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Primer-probe composition for detecting human SLCO1B1 and ApoE genetic typing, kit and detecting method

A technology for genotyping and detection reagents, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of poor specificity, low detection sensitivity, high price, etc., and achieve short experimental cycle, The effect of high sensitivity and easy operation

Inactive Publication Date: 2019-10-29
WUXI SHENRUI BIO PHARMA
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Problems solved by technology

Although the direct sequencing method has high sensitivity, it is expensive and cumbersome, and it is difficult to meet the clinical detection requirements; the chip hybridization method has low detection sensitivity and poor specificity, and is prone to false positive results; the high-resolution melting curve method has special requirements for equipment and is not suitable. Clinical promotion, and the detection sensitivity is not high

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  • Primer-probe composition for detecting human SLCO1B1 and ApoE genetic typing, kit and detecting method
  • Primer-probe composition for detecting human SLCO1B1 and ApoE genetic typing, kit and detecting method
  • Primer-probe composition for detecting human SLCO1B1 and ApoE genetic typing, kit and detecting method

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[0074] The present invention will be described in detail below in conjunction with the implementations shown in the drawings, but it should be noted that these implementations are not limitations of the present invention, and those of ordinary skill in the art based on the functions, methods, or structural changes made by these implementations Equivalent transformations or substitutions all fall within the protection scope of the present invention.

[0075] In this application, the term "sec" is a unit of time: second; the term "min" is a unit of time: minute; the term "rpm" is a unit of rotational speed: revolution per minute.

[0076] The human ApoE gene is located on the long arm of human chromosome 19, with a total length of about 3.7kb and 4 exons. The 4th exon shows a gene polymorphism, which makes the ApoE gene have three alleles of E2, E3, and E4 Among them, E2 (rs429358 is T, rs7412 is T), E3 (rs429358 is T, rs7412 is C), E4 (rs429358 is C, rs7412 is C) can be combine...

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Abstract

The invention provides a primer-probe composition for detecting human SLCO1B1 and ApoE genetic typing, a kit and a detecting method, the primer-probe composition comprises a specific primer, a specific probe and an internal reference agent for ApoE and SLCO1B1 genetic typing. According to the primer-probe composition, the kit and the detecting method, investigation of polymorphism of four types ofgenetic typing of SLCO1B1*1b(388G-521T), SLCO1B1*5(388A-521C), ApoE-E4(388C-526C) and ApoE-E2(388T-526T) in SLCO1B1 and ApoE genetic typing in a human body blood DNA sample is conducted synchronously, the advantages of high sensitivity, high specificity, low cost and high throughput, easy and convenient operation, and short experimental period are achieved, and the technical superiority of low cost of gene detecting is achieved.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer probe composition, a kit and a detection method for detecting human SLCO1B1 and ApoE genotyping. Background technique [0002] Statins are one of the most widely used lipid-lowering drugs in the world today. They have a significant effect on inhibiting the occurrence of cardiovascular and cerebrovascular diseases by effectively reducing blood lipids and stabilizing intravascular plaques. With the widespread use of statins, researchers have found that there are serious differences in the responses of different individuals to different statins, and the key factor leading to the differences is the different genetic characteristics of statins metabolism and transport in the liver, mainly by anion transport The polypeptide (OATP1BI) is encoded by the SLCOIBI gene and determined by the genetic polymorphism of apolipoprotein E (ApoE). [0003] Anion-transporting polypep...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q2531/113C12Q2545/101C12Q2563/107C12Q2545/113
Inventor 盛青松姚鲁帅
Owner WUXI SHENRUI BIO PHARMA
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