Kit used for SNP detection of personalized medicine relaed genes of statin lipid-lowering medicine and detecting method thereof

Abstract

The invention discloses a kit used for SNP detection of personalized medicine related genes of statin lipid-lowering medicine and a detecting method thereof. The kit comprises two forward primers and a reverse primer for detecting a locus rs17238540 of an the HMGCR gene, two forward primers and a reverse primer for detecting a locus rs7412 of an the APOE gene, two forward primers and a reverse primer for detecting a locus rs429358 of an the APOE gene, and two forward primers and a reverse primer for detecting a locus rs4149056 of an the SLCO1B1 gene. The kit used for the SNP detection of the personalized medicine related genes of the statin lipid-lowering medicine can conduct high throughput detection on the four loci, and a result of genotyping can be visually distinguished through software so as to achieve the purposes of quantitatively controlling metering of the statin lipid-lowering medicine and minimizing side effects.

Description

technical field [0001] The invention relates to a kit for multiple gene detection and a detection method thereof, in particular to a kit and a detection method for SNP detection of genes related to individualized medication of statin lipid-lowering drugs. Background technique [0002] Statins are hydroxymethylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, which competitively inhibit endogenous cholesterol synthesis rate-limiting enzyme (HMG-CoA) reductase, Blocking the metabolic pathway of valonate in cells reduces the synthesis of cholesterol in cells, thereby stimulating the increase in the number and activity of low-density lipoprotein (LDL) receptors on the surface of cell membranes (mainly liver cells) and clearing serum cholesterol increase, decrease in level. While statins lower lipids, they also bring toxic side effects such as decreased liver function and muscle pain. [0003] The HMGCR gene encodes the rate-limiting enzyme of cholesterol synthesis and is an ...

AI Technical Summary

Problems solved by technology

Sequencing technology is recognized as the gold standard for detecting gene mutations, but it is difficult to form due to high equipment costs, long detection cycle, low detection throughput, low detection sensitivity, high technical requirements for experimenters, and cumbersome results determination steps. Commercial diagnostic products; the detection sensitivity of restriction fragment length polymorphism analysis is also not high, and the operation steps are cumbersome. The detection results still need to be verified again by the next generation sequencing method, especially when the sample size is large, it is easy to cause crossover of PCR products Contamination and prone to insufficient or excessive enzyme digestion and false negative or false positive results, so it cannot be applied clinically

Chip detection is not suitable for the development of clinical detection kits due to its poor accuracy and repeatability of detection results and long experimental cycle.

The detection method of fluorescent quantitative PCR has the advantages of low cost, high sensitivity, strong specificity, and good repeatability of results. It is a very good detection method for detecting SNPs, but the ordering cost of conventional Taqman probe method probes is too high , it is difficult to ensure that all primers and probes can achieve very good amplification results under the same amplification conditions when multiple sites are detected at the same time

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