Primer and probe composition for detecting SLCO1B1 and APOE (apolipoprotein E) gene polymorphism and kit and method

Abstract

The invention relates to a primer and probe composition for detecting SLCO1B1 and APOE (apolipoprotein E) gene polymorphism and a kit and a method. The primer and probe composition for detecting SLCO1B1 and APOE gene polymorphism comprises a first primer and a first probe and a second primer and a second probe, wherein the first primer and the first probe are used for detecting an SLCO1B1 locus; the second primer and the second probe are used for detecting an APOE locus; nucleotide sequences of the first primer are shown in SEQ ID No.1-4; nucleotide sequences of the first probe are shown in SEQ ID No.9-10; nucleotide sequences of the second primer are shown in SEQ ID No.5-8; and nucleotide sequences of the second probe are shown in SEQ ID No.11-12. The primer and probe composition has theadvantages of being high in detection flux and simple and convenient to operate.

Description

technical field [0001] The invention relates to gene detection technology, in particular to a primer and probe composition, kit and method for detecting SLCO1B1 and APOE gene polymorphisms. Background technique [0002] According to my country's epidemiological survey, the overall prevalence of dyslipidemia in Chinese adults was as high as 40.4% in 2012, which was a substantial increase compared with 10 years ago, and the increase in serum total cholesterol (TC) levels will lead to cardiovascular events in China in the future An increase of about 9.2 million. Dyslipidemia characterized by elevated low-density lipoprotein cholesterol (LDL-C) or TC is an important risk factor for atherosclerotic cardiovascular disease (ASCVD), so reducing LDL-C levels can significantly reduce the incidence of ASCVD and Danger of death. For every 1mmol / L (38mg / dL) reduction in LDL-C, the risk of major cardiovascular events can be reduced by 20%, and the risk of all-cause mortality can be reduc...

AI Technical Summary

Problems solved by technology

Main disadvantages: low sensitivity; special requirements for reagents and instruments, not easy to popularize; complicated operation, relatively high cost, slow speed and low throughput

[0016] The fluorescent PCR technology used in the above kits is the conventional fluorescent PCR method. One channel can only detect one mutation, and multiple genes need to be detected separately, which has problems such as low detection throughput and cumbersome operation; while the PCR-gene chip method, Although the detection throughput is high, there are problems such as long operation time, many steps and easy contamination

Neither of these two methods can meet the requirements of high clinical diagnostic throughput and simple and fast operation, and cannot be applied on a large scale.

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