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Kit for detecting human hyperlipidemia sensitive genes

A technology of high blood lipids and kits, which is applied in the field of biomedicine, can solve the problems of high detection cost and low detection throughput, and achieve the effect of high throughput, low cost and rapid detection

Inactive Publication Date: 2021-02-26
为康(苏州)基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the drug metabolism gene detection technology for hyperlipidemia in the market is mostly carried out by fluorescent PCR and other technologies; the detection throughput is small and the detection cost is high

Method used

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  • Kit for detecting human hyperlipidemia sensitive genes
  • Kit for detecting human hyperlipidemia sensitive genes
  • Kit for detecting human hyperlipidemia sensitive genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] In this example, APOE, SLCO1B1-1, SLCO1B1-2, COQ2, and CYP3A5 are used as detection objects in human hyperlipidemia drug-sensitive gene mutation sites, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid Simultaneously detect five gene loci of APOE, SLCO1B1-1, SLCO1B1-2, COQ2, and CYP3A5.

[0062] The gene template of the experiment is: 30 wild-type blood samples and 5 mutant blood samples, which are tested at the same time, and finally the correct rate of negative and positive is used as the standard.

[0063] The first step, multiplex PCR reaction:

[0064] Primer set a mix (including primers APOEF1, APOER1, SLCO1B1F1, SLCO1B1R1, SLCO1B1F2, SLCO1B1R2, COQ2F1, COQ2R1, CYP3A5F1, CYP3A5R1) 1ul

[0065] PCR master Mix 1.2ul

[0066] H2O 0.8ul

[0067] DNA template 2ul

[0068] The reaction conditions of the multiplex PCR reaction are: 95° C. for 2 minutes, 95° C. for 30 seconds, 56° C. for 20 seconds, 72°...

Embodiment 2

[0077] In this example, APOE, SLCO1B1-1, SLCO1B1-2, COQ2, and CYP3A5 are used as detection objects in human hyperlipidemia drug-sensitive gene mutation sites, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid Simultaneously detect five gene loci of APOE, SLCO1B1-1, SLCO1B1-2, COQ2, and CYP3A5.

[0078] The gene template of the experiment is: 1 wild-type blood sample and 1 mutant blood sample, and 20 blood samples and 10 saliva samples are tested simultaneously.

[0079] The method for detecting human hyperlipidemia drug sensitive gene mutation loci by using the above-mentioned multiplex PCR comprises the following steps:

[0080] (1) Sample processing and template extraction quality control:

[0081] The scope of application of samples includes whole blood, saliva, oral test strips and other specimens; for the extraction process, please refer to the instructions of the relevant finished commercial kits. This ex...

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Abstract

The invention discloses a kit for detecting human hyperlipidemia sensitive genes. The kit comprises an APOE primer group, an SLCO1B1-1 primer group, an SLCO1B1-2 primer group, a COQ2 primer group anda CYP3A5 primer group. The human hyperlipidemia drug sensitive genes APOE, SLCO1B1, COQ2 and CYP3A5 are taken as detection objects. Through a combination of the specific primers and combination of a nucleic acid mass spectrometry detection technology, a rapid, simple and accurate detection of variation conditions of the related genes is realized.

Description

technical field [0001] The invention relates to a kit for detecting human hyperlipidemia sensitive genes, belonging to the technical field of biomedicine. Background technique [0002] Pharmacogenomics studies have shown that the metabolism of hyperlipidemia drugs (pravastatin, atorvastatin, rosuvastatin) is closely related to the mitochondrial gene MT-RNR1 in the human genome; , the side effects such as hearing loss or even deafness, nephrotoxicity and other side effects are significantly different from those of ordinary people; therefore, before using this type of drug, it is of great guiding significance to detect the mutation of related genes first. [0003] At present, the drug metabolism gene detection technology for hyperlipidemia in the market is mostly carried out by fluorescent PCR and other technologies; the detection throughput is small and the detection cost is high. [0004] The present invention aims to check the variation of human diabetes medication sensiti...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 田军龙张为
Owner 为康(苏州)基因科技有限公司
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