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Primer-probe composition, kit and detection method for detecting seven kinds of hot-spot mutation of KRAS gene of humans
ActiveCN104805208AAccurate detectionHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationKRASTyping
The invention discloses a primer-probe composition for detecting seven kinds of hot-spot mutation of KRAS gene of humans. The primer-probe composition comprises 7 pairs of specific primers and one kind of specific probes, wherein the 7 pairs of specific primers are respectively prepared from 7 kinds of ARMS primers and one kind of downstream primer. The invention further discloses a kit containing the primer-probe composition, and a method for detecting mutation by using the kit. The primer-probe composition disclosed by the invention is high in sensitivity and specificity; when the kit is used, the detection processes are all reactions carried out in closed tubes, so that the pollution is significantly reduced, and besides, a UNG enzyme anti-pollution system and an internal control system are added, so that more accurate and stable typing detection can be performed on samples, and the results are guaranteed to be genuine and credible; the detection method is fast, the detection can be completed within 100 minutes, the result judgment is simple and objective, and the analysis is convenient.
Owner:SHANDONG VIGENE BIOSCI
Human SLCO1B1 and ApoE (apolipoprotein E) gene polymorphism detection kit
InactiveCN104846085AEasy to detectAccurate Typing DetectionMicrobiological testing/measurementApolipoprotein e4SLCO1B1
The invention provides a human SLCO1B1 and ApoE (apolipoprotein E) gene polymorphism detection kit, comprising a PCR (polymerase chain reaction) buffer solution, dNTP (deoxy-ribonucleoside triphosphate), MgCl2, four groups of specific primers, four groups of specific probes, an internal standard system, HotStart Taq enzyme and UNG (uracil-N-glycosylase) enzyme. The detection kit has the advantages of high specificity, high sensitivity, ease and quickness of operation, high throughput, safety, objectiveness of result interpretation, and the like.
Owner:WUHAN YZY MEDICAL SCI & TECH
Human face aging analogue method based on face super-resolution process
InactiveCN101425138AFast operationThe results are authenticCharacter and pattern recognitionPattern recognitionComputer graphics (images)
The invention discloses a human face aging simulating method based on human face super-resolution treatment. The method comprises the following steps: normalizing a human face image; training a super-resolution method for each age bracket; reducing the resolution of each inputted image; and performing the human face super-resolution treatment for an appointed age bracket, i.e. utilizing the trained human face super-resolution method to fill face venation information on the appointed age into the inputted face image with low-absolution so as to obtain a human face aging simulation image. The available human face super-resolution method is a human face super-resolution method based on learning. The invention adopts eigentransformation, can be applicable to any human face super-resolution methods based on the learning, utilizes the human face super-resolution based on the learning and can genuinely and believably simulate human face aging; and the invention only takes the change of human face venation, so the calculation is fast.
Owner:BEIHANG UNIV
Specific sequence specific primers-polymerase chain reaction primers and kit for human MTHFR and MTRR gene polymorphism detection
InactiveCN105002275AAccurate Typing DetectionStable Typing DetectionMicrobiological testing/measurementDNA/RNA fragmentationInternal standardPolymorphism Detection
The present invention relates to the fields of biotechnology and medicine, and provides specific sequence specific primers-polymerase chain reaction primer sequences and a kit for simultaneously detecting human MTHFR gene polymorphism and human MTRR gene polymorphism, wherein the kit contains the specific primers, specific probes, an internal standard system, Taq enzyme and UNG enzyme. According to the present invention, the primers and the kit have advantages of strong specificity, high sensitivity, simple and rapid operation, high-throughput, safety, objective result interpretation, and the like.
Owner:WUHAN YZY MEDICAL SCI & TECH
Fluorescent genotyping detection kit and detection method for eight K-ras gene mutations
InactiveCN103695555AReduce the number of reaction tubesFew samplesMicrobiological testing/measurementK-ras GenesFluorescence
The invention belongs to the field of biotechnology and clinical molecular diagnosis and in particular relates to a fluorescent genotyping detection kit and a detection method for eight human K-ras gene mutations. According to the method, an MGB (minor groove binder) probe technology is combined with a mismatch ARMS (amplification refractory mutation system) technology, an MGB probe complementary with a DNA (deoxyribonucleic acid) plus strand and an ARMS downstream primer complementary with a DNA minus strand are designed at allelic gene loci, a mismatch locus is introduced in the ARMS downstream primer, the MGB probe is overlapped with the ARMS primer by not more than 5 bp, and the eight human K-ras gene mutations are subjected to genotyping detection by four reaction pipes based on multiple fluorescent PCR (polymerase chain reaction). The method has the advantages of good specificity, high sensitivity, simplicity and quickness for operation, accuracy for genotyping, simplicity for result interpretation and the like, and can be used for detecting the clinical K-ras gene mutations and helping doctors screen the crowed with effective anti-EGFR (epidermal growth factor receptor) treatment.
Owner:SHANGHAI XINGYAO MED TECH DEV CO LTD +1
Photovoltaic assembly quality automatic detection method
InactiveCN103022219AThe results are authenticIncrease production capacitySemiconductor/solid-state device testing/measurementFinal product manufactureEconomic benefitsSoftware
The invention provides a photovoltaic assembly quality automatic detection method. The method comprises the following steps of: (A) carrying out assembly connection and electromotion; (B) filming picture through equipment; (C) carrying out picture analysis and comparison; (D) offering a judgment result through software; (E) preserving data and bar codes; and (F) finishing and entering the next circulation. The photovoltaic assembly quality automatic detection method provided by the invention effectively solves the problem of supposed judgment diversity due to standard diversification, and the detection method is safe, rapid and high efficient, the automaticity is high, and the economic benefit is improved and the like.
Owner:SUZHOU SUOLIWANG NEW ENERGY TECH CO LTD
Detection kit for human BRAF gene V600E mutation
InactiveCN105039514AAccurate detection of genetic mutationsReduce pollutionMicrobiological testing/measurementBraf genesLocked nucleic acid
The invention provides a detection kit and a detection method for human BRAF gene V600E mutation. The detection kit comprises a first reagent pack and a second reagent pack, wherein each of the first reagent pack and the second reagent pack comprises PCR buffer solution, dNTP, MgCl2, a specific probe, a locked nucleic acid (LNA) primer, an interior label system, HotStart Taq enzyme and UNG enzyme; the first reagent pack comprises a primer pair SEQ ID NO:1 and SEQ ID NO:2, and a probe SEQ ID NO:3; the second reagent pack comprises a primer pair SEQ ID NO:1 and SEQ ID NO:4, and a probe SEQ ID NO:5; and the SEQ ID NO:4 is LNA primer. The detection kit and the detection method can be used for detecting human BRAF gene V600E mutation, and has the advantages of strong specificity, high sensitivity, simplicity and rapidness in operation, high flux, safety, objective result determination and the like.
Owner:WUHAN YZY MEDICAL SCI & TECH
Human CYP2C9 and VKORC1 genetic polymorphism detection kit
InactiveCN105177116AEasy to detectAccurate Typing DetectionMicrobiological testing/measurementVKORC1Internal standard
The present invention provides a human CYP2C9 and VKORC1 genetic polymorphism detection kit. The kit includes a PCR buffer, dNTP, MgCl2, 2 groups of specific primers, 2 groups of specific probes, an internal standard system, HotStart Taq enzyme and UNG enzyme. The detection kit for detecting CYP2C9 and VKORC1 genetic polymorphism has the advantages of high specificity, high sensitivity, easy and fast operation, high-throughput, security, and objective interpretation of the results.
Owner:WUHAN YZY MEDICAL SCI & TECH
Consumer online shopping risk assessment method of multi-index projection decision method
The invention relates to a consumer online shopping risk assessment method of a multi-index projection decision method and belongs to the data processing technology field. The method comprises the following steps of establishing a consumer online shopping risk assessment index model from a consumer perspective, a seller, a third-party platform perspective and a product perspective; hierarchicallyclassifying the multiple evaluation index dimensions contained in the above three perspectives according to an evaluation index attribute to form a complete assessment decision configuration which includes multiple levels and reflects the system performance from different visual angle; and based on an analytical structure model, depicting the intricate connections among indexes, using an analytichierarchy process to endow corresponding weights to the indexes, and using an improved multi-index projection decision method to scientifically and effectively assess the risks of online shopping forconsumers. In the invention, the integrity, the scientificity and the validity of the indexes are clarified; and problems of insufficient consumer online shopping risk evaluation index system construction verification, the lack of an evaluation effect and case verification, and less weight evaluation in combination evaluation in the prior art are solved.
Owner:KUNMING UNIV OF SCI & TECH
Unexpected biological event field hazard evaluation simulation system
ActiveCN101894353ABig Hazard DepthLarge hazard breadthData processing applicationsEmergency treatmentHazard evaluation
The invention relates to an unexpected biological event field hazard evaluation simulation system, which comprises an information receiving module, a hazard preliminary evaluation module, a GPS module, an information storage module, a hazard calculation module, and a hazard display module. The information receiving module is used for receiving biological hazard data of a hazard field; the hazard preliminary evaluation module is used for calculating the maximum hazard depth, hazard width and hazard area of pathogenic microorganisms; the GPS module is used for positioning a geographical pathogenic microorganism-polluted area on a national administrative map; the information storage module is used for storing the national grid population data; the hazard calculation module is used for transferring the grid population data inside the geographical pathogenic microorganism-polluted area from the information storage module and calculating the number of infected people and ill and dead people after infection; and the hazard display module displays the geographical pathogenic microorganism-polluted positioned by the GPS module and the number of infected people and ill and dead people after infection calculated by the hazard calculation module on the national administrative map. The unexpected biological event field hazard evaluation simulation system can be widely applied to emergency treatment of a biological hazard attack event.
Owner:MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
MTHFR and MTRR gene polymorphism detection primer, probe, kit and application
InactiveCN110423801AAvoid the risk of cross-contaminationInhibit denaturation of substancesMicrobiological testing/measurementDNA/RNA fragmentationGenomic DNAMthfr c677t
The invention provides an MTHFR and MTRR gene polymorphism detection primer and probe combination, which includes an MTHFR C677T, MTHFR A1298C and MTRR A66G gene polymorphism detection primer and probe combination. The invention further provides a detection kit for the MTHFR and MTRR gene polymorphism. The kit is prepared from the following components of a nucleic acid releasing agent, 2*PCR reaction mixture (including a thermally activated Taq DNA polymerase and a UDG enzyme), three sets of specific primers, three sets of specific probes and an internal standard system. The kit only needs tobriefly treat a 2 [mu]l blood sample at the room temperature to produce high-quality DNA for repeated detection, the detection specificity is good, the coincidence rate with a gold standard sequencingmethod is 100%, the sensitivity is high, accurate typing of 0.2ng genomic DNA can be achieved, the detection process is short in consumed time, the process from sample processing to detection resultscan be completed within 1 hour.
Owner:北京协和洛克生物技术有限责任公司
Power grid key transmission section limit transmission capacity calculation method with consideration of artificial scheduling knowledge
ActiveCN103326356AConforms to the operating modeImprove adaptabilitySpecial data processing applicationsAc network circuit arrangementsPower gridLoad following power plant
A power grid key transmission section limit transmission capacity calculation method with consideration of artificial scheduling knowledge includes the steps of S 1) definition of an electricity generation area and a load area; S 2 definition of an electricity generation load increasing mode; S 3 calculation of key transmission section limit transmission capacity by the adoption of a continuous power flow mode. According to the power grid key transmission section limit transmission capacity calculation method with consideration of the artificial scheduling knowledge, the continuous power flow mode of an electricity generation area load area definition mode and the electricity generation load increasing mode with the consideration of the artificial scheduling knowledge is adopted to calculate the key transmission section limit transmission capacity, so the calculation result of the key transmission section limit transmission capacity is fitter for a power grid practical operational mode, adaptability of the key transmission section limit transmission capacity for power grid practical on-line operation is improved, and the result is more real and believable.
Owner:POWER DISPATCHING CONTROL CENT OF GUANGDONG POWER GRID CO LTD +1
Kit for detecting polymorphism of APOE gene and SLCO1B1 gene
InactiveCN108753951AEasy to operateAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationGeneticsFluorescent pcr
The invention provides a kit for detecting polymorphism of an APOE gene and an SLCO1B1 gene. The kit comprises primers used for detecting polymorphism sites T388C and C526T of the APOE gene and expressed as SEQ ID NO. 1-6, primers used for detecting polymorphism sites A388G and T521C of the SLCO1B1 gene and expressed as SEQ ID NO. 7-12, and internal quality control housekeeping gene primers expressed as SEQ ID NO. 13-20. The kit is used for carrying out fluorescence PCR by using ARMS-PCR and fluorescence fusion curve techniques, is capable of achieving accurate detection of one polymorphism oftwo genes in the same reaction tube, is high in sensitivity and high in specificity, and can meet detection of oral epithelial cells, dried-blood spots and whole-blood samples; the whole detection process is short in time; the medication basis can be provided for the doctor in the first time; the medication risks of the patients can be reduced.
Owner:SHENZHEN YOU SHENGKANG BIOSCI CO LTD
Method for measuring occurrence thickness of oil film in tight reservoir micro-nano pore throat
ActiveCN103759680ASolve the problem of thickness observation calculationThe results are authenticUsing wave/particle radiation meansThroatMicro nano
The embodiment of the invention provides a method for measuring the occurrence thickness of an oil film in a tight reservoir micro-nano pore throat. The method includes the steps of selecting a core sample; carrying out element content measuring on the movable oil film of the core sample through an energy disperse spectroscopy to obtain a mass percent and an atomicity mass percent of elements; according to the mass percent and the atomicity mass percent of the elements in the core sample, calculating a detection range V of the energy disperse spectroscopy and a percentage C of the size of the movable oil film in a total detection range of the energy disperse spectroscopy; measuring the size of a pore of oil film occurrence by means of a field emission-environment scanning electron microscope, wherein the length is x, the width is y, the height is z, and calculating the coverage surface area S of the oil film according to a formula defined as S=phi(z2+(MAX(x,y) / 2)2); calculating the occurrence thickness of the oil film by means of the formula defined as H=CV / S. By means of a high-power observation and energy spectrum quantitative determination method, the problem that resolution of a conventional method can not meet the requirement of observation and calculation of the crude oil thickness in the micro-nano pore throat, and solves the difficult problem that oil in the core sample is volatile and can not be observed easily, and results are genuine and believable.
Owner:PETROCHINA CO LTD
Method and kit for detecting ADRB1 gene single nucleotide polymorphic site
InactiveCN106834466AHigh sensitivityStrong specificityMicrobiological testing/measurementAgainst vector-borne diseasesFluorescent quenchingGenome
The invention belongs to the technical field of molecular biology, and particularly relates to a method for detecting an ADRB1 gene single nucleotide polymorphic site. The method comprises the following specific steps: 1) design of a specific primer: designing a specific amplification primer, an ARMS specific amplification primer, an interior label amplification primer, a specific probe and an interior label probe according to a proved ADRB1 gene label single nucleotide polymorphic site, wherein the 5' end of the specific probe carries a fluorescent group, and the 3' end of the specific probe carries a fluorescent quenching group; 2) acquiring DNA (Deoxyribonucleic Acid) of a sample genome to be detected; 3) fluorescent quantitative PCR (Polymerase Chain Reaction) amplification: performing a PCR reaction by taking the DNA of a sample to be detected as a template with the specific amplification primer in the step 1), and analyzing a detection result. The method has high sensitivity and high specificity, and is easy and convenient to operate; detection can be finished within 90 minutes, and a result interpretation method is simple, objective and convenient for analysis.
Owner:重庆迪威纳生物技术有限公司
Method for determining return period of chlorophyll a
InactiveCN102508996AForecast return periodReflect relevanceSpecial data processing applicationsChlorophyll aEutrophication
The invention discloses a method for determining a return period of chlorophyll a. The joint distribution of the chlorophyll a and environment variables is constructed by Copula functions to research the relevance between the chlorophyll a and the environment variables so as to determine the return period of the chlorophyll a. According to the method, the relevance between the chlorophyll a and environmental factors is researched by a Copula function method, the relativity between the chlorophyll a and the environmental factors can be determined through Kendall 'st, and the joint distribution of the chlorophyll a and the environmental factors can be acquired under the conditions of not assuming edge distribution types, so that the return period of the chlorophyll a is predicted under the condition of different environment variable changes, and a scientific decision-making basis for lake eutrophication management is provided.
Owner:NANJING UNIV
Method for efficiently screening high linolenic acid germplasm of brassica napus
PendingCN111528088AImprove traitsImprove breeding valuePlant genotype modificationBiotechnologyBrassica
The invention discloses a method for efficiently screening high linolenic acid germplasm of brassica napus. The method comprises the following steps of (1) expanding phenotypic variation of linolenicacid by utilizing a plurality of different ecological environments in winter and spring; (2) establishing different screening indexes according to different screening environments and generations; (3)performing comprehensive screening on the linolenic acid yield and the fatty acid composition to quickly obtain the high linolenic acid germplasm; (4) screening agronomic and quality characters whilescreening linolenic acid by utilizing a method of combining selfing and plant selection, so as to improve the comprehensive characters of the high linolenic acid germplasm; and (5) performing geneticstability analysis and quantitative evaluation on the many-year and multi-point results, so as to obtain the genetically stable high linolenic acid germplasm of brassica napus. The method disclosed by the invention has the characteristics of quantifiability and mechanical repeatability, can meet the screening requirements of the high linolenic acid germplasm of brassica napus, and can also enrichand complete the brassica napus breeding method; and meanwhile, beneficial reference can be provided for the development of fatty acid improvement work of other crops.
Owner:陕西省杂交油菜研究中心
Primer set for detecting mutation of ESR1 gene, reagent, kit and method thereof
PendingCN110452988AAccurate detectionMeet testing needsMicrobiological testing/measurementDNA/RNA fragmentationPlasma samplesTissue sample
The invention discloses a primer set for detecting mutation of an ESR1 gene, a reagent, a kit and a method thereof, which relate to the field of biological medical science. The primer set for detecting the mutation of the ESR1 gene disclosed in the present invention includes any one or more of the following primer combinations: a primer combination 1, a primer combination 2, and a primer combination 3. The primer set can accurately detect 11 mutations of the ESR1 gene, and has the advantages of high sensitivity and good specificity, and can accurately detect the mutation of 0.1% in a total of20 ng DNA samples. The primer set can be applied to the detection of tissue samples and plasma samples, and can flexibly adapt to the needs of clinical testing. The detection method is simple in operation and the detection results are clear and easy to analyze.
Owner:WUHAN YZY MEDICAL SCI & TECH
Reliability testing device for ablation-resistant materials
InactiveCN110985242ADetection reliabilityEasy to detectRocket engine plantsCombustion chamberInsulation layer
The invention discloses a reliability testing device for ablation-resistant materials, and relates to the technical field of reliability detection. Ablation impact detection on ablation-resistant heat-insulation materials for a short time and with a variable load is mainly carried out. The reliability testing device for the ablation-resistant materials is mainly composed of three components of a combustion chamber, a spray pipe seat and spray pipes, high-temperature and high-pressure gas generated in the combustion chamber is used for turning by 90 degrees in the spray pipe seat to ablate andimpact the ablation-resistant materials, and by replacing the spray pipes with different throat diameters, the reliability of the ablation-resistant materials under the combined effect of different pressure and temperature can be simulated. The reliability testing device has a simple result and can perform ablation impact detection on test pieces in a short time, and the device is internally coated with a heat insulation layer, so that the service life of the reliability testing device is greatly guaranteed; and in addition, the types and sizes of propellants used in the combustion chamber aredifferent, the ablation impact detection under different working pressure and time can be simulated, more comprehensive testing can be carried out on the test pieces, and the efficiency of the reliability test of the ablation-resistant materials is greatly improved.
Owner:NANJING UNIV OF SCI & TECH
Network source coordination performance test method and system thereof
InactiveCN105866682AThe results are authenticReduce manual interventionDynamo-electric machine testingMicrocomputerDigital signal processing
A network source coordination performance testing method and system, including an upper platform (1) composed of an embedded industrial computer and its peripherals, an intermediate platform (2) composed of a high-speed digital signal processor and its peripherals, a power amplifier and its The underlying platform composed of protection circuits (3). The upper platform uses a high-performance embedded industrial computer as a control microcomputer, and peripherals include a host (4) and a slave (5); communicate with the middle control board through a PCI interface, and perform wireless communication with the slave through a PCI interface. The intermediate platform takes the high-speed digital signal processor DSP56F807 as the core, and has multiple I / O ports and various peripheral devices. The bottom platform uses a high-power high-fidelity modular power device as a power output stage; the bottom platform is designed with a voltage power amplifier circuit and a current power amplifier circuit. The test method of the present invention can verify the coordination performance of the network source by taking advantage of the unit shutdown or maintenance opportunities, and can complete all tests within 15 minutes without the intervention of test personnel.
Owner:ELECTRIC POWER RES INST STATE GRID JIANGXI ELECTRIC POWER CO +1
A kit and method for extracting whole genome dna from blood
ActiveCN104017800BNo pollution in the processReduce the chance of secondary pollutionDNA preparationWhite blood cellNucleotide
Owner:ZICHENG RUISHENGHUI BEIJING BIOTECH DEV CO LTD
Kit used for detecting human MTHFR and MTRR gene polymorphism, and detection method thereof
InactiveCN108998518AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationTrue positive rateGene
The invention provides a kit used for detecting human MTHFR and MTRR gene polymorphism, and a detection method thereof. The kit is used for avoiding defects and insufficient in conventional gene polymorphism detection technology. A primer and a probe used for detecting MTHFR and MTRR gene polymorphism are provided; the primer and the probe can be used for detecting MTHFR gene 677C>T and 1298A>C site and MTRR gene 66A>G site polymorphism, and are high in sensitivity and specificity. The kit can be used for rapid, high efficiency, and accurate detection of human MTHFR gene 677C>T and 1298A>C site and MTRR gene 66A>G site polymorphism. The invention also provides the detection method used for detecting human MTHFR and MTRR gene polymorphism using the kit. The detection method is convenient inoperation, high in sensitivity and specificity, wide in suitable machine type range, real and reliable in detection results, and is convenient for popularization.
Owner:SHUWEN BIOTECH CO LTD
Method for detecting codon pair bias of bovine whole-genome
InactiveCN102693368AThe results are authenticSpecial data processing applicationsSingle sequenceGenome
The invention discloses a method for detecting codon pair bias of a bovine whole-genome. The method comprises the steps of: calculating codon pair score CPS of 3721 kinds of codon pairs in a CDS (coding sequence)of the bovine whole-genome; analyzing codon pair bias CPB of a single sequence in the bovine whole-genome according to the CPS of different codon pairs, wherein a CPB value of a sequence is an arithmetic average value of values of all codon pairs in the sequence; establishing a codon pair bias profile for each CDS in the genome according to the CPS of 3721 kinds of codon pairs in the genome and an arranging sequence of the codon pairs in the CDS ; aligning codon pair bias profiles of all CDS in the bovine genome form a 5' end and a 3' end of the sequence, and calculating an average value of the CPS of each codon pair locus in alignment results, thereby obtaining a whole-genome averaged codon pair bias profile of all CDS of the bovine.
Owner:NORTHWEST A & F UNIV
High-sensitivity gene mutation detection method and kit used in same
InactiveCN106755489AAccurate detectionHigh sensitivityMicrobiological testing/measurementExonuclease IWild type
The invention discloses a high-sensitivity gene mutation detection kit which comprises a PCR buffer solution, DNA polymerase without exonuclease activity, an LNA modified ARMS-like primer, a conventional primer, dNTP mixed liquid, nucleic acid dye and reference dye. The end 3' of the LNA modified ARMS-like primer is nucleic acid analogue; methylene is introduced to the sites of 2' oxygen atom and 4' carbon atom of the carbon ring of the nucleic acid analogue to form a lock structure, so that the LNA remarkably enhances the oligonucleotide identifying capability. The invention also provides a high-sensitivity gene mutation detection method adopting the kit, wherein if the result of PCR reaction has an amplification curve, the to-be-detected sample is a mutant sample; otherwise, if the result of PCR reaction does not have an amplification curve, the to-be-detected sample is a wild sample.
Owner:HANGZHOU JINXI BIOLOGICAL TECH
Primer, probe, kit and detection method for detecting ESR1 gene expression
InactiveCN112646864ASmall sample sizeAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationReference genesMedicine
The invention discloses a primer pair combination for detecting ESR1 gene expression, and belongs to the technical field of medical molecular biology. The primer pair combination comprises a first primer pair for amplifying an ESR1 gene, and further comprises a second primer pair and a third primer pair which are used for amplifying reference genes ACTB and PGK1 respectively. The invention further discloses a probe combination which comprises a first probe for targeting an amplification product of the first primer pair, and further comprises a second probe for targeting an amplification product of the second primer pair and a third probe for targeting an amplification product of the third primer pair. The invention further discloses a kit prepared by utilizing the primer pair combination or the primer pair combination and the probe combination, and a method for detecting the ESR1 gene. When the ESR1 gene expression is detected by utilizing the primer pair combination, the sample type is not limited, the detection result is more accurate, the detection period is short, the human error is small, the repeatability is good, and the primer pair combination is more sensitive and reliable compared with IHC.
Owner:杭州联川基因诊断技术有限公司
Fishery habitat water quality evaluation method and system
ActiveCN111126768AImprove accuracyFast automatic calculationDigital data information retrievalGeneral water supply conservationEvaluation resultWater quality
The invention discloses a fishery habitat water quality evaluation method which comprises the following steps: constructing a water quality prior probability table, a copepod density level table and acopepod density conditional probability table of an evaluation object according to historical water quality monitoring data and historical copepod density monitoring data of the evaluation object; collecting the current copepod density of the evaluation object, and constructing a density set; searching a copepod density grade table and a copepod density conditional probability table, and obtaining a copepod density conditional probability corresponding to each copepod density grade in the density set; substituting the copepod density conditional probability and the water quality prior probability into a Bayesian inference model, sequentially calculating the water quality posterior probability corresponding to each copepod density in the density set, and taking the water quality posteriorprobability corresponding to the last copepod density as an evaluation result. The invention further discloses a corresponding system. Only the copepod density is collected, the water quality evaluation result can be rapidly obtained based on the copepod density, and multiple physicochemical indexes are not needed.
Owner:HOHAI UNIV
Database attribute sensitivity quantification method based on attack probability
ActiveCN111191291AThe results are authenticPreserve data availabilityDigital data protectionAlgorithmAttack
The invention discloses an attack probability-based database attribute sensitivity quantification method, which comprises the following steps of: 1) endowing each column in a database with the probability of possibly obtaining the column in advance by an attacker; 2) inputting the database into a database primary key analysis system to obtain all primary keys and composite primary keys of the database; 3) sorting each column in the database according to a result output in the step 2), and finding out which primary keys and composite primary keys each column appears in; 4) calculating the probability that each column in the database is successfully attacked; and 5) performing sensitivity quantification and sorting on each column in the database according to the probability calculated in thestep 4 that each column in the database is successfully attacked, outputting sensitivity quantification and sorting results, and completing database attribute sensitivity quantification based on attack probability. According to the method, sensitivity quantification and sorting can be carried out on all attributes in the database according to the attack success probability of an attacker, and theaccuracy is high.
Owner:XIDIAN UNIV
Drug component detection method and system based on data analysis
ActiveCN114112980AHigh precisionThe results are authenticMaterial analysis by optical meansProtein targetPharmaceutical drug
The invention provides a drug component detection method and system based on data analysis, and the method comprises the steps: obtaining a first target protein, a to-be-detected target drug and a second target protein after the target drug acts on the first target protein, and extracting functional group data through a terahertz time-domain spectroscopy technology, relevant data of targeted proteins and targeted drugs are obtained and input into a generative model to generate a result, and the result is corrected by adopting a discrimination network. The method disclosed by the invention has the beneficial effects that not only is chromatographic analysis carried out on the targeted drug, but also comprehensive analysis is carried out from data after the targeted drug reacts with the targeted protein, so that the accuracy of result analysis is improved, and the result is more real and credible.
Owner:武汉宏韧生物医药股份有限公司
Human TGFBI gene mutation detection kit and detection method thereof
PendingCN109161589AStrong specificityHigh sensitivityMicrobiological testing/measurementDiseaseTGFBI gene
The invention provides a human TGFBI gene mutation detection kit and a detection method thereof. The invention provides effective and targeted protection at an early stage for carriers of a corneal abnormality mutation gene, and can delay the development of a disease course and even avoid the onset of a disease. The invention provides a primer and a probe for detecting corneal dystrophy caused bymutation of a human TGFBI gene at a 124-site and a 555-site, and the primer and the probe are capable of detecting the mutation of the TGFBI gene at the 124-site and the 555-site, high in sensitivityand strong in specificity. The invention provides the corneal abnormality mutation gene detection kit which is capable of rapidly, efficiently and accurately detecting the mutation of the human TGFBIgene at the 124-site and the 555-site. The invention further provides the detection method for detecting the mutation of the human TGFBI gene at the 124-site and the 555-site by the kit, and the detection method has the advantages of simple operation, high sensitivity, high specificity, wide application models, true and reliable detection results, easy promotion and the like.
Owner:SHUWEN BIOTECH CO LTD
Detection primers, probes and detection kits for human alk gene expression
ActiveCN106978498BEasy to readHigh detection sensitivityMicrobiological testing/measurementDNA/RNA fragmentationWild typeAnaplastic lymphoma kinase
The invention relates to the field of biotechnologies and medicines and in particular discloses a detection primer, a probe and a detection kit for human ALK (Anaplastic Lymphoma Kinase) gene expression. The detection primer comprises sequences shown as SEQ ID NO: 1 to SEQ ID NO: 8; the sequence of the probe is shown as SEQ ID NO: 9 to SEQ ID NO: 12; a 5' end of the probe is modified by FAM or VIC and a 3' end of the probe is modified by NFQ-MGB. The primer and the probe, provided by the invention, can be used for determining whether ALK gene fusion exists or not through detecting expression difference of the 5' end and 3' end of an ALK gene. The kit contains the detection primer and the probe, and a quality control system and the like. The detection primer, the probe and the detection kit can be used for detecting mutation which is as low as 5 copies and can tolerate reverse transcripts of 600ng of genome DNA (Deoxyribonucleic Acid) and 5mu g of wild RNA (Ribonucleic Acid); a whole reverse transcription PCR (Polymerase Chain Reaction) and fluorescent PCR detection process can be finished within only 80min.
Owner:WUHAN YZY MEDICAL SCI & TECH
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