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Fingerprint establishment method and detection method of traditional Chinese medicine composition

A fingerprint and establishment method technology, which is applied in the fingerprint establishment method and detection field of traditional Chinese medicine composition, can solve the problems that the quality of medicine and treatment effect are difficult to be guaranteed, and achieve the effect of ensuring the safety and treatment effect of medicine and enriching chemical information

Pending Publication Date: 2021-12-07
广州康臣药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on this prescription, and the existing information cannot fully reflect the chemical characteristics and quality of this prescription, making it difficult to guarantee the quality and therapeutic effect of this prescription.

Method used

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  • Fingerprint establishment method and detection method of traditional Chinese medicine composition
  • Fingerprint establishment method and detection method of traditional Chinese medicine composition
  • Fingerprint establishment method and detection method of traditional Chinese medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] The fingerprint collection method of embodiment 1 Chinese medicine composition

[0138] 1. Instruments, reagents and reagents

[0139] (1) Instrument

[0140] AgiLent 1260 high performance liquid chromatography;

[0141] AdvanceBio Peptide Mapping (2.1×250mm, 2.7μm) chromatographic column;

[0142] Ultrasonic cleaning machine (Shenzhen Jiemeng Cleaning Equipment Co., Ltd., JP-060);

[0143] BS224S electronic balance (Sartorius);

[0144] Synergy ultrapure water machine (Guangzhou Dongrui Technology).

[0145] (2) Reagents

[0146] Acetonitrile (chromatographically pure, Merck company);

[0147] Ultrapure water (self-made in the laboratory);

[0148] Methanol (analytical grade, Guangzhou Chemical Reagent Factory).

[0149] (3) Test drug

[0150] Yishen Huashi Granules (Guangzhou Kangchen Pharmaceutical Research Co., Ltd., batch numbers: 20180303, 20180301, 20180302, 20180401, 20180402, 20180403, 20180404, 20180501, 20180502, 20180503). The preparation method of...

Embodiment 2

[0214] The detection method of embodiment 2 Chinese medicine composition

[0215] Take Yishen Huashi granules to be tested (Guangzhou Kangchen Pharmaceutical Research Co., Ltd., batch numbers: 20201207, 20201208, 20201209, 20201218, 20201219, 20201220), and prepare the to-be-tested product according to the method under "Determination of the preparation method of the test product" in Example 1. Test samples.

[0216] Detect by the method under the item "determination of chromatographic conditions" in Example 1, obtain the fingerprints of Yishen Huashi granules to be tested, and adopt the "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System" for the fingerprints of 6 batches of Yishen Huashi granules. 2004A Edition" to perform common peak matching, and calculate the similarity between the fingerprints of the 6 batches of Yishen Huashi Granules, the results are shown in Figure 10 , Table 9.

[0217] Table 9 Similarity of fingerprints of 6 batches of Yishe...

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Abstract

The invention relates to a fingerprint spectrum establishment method and a detection method of a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials of ginseng, astragalus membranaceus, rhizoma atractylodis macrocephalae, poria cocos, rhizoma alismatis, rhizoma pinelliae preparata, notopterygium root, radix angelicae pubescentis, radix saposhnikoviae, radix bupleuri, coptis chinensis, radix paeoniae alba, pericarpium citri reticulatae, honey-fried licorice root, fresh ginger and Chinese dates. The establishment method of the fingerprint spectrum comprises the following steps of preparing a reference medicinal material solution and a test solution; detecting the reference medicinal material solution and the test solution by high performance liquid chromatography; the mobile phase adopted by the high performance liquid chromatography being acetonitrile and water, and the elution mode being gradient elution. The method is advantaged in that the fingerprint spectrum of the traditional Chinese medicine composition constructed by the method can effectively characterize the traditional Chinese medicine composition, provides richer chemical information and more comprehensive basis for quality evaluation of the traditional Chinese medicine composition, and better guarantees medication safety and treatment effect of patients.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for establishing a fingerprint of a traditional Chinese medicine composition and a detection method. Background technique [0002] The traditional Chinese medicine composition contains a variety of sources and complex herbal medicines. The efficacy of the traditional Chinese medicine composition does not come from a single active ingredient, but the result of the joint action of the raw materials of the entire Chinese medicine composition. The content of the active ingredients in the traditional Chinese medicine composition , composition ratio and other differences will have an impact on the efficacy. Based on the complexity of the composition of traditional Chinese medicine, any single active ingredient or index ingredient is difficult to effectively evaluate the quality of traditional Chinese medicine. When evaluating the quality of traditional Chinese medicine comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/88
CPCG01N30/8686G01N30/88G01N2030/027G01N2030/8809
Inventor 王汝上段子浩钟铖李政海段婷婷钟泓玲麦瑞芬
Owner 广州康臣药业有限公司
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