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Microfluid system used for detecting circulating tumor cells (CTCs) of esophageal squamous cell carcinoma, and application thereof

A microfluidic system and tumor cell technology, applied in the field of microfluidic systems based on silicon nanowire substrates, can solve the problems of insufficient purity and sensitivity of CTCs, CTCs can not be used continuously, etc., to achieve a good cell capture environment and ensure biological safety , the effect of improving the purity

Inactive Publication Date: 2017-08-22
无锡准因生物科技有限公司
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects that the purity and sensitivity of the current CTC detection are not high enough, and the captured CTCs cannot be used for subsequent research, and provide a silicon nanowire-based method for detecting circulating tumor cells in esophageal squamous cell carcinoma. Substrates for Microfluidic Systems

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  • Microfluid system used for detecting circulating tumor cells (CTCs) of esophageal squamous cell carcinoma, and application thereof
  • Microfluid system used for detecting circulating tumor cells (CTCs) of esophageal squamous cell carcinoma, and application thereof
  • Microfluid system used for detecting circulating tumor cells (CTCs) of esophageal squamous cell carcinoma, and application thereof

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Embodiment 1

[0043] A microfluidic system for detecting circulating tumor cells in esophageal squamous cell carcinoma, prepared by the following steps:

[0044] 1. Fabrication of PDMS microfluidic chip

[0045] The PDMS microfluidic pipeline system includes an inlet and an outlet. The PDMS microfluidic pipeline system is divided into 2 parallel microfluidic channels from the inlet, and each branch is provided with rounded corners. Each channel is 25mm long and 2mm wide; The width of the fishbone structure in the fluid channel is 50 microns, the pitch is 50 microns, and the height is 30 microns, such as figure 1 As shown, 1 is the fishbone structure, 2 is the inlet, 3 is the outlet, and 4 is the microfluidic channel. Figure 5 Shows the effect of the fillet design of the diversion on the flow rate. exist Figure 5 , the vertical axis and the horizontal axis are the size of the flow channel, the unit is mm, and the legend on the right shows the size of the flow velocity; from Figure 5 I...

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Abstract

The invention discloses a microfluid system used for detecting CTCs of the esophageal squamous cell carcinoma. The microfluid system is prepared through the following steps: 1, preparation of a microfluid pipeline system which is divided into two or more parallel microfluid channels from an inlet, wherein the microfluid channels are provided with a plurality of fishbone structures; 2, etching of a silicon nanowire substrate; 3, surface modification and biotin bio-functionalization of silicon nanowires; and 4, modification of the silicon nanowire substrate and assembling of a PDMS microfluid chip. In use of the system, cells of a to-be-detected sample and 200 [mu]L of a PBS solution of biotin-modified p-EpCAM and p-cKit are subjected to co-incubation for 1 h, and then a co-incubation product is injected into the microfluid system for detection. The microfluid system provided by the invention can recognize and bind to CTCs with extremely low content, and is applicable to traditional immunofluorescence dyeing; obtained detection results and phenotypic analysis results can be subjected to further specific release; so purification of CTCs is realized, and the purity of CTCs is improved so as to meet requirements of gene detection limits.

Description

technical field [0001] The invention relates to a microfluidic system for detecting circulating tumor cells, in particular to a microfluidic system based on a silicon nanowire substrate for detecting circulating tumor cells in esophageal squamous cell carcinoma. Background technique [0002] With the increase of pollution level, the incidence rate of tumor has been higher than 1 / 3. The diagnostic methods for tumors mainly include: pathology (biopsy section); imaging (ultrasound, X-ray, CT or PET, etc.) and serology (serum tumor-associated proteins, such as CA-125, CA-199 or CEA, etc.). However, these methods have their own defects and cannot provide help for changing tumors and diagnosis and early warning. Currently clinically recognized human peripheral blood circulating tumor cell (circulating tumor cell, referred to as CTC) detection has been recognized as one of the best detection means. [0003] In 2004, the US Drug and Food Administration approved the first technical...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/531
CPCG01N33/57407G01N33/531
Inventor 林泉俞珏华程禹
Owner 无锡准因生物科技有限公司
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