Method and kit for detecting HPV (Human Papillomavirus) genotype

Abstract

The invention discloses a method and a kit for detecting an HPV (Human Papillomavirus) genotype. The method for detecting the HPV genotype comprises the following steps of: A, extracting DNA (Deoxyribonucleic Acid); b, carrying out PCR (Polymerase Chain Reaction) on a DNA template, and amplifying to obtain a PCR product of an HPV-infected DNA sample; and C, carrying out restricted segment length polymorphism analysis through restriction endonuclease. The method is low in detection cost and can be used for rapidly and intuitively determining the type of the infected HPV according to a special HPV type atlas.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and a kit for detecting HPV genotypes. Background technique [0002] Cervical cancer is one of the most common cancers in women worldwide. There are approximately 500,000 cases of cervical cancer each year, of which nearly 275,000 patients die from the cancer. In 1976, German scholar zur Hausen discovered that human papilloma virus (HPV) plays a very important role in the pathogenesis of cervical cancer. Studies show that about 70 percent of HPV infections clear up on their own within 7 to 12 months of initial infection with the virus, and 90 percent clear within 2 years. The remaining 10% of HPV infections will develop into persistent infections. Persistent infection with a high-risk HPV genotype (HR-HPV) is a key risk factor in the development of cervical cancer. The onset of the cancer often lasts for as long as 10 years. Therefore, early detection of HPV genotype, e...

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a method and kit for detecting HPV genotypes, aiming at solving the problem of complicated operation process of the existing detection technology

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