Hyperlipemia susceptible noninvasive detection kit (APOB and other genes)

Abstract

The invention provides a noninvasive detection kit for detecting hyperlipemia susceptible genes. The kit comprises specific primers and DNA (deoxyribonucleic acid) sequencing primers for detecting 3 mononucleotide polymorphism site genotypes (EcoRI on APOB gene, C677T (rs1801133) on MTHFR gene, and T521C (rs4149032) on SLCO1B1 gene), a PCR (polymerase chain reaction) component, a PCR product purification component, a DNA sequencing reaction component and the like. The kit evaluates the hyperlipemia attack risk level by detecting the 3 mononucleotide polymorphism site genotypes closely related to hyperlipemia on the gene level, and instructs people to prevent the occurrence of diseases in advance. The sampling method is an oral mucosa cell sampling method which is painless and noninvasive and avoids cross infection. The kit has the advantage of accurate and reliable sequencing detection result, and does not need to buy expensive imported special instruments; and thus, the method is easy to popularize.

Description

technical field [0001] This project belongs to the field of molecular biology and involves medicine and biotechnology. It specifically involves a non-invasive detection kit for hyperlipidemia susceptibility genes. Recommendations guide for hyperlipidemia. Background technique [0002] With the improvement of people's living standards, the number of patients with cardiovascular and cerebrovascular diseases is increasing year by year. Cardiovascular and cerebrovascular diseases have become another major killer of human death after cancer! A large number of studies have shown that: hyperlipidemia (hyperlipidemia, HLP), etc. Lipid metabolism disorder is an important cause of cardiovascular and cerebrovascular diseases that cannot be ignored. Hyperlipidemia is an important risk factor for coronary atherosclerotic heart disease (coronary heart disease), cerebral infarction, sudden cardiac death and other diseases, and is also an important risk factor for hypertension, impaired gl...

AI Technical Summary

Problems solved by technology

So far, more than 20 single nucleotide polymorphism (SNP) mutations have been found on the OATP1B1 gene, which will greatly reduce the ability of cells to uptake OATP1B1 substrates and be related to abnormal transport functions

Medical workers genotyped 24 healthy males from across the country and their absorption of pitavastatin, a commonly used drug for hyperlipidemia, found that the absorption of pitavastatin in the 52l T > C mutation group was significantly lower than that of the wild-type 521TT Homozygous, which indicates that mutations in this gene will reduce the normal absorption of the drug, thereby affecting the therapeutic effect of hyperlipidemia