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Primer combination, kit and method for guiding detection of pravastatin individualized drug use related genes

A technology for pravastatin and gene detection, which is applied in the field of gene detection related to guiding the individualized medication of pravastatin drugs, can solve the problems of adverse reactions of clinical medication, lack of kit products, etc., and achieves reduction of medical costs, rapid detection, and interpretation of results. simple effect

Inactive Publication Date: 2019-09-06
南京派森诺基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problems of traditional clinical experience medical diagnosis and pravastatin drug administration, which are prone to clinical adverse reactions and there is no suitable kit product

Method used

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  • Primer combination, kit and method for guiding detection of pravastatin individualized drug use related genes
  • Primer combination, kit and method for guiding detection of pravastatin individualized drug use related genes
  • Primer combination, kit and method for guiding detection of pravastatin individualized drug use related genes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1: the preparation of kit

[0082] 1. Design and synthesis of primers and probes

[0083] For the SLCO1B1 (rs4149015), HMGCR (rs17244841), LDLR (rs1433099) gene loci in the human genome (for the sequence, refer to the human genome sequence published in the NCBI database), specific primers and primers were designed using Primer Premier 5.0 and Primer Express 3.0 software, respectively. Taqman probe.

[0084] Specific primers and probe sequences are shown in Table 1 below:

[0085] Table 1 Primer and probe sequence and length information

[0086]

[0087]

[0088] 2. Selection of reference substance

[0089] The positive control substances are SLCO1B1 (rs4149015), HMGCR (rs17244841), and LDLR (rs1433099) wild-type plasmids respectively;

[0090] The two positive controls are plasmids heterozygous for SLCO1B1 (rs4149015), HMGCR (rs17244841), and LDLR (rs1433099) gene loci wild type and mutant type in a quantitative ratio of 1:1;

[0091] The three pos...

Embodiment 2

[0105] Example 2: Using the above-mentioned kit to carry out the method for the detection of genes related to the individualized drug use of pravastatin

[0106] 1. Biological samples

[0107] The biological materials used in the present invention are all from Parsonuo Medical Laboratory. Anticoagulant blood was used for the remaining 100 cases tested in Paisino Medical Laboratory during September-December 2018.

[0108] 2. Take the DNA extracted from the sample to be tested as a PCR template:

[0109] The DNA extraction kit is the extraction kit "Blood Genomic DNA Extraction Kit" produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. (Cat. No.: DP318).

[0110] 1) Take 400ul of whole blood, add 800ul of cell lysate CL, mix well, centrifuge at 10000rpm / 11500×g for 1min, discard the supernatant, and repeat once if the lysis is not complete.

[0111] 2) Add 200ul buffer GS to the precipitation, mix well, then add 20ul proteinase K, mix well.

[0112] 3) Add 200ul buf...

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Abstract

The invention discloses a combination of primers for guiding detection of a pravastatin individualized drug use related genes, and is characterized in that the combination includes specific primers and probes for SLCO1B1 (rs4149015), HMGCR (rs17244841) and LDLR (rs1433099) gene loci. The invention also discloses a kit and a method. The kit has the advantages of simple operation, economy, fast detection, high accuracy, good specificity and simple result interpretation, and realizes rapid and accurate determination of polymorphisms of the pravastatin individualized drug use related genes, so asto reduce adverse reactions of common drug use of pravastatin in clinic, reduce medical cost and save social resources.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a primer combination, a kit and a method for guiding the detection of genes related to individualized pravastatin medication. Background technique [0002] Hyperlipidemia is a common metabolic disease in clinical practice. It is a disease in which one or more lipids in plasma are higher than the normal index due to abnormal fat metabolism or turnover. Hyperlipidemia is a chronic disease with high blood lipid levels. The etiology of hyperlipidemia is complex, and age, sex, heredity, environment and bad living habits can all lead to the occurrence of the disease. Clinical studies have shown that hyperlipidemia is a predisposing factor for various cardiovascular diseases, such as atherosclerosis and coronary heart disease. In recent years, with the continuous improvement of people's material life, the incidence of this disease has also increased year by year. At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 杜晴晴赵艳伟宣涛刘颖孙子奎
Owner 南京派森诺基因科技有限公司
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