51results about How to "The results are authentic" patented technology

The invention discloses a primer-probe composition for detecting seven kinds of hot-spot mutation of KRAS gene of humans. The primer-probe composition comprises 7 pairs of specific primers and one kind of specific probes, wherein the 7 pairs of specific primers are respectively prepared from 7 kinds of ARMS primers and one kind of downstream primer. The invention further discloses a kit containing the primer-probe composition, and a method for detecting mutation by using the kit. The primer-probe composition disclosed by the invention is high in sensitivity and specificity; when the kit is used, the detection processes are all reactions carried out in closed tubes, so that the pollution is significantly reduced, and besides, a UNG enzyme anti-pollution system and an internal control system are added, so that more accurate and stable typing detection can be performed on samples, and the results are guaranteed to be genuine and credible; the detection method is fast, the detection can be completed within 100 minutes, the result judgment is simple and objective, and the analysis is convenient.

The invention provides a human SLCO1B1 and ApoE (apolipoprotein E) gene polymorphism detection kit, comprising a PCR (polymerase chain reaction) buffer solution, dNTP (deoxy-ribonucleoside triphosphate), MgCl2, four groups of specific primers, four groups of specific probes, an internal standard system, HotStart Taq enzyme and UNG (uracil-N-glycosylase) enzyme. The detection kit has the advantages of high specificity, high sensitivity, ease and quickness of operation, high throughput, safety, objectiveness of result interpretation, and the like.

The invention belongs to the field of biotechnology and clinical molecular diagnosis and in particular relates to a fluorescent genotyping detection kit and a detection method for eight human K-ras gene mutations. According to the method, an MGB (minor groove binder) probe technology is combined with a mismatch ARMS (amplification refractory mutation system) technology, an MGB probe complementary with a DNA (deoxyribonucleic acid) plus strand and an ARMS downstream primer complementary with a DNA minus strand are designed at allelic gene loci, a mismatch locus is introduced in the ARMS downstream primer, the MGB probe is overlapped with the ARMS primer by not more than 5 bp, and the eight human K-ras gene mutations are subjected to genotyping detection by four reaction pipes based on multiple fluorescent PCR (polymerase chain reaction). The method has the advantages of good specificity, high sensitivity, simplicity and quickness for operation, accuracy for genotyping, simplicity for result interpretation and the like, and can be used for detecting the clinical K-ras gene mutations and helping doctors screen the crowed with effective anti-EGFR (epidermal growth factor receptor) treatment.

The present invention provides a human CYP2C9 and VKORC1 genetic polymorphism detection kit. The kit includes a PCR buffer, dNTP, MgCl2, 2 groups of specific primers, 2 groups of specific probes, an internal standard system, HotStart Taq enzyme and UNG enzyme. The detection kit for detecting CYP2C9 and VKORC1 genetic polymorphism has the advantages of high specificity, high sensitivity, easy and fast operation, high-throughput, security, and objective interpretation of the results.

The invention relates to a consumer online shopping risk assessment method of a multi-index projection decision method and belongs to the data processing technology field. The method comprises the following steps of establishing a consumer online shopping risk assessment index model from a consumer perspective, a seller, a third-party platform perspective and a product perspective; hierarchicallyclassifying the multiple evaluation index dimensions contained in the above three perspectives according to an evaluation index attribute to form a complete assessment decision configuration which includes multiple levels and reflects the system performance from different visual angle; and based on an analytical structure model, depicting the intricate connections among indexes, using an analytichierarchy process to endow corresponding weights to the indexes, and using an improved multi-index projection decision method to scientifically and effectively assess the risks of online shopping forconsumers. In the invention, the integrity, the scientificity and the validity of the indexes are clarified; and problems of insufficient consumer online shopping risk evaluation index system construction verification, the lack of an evaluation effect and case verification, and less weight evaluation in combination evaluation in the prior art are solved.

A power grid key transmission section limit transmission capacity calculation method with consideration of artificial scheduling knowledge includes the steps of S 1) definition of an electricity generation area and a load area; S 2 definition of an electricity generation load increasing mode; S 3 calculation of key transmission section limit transmission capacity by the adoption of a continuous power flow mode. According to the power grid key transmission section limit transmission capacity calculation method with consideration of the artificial scheduling knowledge, the continuous power flow mode of an electricity generation area load area definition mode and the electricity generation load increasing mode with the consideration of the artificial scheduling knowledge is adopted to calculate the key transmission section limit transmission capacity, so the calculation result of the key transmission section limit transmission capacity is fitter for a power grid practical operational mode, adaptability of the key transmission section limit transmission capacity for power grid practical on-line operation is improved, and the result is more real and believable.

The invention belongs to the technical field of molecular biology, and particularly relates to a method for detecting an ADRB1 gene single nucleotide polymorphic site. The method comprises the following specific steps: 1) design of a specific primer: designing a specific amplification primer, an ARMS specific amplification primer, an interior label amplification primer, a specific probe and an interior label probe according to a proved ADRB1 gene label single nucleotide polymorphic site, wherein the 5' end of the specific probe carries a fluorescent group, and the 3' end of the specific probe carries a fluorescent quenching group; 2) acquiring DNA (Deoxyribonucleic Acid) of a sample genome to be detected; 3) fluorescent quantitative PCR (Polymerase Chain Reaction) amplification: performing a PCR reaction by taking the DNA of a sample to be detected as a template with the specific amplification primer in the step 1), and analyzing a detection result. The method has high sensitivity and high specificity, and is easy and convenient to operate; detection can be finished within 90 minutes, and a result interpretation method is simple, objective and convenient for analysis.

The invention discloses a method for determining a return period of chlorophyll a. The joint distribution of the chlorophyll a and environment variables is constructed by Copula functions to research the relevance between the chlorophyll a and the environment variables so as to determine the return period of the chlorophyll a. According to the method, the relevance between the chlorophyll a and environmental factors is researched by a Copula function method, the relativity between the chlorophyll a and the environmental factors can be determined through Kendall 'st, and the joint distribution of the chlorophyll a and the environmental factors can be acquired under the conditions of not assuming edge distribution types, so that the return period of the chlorophyll a is predicted under the condition of different environment variable changes, and a scientific decision-making basis for lake eutrophication management is provided.

The invention discloses a method for efficiently screening high linolenic acid germplasm of brassica napus. The method comprises the following steps of (1) expanding phenotypic variation of linolenicacid by utilizing a plurality of different ecological environments in winter and spring; (2) establishing different screening indexes according to different screening environments and generations; (3)performing comprehensive screening on the linolenic acid yield and the fatty acid composition to quickly obtain the high linolenic acid germplasm; (4) screening agronomic and quality characters whilescreening linolenic acid by utilizing a method of combining selfing and plant selection, so as to improve the comprehensive characters of the high linolenic acid germplasm; and (5) performing geneticstability analysis and quantitative evaluation on the many-year and multi-point results, so as to obtain the genetically stable high linolenic acid germplasm of brassica napus. The method disclosed by the invention has the characteristics of quantifiability and mechanical repeatability, can meet the screening requirements of the high linolenic acid germplasm of brassica napus, and can also enrichand complete the brassica napus breeding method; and meanwhile, beneficial reference can be provided for the development of fatty acid improvement work of other crops.

The invention discloses a primer set for detecting mutation of an ESR1 gene, a reagent, a kit and a method thereof, which relate to the field of biological medical science. The primer set for detecting the mutation of the ESR1 gene disclosed in the present invention includes any one or more of the following primer combinations: a primer combination 1, a primer combination 2, and a primer combination 3. The primer set can accurately detect 11 mutations of the ESR1 gene, and has the advantages of high sensitivity and good specificity, and can accurately detect the mutation of 0.1% in a total of20 ng DNA samples. The primer set can be applied to the detection of tissue samples and plasma samples, and can flexibly adapt to the needs of clinical testing. The detection method is simple in operation and the detection results are clear and easy to analyze.

The invention discloses a reliability testing device for ablation-resistant materials, and relates to the technical field of reliability detection. Ablation impact detection on ablation-resistant heat-insulation materials for a short time and with a variable load is mainly carried out. The reliability testing device for the ablation-resistant materials is mainly composed of three components of a combustion chamber, a spray pipe seat and spray pipes, high-temperature and high-pressure gas generated in the combustion chamber is used for turning by 90 degrees in the spray pipe seat to ablate andimpact the ablation-resistant materials, and by replacing the spray pipes with different throat diameters, the reliability of the ablation-resistant materials under the combined effect of different pressure and temperature can be simulated. The reliability testing device has a simple result and can perform ablation impact detection on test pieces in a short time, and the device is internally coated with a heat insulation layer, so that the service life of the reliability testing device is greatly guaranteed; and in addition, the types and sizes of propellants used in the combustion chamber aredifferent, the ablation impact detection under different working pressure and time can be simulated, more comprehensive testing can be carried out on the test pieces, and the efficiency of the reliability test of the ablation-resistant materials is greatly improved.

A network source coordination performance testing method and system, including an upper platform (1) composed of an embedded industrial computer and its peripherals, an intermediate platform (2) composed of a high-speed digital signal processor and its peripherals, a power amplifier and its The underlying platform composed of protection circuits (3). The upper platform uses a high-performance embedded industrial computer as a control microcomputer, and peripherals include a host (4) and a slave (5); communicate with the middle control board through a PCI interface, and perform wireless communication with the slave through a PCI interface. The intermediate platform takes the high-speed digital signal processor DSP56F807 as the core, and has multiple I/O ports and various peripheral devices. The bottom platform uses a high-power high-fidelity modular power device as a power output stage; the bottom platform is designed with a voltage power amplifier circuit and a current power amplifier circuit. The test method of the present invention can verify the coordination performance of the network source by taking advantage of the unit shutdown or maintenance opportunities, and can complete all tests within 15 minutes without the intervention of test personnel.

The invention provides a kit used for detecting human MTHFR and MTRR gene polymorphism, and a detection method thereof. The kit is used for avoiding defects and insufficient in conventional gene polymorphism detection technology. A primer and a probe used for detecting MTHFR and MTRR gene polymorphism are provided; the primer and the probe can be used for detecting MTHFR gene 677C>T and 1298A>C site and MTRR gene 66A>G site polymorphism, and are high in sensitivity and specificity. The kit can be used for rapid, high efficiency, and accurate detection of human MTHFR gene 677C>T and 1298A>C site and MTRR gene 66A>G site polymorphism. The invention also provides the detection method used for detecting human MTHFR and MTRR gene polymorphism using the kit. The detection method is convenient inoperation, high in sensitivity and specificity, wide in suitable machine type range, real and reliable in detection results, and is convenient for popularization.

The invention discloses a method for detecting codon pair bias of a bovine whole-genome. The method comprises the steps of: calculating codon pair score CPS of 3721 kinds of codon pairs in a CDS (coding sequence)of the bovine whole-genome; analyzing codon pair bias CPB of a single sequence in the bovine whole-genome according to the CPS of different codon pairs, wherein a CPB value of a sequence is an arithmetic average value of values of all codon pairs in the sequence; establishing a codon pair bias profile for each CDS in the genome according to the CPS of 3721 kinds of codon pairs in the genome and an arranging sequence of the codon pairs in the CDS ; aligning codon pair bias profiles of all CDS in the bovine genome form a 5' end and a 3' end of the sequence, and calculating an average value of the CPS of each codon pair locus in alignment results, thereby obtaining a whole-genome averaged codon pair bias profile of all CDS of the bovine.

The invention discloses a high-sensitivity gene mutation detection kit which comprises a PCR buffer solution, DNA polymerase without exonuclease activity, an LNA modified ARMS-like primer, a conventional primer, dNTP mixed liquid, nucleic acid dye and reference dye. The end 3' of the LNA modified ARMS-like primer is nucleic acid analogue; methylene is introduced to the sites of 2' oxygen atom and 4' carbon atom of the carbon ring of the nucleic acid analogue to form a lock structure, so that the LNA remarkably enhances the oligonucleotide identifying capability. The invention also provides a high-sensitivity gene mutation detection method adopting the kit, wherein if the result of PCR reaction has an amplification curve, the to-be-detected sample is a mutant sample; otherwise, if the result of PCR reaction does not have an amplification curve, the to-be-detected sample is a wild sample.

The invention discloses a primer pair combination for detecting ESR1 gene expression, and belongs to the technical field of medical molecular biology. The primer pair combination comprises a first primer pair for amplifying an ESR1 gene, and further comprises a second primer pair and a third primer pair which are used for amplifying reference genes ACTB and PGK1 respectively. The invention further discloses a probe combination which comprises a first probe for targeting an amplification product of the first primer pair, and further comprises a second probe for targeting an amplification product of the second primer pair and a third probe for targeting an amplification product of the third primer pair. The invention further discloses a kit prepared by utilizing the primer pair combination or the primer pair combination and the probe combination, and a method for detecting the ESR1 gene. When the ESR1 gene expression is detected by utilizing the primer pair combination, the sample type is not limited, the detection result is more accurate, the detection period is short, the human error is small, the repeatability is good, and the primer pair combination is more sensitive and reliable compared with IHC.

The invention discloses an attack probability-based database attribute sensitivity quantification method, which comprises the following steps of: 1) endowing each column in a database with the probability of possibly obtaining the column in advance by an attacker; 2) inputting the database into a database primary key analysis system to obtain all primary keys and composite primary keys of the database; 3) sorting each column in the database according to a result output in the step 2), and finding out which primary keys and composite primary keys each column appears in; 4) calculating the probability that each column in the database is successfully attacked; and 5) performing sensitivity quantification and sorting on each column in the database according to the probability calculated in thestep 4 that each column in the database is successfully attacked, outputting sensitivity quantification and sorting results, and completing database attribute sensitivity quantification based on attack probability. According to the method, sensitivity quantification and sorting can be carried out on all attributes in the database according to the attack success probability of an attacker, and theaccuracy is high.

The invention provides a human TGFBI gene mutation detection kit and a detection method thereof. The invention provides effective and targeted protection at an early stage for carriers of a corneal abnormality mutation gene, and can delay the development of a disease course and even avoid the onset of a disease. The invention provides a primer and a probe for detecting corneal dystrophy caused bymutation of a human TGFBI gene at a 124-site and a 555-site, and the primer and the probe are capable of detecting the mutation of the TGFBI gene at the 124-site and the 555-site, high in sensitivityand strong in specificity. The invention provides the corneal abnormality mutation gene detection kit which is capable of rapidly, efficiently and accurately detecting the mutation of the human TGFBIgene at the 124-site and the 555-site. The invention further provides the detection method for detecting the mutation of the human TGFBI gene at the 124-site and the 555-site by the kit, and the detection method has the advantages of simple operation, high sensitivity, high specificity, wide application models, true and reliable detection results, easy promotion and the like.