New method for detecting selenocysteine in living bodies

A technology of selenocysteine ​​and detection method, which is applied in the synthesis of polycarbonate micelles and the detection field of selenocysteine, to achieve the effect of easy storage and low cost

Active Publication Date: 2015-10-28
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are no public documents and patent applications on the synthesis of dinitrobenzenesulfonyl-modified biodegradable polycarbonate micelles and their detection of selenocysteine ​​in tissues.

Method used

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  • New method for detecting selenocysteine in living bodies
  • New method for detecting selenocysteine in living bodies

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Experimental program
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Embodiment Construction

[0015] Please refer to the accompanying drawings for the reaction route for synthesizing the dinitrobenzenesulfonyl-modified biodegradable polycarbonate micelle compound provided by the present invention.

[0016] Below in conjunction with concrete preparation example, the present invention will be further described:

[0017] Synthesis of PMPC-Dns micelles

[0018] Synthesis of 3-Azido-1-propanol

[0019] Into a 50mL reaction tube, add 1.02g 3-bromopropanol, 0.95g sodium azide, then add 10mL distilled water, react at 80°C for 18h, stop heating, cool to room temperature, add ethyl acetate for extraction, take the upper layer, Then washed with saline solution, dried over anhydrous magnesium sulfate, filtered, the filtrate was collected, and the solvent was removed to obtain colorless 3-azido-1-propanol with a yield of 76.5%.

[0020] Synthesis of propyl 2,4-dinitrobenzenesulfonate (Dns-N 3 )

[0021] Into a 50mL round bottom flask, add 0.57g 3-azidopropanol, 1.56mL triethyla...

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Abstract

The invention reasonably designs and prepares a polycarbonate polymer, namely, PMPC-Dns, modified through 2,4-dinitrobenzene sulfonyl so as to detect selenocysteine in living bodies. The compound can wrap fluorescent medicine doxorubicin (DOX) and selectively respond to Sec and a selenol compound without being interfered with by biological mercaptan, amine and alcohol. Formation of micelle, responses of Sec, cytotoxicity of probes and medicine release of the Sec responses are researched. The PMPC-Dns probes can be applied to imaging of endogenous Sec in cervical cancer tissue and hela cells under the physiological conditions, and the PMPC-Dns probes can conduct imaging and release medicine wrapped in the probes at the same time. The work opens up a road for understanding the effects of Sec in physiology and pathology systems and tumor xenograft model systems, and a method is provided for controlled releases of hydrophobicity molecules in target molecules in the biomedicine application.

Description

【Technical field】 [0001] The invention belongs to the field of organic synthesis and detection, and in particular relates to a synthesis method of dinitrobenzenesulfonyl-modified biodegradable polycarbonate micelles and the detection of selenocysteine ​​in tissues. Amphiphilic polymers were facilely synthesized by ring-opening polymerization (ROP) of cyclic carbonates with polycarbonate as the hydrophobic chain. Coupling reaction of azide and alkyne by Huisgen 1,3-dipolar cycloaddition under the catalysis of copper leads to the formation of Sec-responsive polycarbonate micelles. 【Background technique】 [0002] In 1969, Se's anticancer ability was discovered in the United States, and selenium intake has been found to be associated with many diseases. Low molecular weight selenium compounds such as methylselenol and selenocysteine ​​(Sec) are key metabolites and have been found to be important in cancer prevention. In particular, selenocysteine, which is an important low-mol...

Claims

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Application Information

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IPC IPC(8): C08G64/42G01N21/64
Inventor 邱仁华南艳霞许新华赵文杰
Owner HUNAN UNIV
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