detection chips. Ala, Arg, Cys, Gly, Lys, Pro, Met, Thr, Ser, His, Val, Tyr, Asn, Glu, Trp, Gln, Ile, Phe, Asp, Leu were stained on three chips.

[0038] 2) Under the excitation of ultraviolet lamp (365nm), use a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under the filter of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm wavelength respectively.

[0039] 3) Ultraviolet irradiates the detection chip for ten minutes, and uses a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under filter filters with wavelengths of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm respectively.

[0040] 4) The ...

Embodiment 3

[0043] 1) Dipeptide is selected as the substance to be detected, and detection is performed under the cooperation of spirooxazine and metal cations. In the absence of light, the FeCl 3 , CuCl 2 , HgCl 2 The metal cation aqueous solution was spot-stained on the photochromic spirooxazine chip respectively to make three detection chips. Spotting Val-Phe, Gly-Ala, His-Ala, Ala-His, Phe-Pro, Pro-Gly on three chips.

[0044] 2) Under the excitation of ultraviolet lamp (365nm), use a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under the filter of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm wavelength respectively.

[0045] 3) Ultraviolet irradiates the detection chip for ten minutes, and uses a fluorescence scanner to perform fluorescence imaging and intensity recording on the chip under filter filters with wavelengths of 450nm, 480nm, 505nm, 535nm, 570nm and 605nm respectively.

[0046] 4) The detection chip was irradiated with visibl...

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Abstract

The invention belongs to the field of photochromic material and multi-substrate analysis. Photochromic molecules are introduced into a sensor array so as to prepare an efficient universal multi-substrate detection analysis microchip. According to the invention, photochromic molecules, namely spiropyrane / spirooxazine, are utilized, the response optical difference of the microchip to various metal cations under different photostimulation conditions (light resistance, ultraviolet light illumination and visible light illumination) is analyzed comprehensively, and an array chip consisting of a single spiropyrane or spirooxazine compound is designed. Fluorescence and absorption chemical information is recorded via a plurality of channels, and difference analysis and identification can be carried out on different kinds of metal cations as multiple as possible by utilizing high-flux statistics methods such as principal component analysis (PCA), hierarchic classification analysis (HCA) and linear difference analysis (LDA) and the like. According to the invention, the single chemical sensor is used for detecting and analyzing multiple substrates by constructing the photochromic dynamic multi-substrate detection microchip, and the product has broad-spectrum universality and great operability for multi-substrate recognition and detection at various complex environments.

Description

technical field [0001] The invention belongs to the field of micro-nano materials and photochemical analysis, in particular to a high-efficiency general-purpose multi-substrate detection and analysis microchip prepared by utilizing photochromic intelligent responsive materials. Background technique [0002] The detection and analysis of multiple substrates and complex environments has very realistic research significance and application prospects for industry, food monitoring and living organism analysis. Although humans have made great progress in the detection sensitivity and specific recognition of many single substrates, the response performance of a single chemical sensor compound is usually relatively single, and it is impossible to realize the recognition and analysis of multiple detection substrates. Based on combinatorial chemical design and compound array chip technology, scientists such as Anslyn have developed a sensor array that uses a variety of sensor compound...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/63G01N21/64
Inventor 秦萌李风煜李一凡黄羽宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI
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