Method for developing ramie genome SNP marks on large scale and primer developed by method
A genome and large-scale technology, applied in the field of genetic engineering, can solve problems such as ramie SNP markers that have not yet been developed, and achieve the effect of low cost, high efficiency and fast speed
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] (1) Determine the enzyme digestion plan, gel cutting range, sequencing volume, etc. to ensure the density and uniformity of molecular marker development to ensure that the expected experimental purpose is achieved.
[0020] The DNA of Ramie No.1 and Hejiang Qingma was extracted.
[0021] Enzyme digestion scheme selection
[0022] According to the selection principle of closely related species, the cannabis genome was finally selected as the reference genome for enzyme digestion prediction.
[0023] Ramie species information and related species information are as follows:
[0024] Ramie information: Genome size is 716Mb, GC content is 49%;
[0025] Related species cannabis information: Genome size is 757Mb, GC content is 24%, download address:
[0026] ftp: / / ftp.ncbi.nlm.nih.gov / genbank / genomes / Eukaryotes / plants / Cannabis_sativa / canSat3 / .
[0027] Candidate Digestion Protocol Information
[0028] The cannabis genome was systematically analyzed using enzyme digestio...
Embodiment 2
[0055] Primer polymorphism detection
[0056] 1 Materials and methods
[0057] 1.1 Materials
[0058] 4 ramie germplasm resources (Zhongzhu No. 1, Hejiang Qingma, Zhongzhu No. 2, Hongpi Honghua).
[0059] 1.2 Method
[0060] 1.2.1 Genomic DNA extraction
[0061] The new shoots of 4 ramie germplasms (Zhongzhu No. 1, Hejiang Qingma, Zhongzhu No. 2, Hongpi Honghua) were taken from the National Germplasm Changsha Ramie Garden, and DNA was extracted using the Tiangen kit. After the concentration of the extracted DNA is detected by electrophoresis, the concentration of the sample DNA is calculated and diluted to the required concentration.
[0062] 1.2.2 PCR using the designed SNP primers
[0063] PCR reaction system: 20μL reaction system composition is shown in Table 4
[0064] Table 4 Composition of PCR reaction system
[0065] System composition
Final concentration
Mg 2+
2.0mmol / L
Taq Buffer
1×
dNTP Mix
200μmol / L each
...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com