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Primer combination for guiding related gene detection of individualized medications of risuvastatin drug, kit and method

A technology for rosuvastatin and gene detection, which is applied in the field of primer combinations and kits for guiding gene detection of rosuvastatin drug individualized medicine, can solve the problems of adverse reactions of clinical medication, no kit products, etc. The effect of medical cost, rapid detection, and simple interpretation of results

Inactive Publication Date: 2019-10-29
SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the technical problems of traditional clinical experience medical diagnosis and rosuvastatin drug administration, which are prone to clinical adverse reactions and there is no suitable kit product, the present invention provides a simple operation, rapid detection, high accuracy and specificity A good primer combination, kit and method for guiding the detection of genes related to individualized drug use of rosuvastatin

Method used

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  • Primer combination for guiding related gene detection of individualized medications of risuvastatin drug, kit and method
  • Primer combination for guiding related gene detection of individualized medications of risuvastatin drug, kit and method
  • Primer combination for guiding related gene detection of individualized medications of risuvastatin drug, kit and method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: the preparation of kit

[0071] 1. Design and synthesis of primers and probes

[0072] Specific primers and Taqman probes were designed for the ABCG2 (rs2231142) and SLCO1B1 (rs4149056) gene loci in the human genome (for the sequence, refer to the human genome sequence published in the NCBI database), using Primer Premier 5.0 and Primer Express 3.0 software.

[0073] Specific primers and probe sequences are shown in Table 1 below:

[0074] Table 1 Primer and probe sequence and length information

[0075]

[0076] 2. Selection of reference substance

[0077] The positive control substance one is respectively ABCG2 (rs2231142), SLCO1B1 (rs4149056) gene site wild-type plasmids;

[0078] The two positive control substances are plasmids heterozygous for ABCG2 (rs2231142) and SLCO1B1 (rs4149056) gene loci wild type and mutant type at a quantitative ratio of 1:1;

[0079] The three positive control substances are ABCG2 (rs2231142) and SLCO1B1 (rs4149056) g...

Embodiment 2

[0092] Example 2: A method for detecting genes related to individualized drug use of rosuvastatin using the above kit

[0093] 1. Biological samples

[0094] The biological materials used in the present invention are all from within the company. Anticoagulant for the remaining 500 cases tested in the company during September-December 2018.

[0095] 2. Take the DNA extracted from the sample to be tested as a PCR template:

[0096] The DNA extraction kit is the extraction kit "Blood Genomic DNA Extraction Kit" produced by Tiangen Biochemical Technology (Beijing) Co., Ltd. (Cat. No.: DP318).

[0097] 1) Take 400ul of whole blood, add 800ul of cell lysate CL, mix well, centrifuge at 10000rpm / 11500×g for 1min, discard the supernatant, and repeat once if the lysis is not complete.

[0098] 2) Add 200ul buffer GS to the precipitation, mix well, then add 20ul proteinase K, mix well.

[0099] 3) Add 200ul buffer solution GB, mix well, 56°C, 10min, invert several times during the pe...

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Abstract

The invention discloses a primer combination for guiding related gene detection of individualized medications of a risuvastatin drug. The primer combination is characterized by including specific primers and probes for an ABCG2(rs2231142) gene locus and an SLCO1B1(rs4149056) gene locus. The invention further discloses a kit containing the primer combination and an analytical method of the kit. According to the primer combination, the characteristics of easy operation, the economical efficiency, rapid detection, the high accuracy, the good specificity and easy result interpretation are achieved, the polymorphism of related genes of the individualized medications of the risuvastatin drug is rapidly and accurately measured to lower the adverse reaction of common medications of the rosuvastatin drug in clinic, the medical cost is lowered, and social resources are saved.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a primer combination, a kit and a method for guiding the detection of genes related to individualized drug administration of rosuvastatin. Background technique [0002] Hyperlipidemia is a common metabolic disease in clinical practice. It is a disease in which one or more lipids in plasma are higher than the normal index due to abnormal fat metabolism or turnover. Hyperlipidemia is a chronic disease with high blood lipid levels. The etiology of hyperlipidemia is complex, and age, sex, heredity, environment and bad living habits can all lead to the occurrence of the disease. Clinical studies have shown that hyperlipidemia is a predisposing factor for various cardiovascular diseases, such as atherosclerosis and coronary heart disease. In recent years, with the continuous improvement of people's material life, the incidence of this disease has also increased year by year...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 宣涛刘颖杜晴晴赵艳伟孙子奎
Owner SHANGHAI PASSION BIOTECHNOLOGY CO LTD
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