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Kit and method for simultaneously and rapidly detecting SLCO1B1 and ApoE gene polymorphism

A technology of SLCO1B1521T and gene polymorphism, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of low specificity, high sample processing requirements, high cost, etc., and reduce operation steps and operation time Effect

Inactive Publication Date: 2019-03-12
江苏美因康生物科技有限公司
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Problems solved by technology

[0006] The main purpose of the present invention is to provide a kit and method for rapidly detecting SLCO1B1 and ApoE gene polymorphisms at the same time, to solve the problem of detecting SLCO1B1*1a(388A-521T), *1b(388G-521T),* 5(388A-521C), *15(388G-521C) and ApoE e2(388T-26T), e3(388T-526C), e4(388C-526C) gene polymorphisms require high sample processing and detection Shortcomings such as long cycle, complicated operation, high cost and low specificity

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  • Kit and method for simultaneously and rapidly detecting SLCO1B1 and ApoE gene polymorphism
  • Kit and method for simultaneously and rapidly detecting SLCO1B1 and ApoE gene polymorphism

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Embodiment Construction

[0092] In order to make the technical solutions of the present invention clearer and clearer to those skilled in the art, the present invention will be further described in detail below in conjunction with the examples and accompanying drawings, but the embodiments of the present invention are not limited thereto.

[0093] In this embodiment, the genotype is interpreted according to the graphic results. The kit for rapidly detecting SLCO1B1 and ApoE gene polymorphisms provided in this embodiment includes SLCO1B1*1a(388A-521T), *1b( Reagents The box includes sample processing reagents for rapid processing of human whole blood samples, premixed single-serving gene detection reagents, positive control substances and negative control substances, including SLCO1B1 gene detection reagents and ApoE gene detection reagents respectively.

[0094] In this embodiment, this embodiment detects SLCO1B1*1a(388A-521T), *1b(388G-521T), *5(388A-521C), *15(388G-521C), and ApoE e2 (388T-26T), e3...

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Abstract

The invention discloses a kit and a method for simultaneously and rapidly detecting SLCO1B1 and ApoE gene polymorphism, and belongs to the technical field of gene detection. The kit comprises a sampletreating reagent, a gene testing reagent, a positive reference substance and a negative reference substance, the sample treating reagent is used for rapidly treating a human whole blood sample, and the gene testing reagent is separately packaged in a premixed single portion manner and includes an SLCO1B1 gene testing reagent and an ApoE gene testing reagent. According to the kit, convenient, rapid and efficient detection is achieved based on guarantee of detection specificity and sensitivity, detection steps are decreased, production cost and detection cost are reduced when detection reactiontime is shortened, and clinical detection, analysis and application are facilitated.

Description

technical field [0001] The invention relates to a gene polymorphism detection kit and method, in particular to a kit and method for simultaneously rapidly detecting SLCO1B1 and ApoE gene polymorphisms, belonging to the technical field of gene detection. Background technique [0002] The SLCO1B1 gene is located on human chromosome 12 and encodes an organic anion transporting polypeptide (OATP1B1). One haplotype: *1a(388A-521T), *1b(388G-521T), *5(388A-521C), *15(388G-521C), in the Chinese population, SNP 388A>G accounts for about 74% , 521T>C is about 14%. Among the four haplotypes, *1a has the highest incidence rate, accounting for about 61.5%, followed by *1b (28%) and *15 (10.5%). Not found. [0003] At present, statins are the most effective drugs for lowering low-density lipoprotein (LDL-C) levels and thereby reducing the risk of cardiovascular disease. However, different individuals respond differently to different statins, and the key factor leading to the diff...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/156C12Q2600/16C12Q2600/166
Inventor 何顺清王家亮翁晟璐
Owner 江苏美因康生物科技有限公司
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