Kit integrating three proteins such as Kindlin-2, Myosin-9 and Annexin II for prognosis evaluation of patient suffering from esophageal squamous cell carcinoma

A technology of esophageal squamous cell carcinoma and a kit is applied in the field of kits for predicting the prognosis of patients, which can solve the problems of unclear prognosis of patients with Kindlin-2 expression.

Inactive Publication Date: 2015-03-11
SHANTOU UNIV MEDICAL COLLEGE
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Problems solved by technology

However, in esophageal squamous cell carcinoma, which ranks fourth in the mortality rate of malignant tumors in China, the expression of Kindlin-2 and its relationship with the prognosis of patients are still unclear

Method used

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  • Kit integrating three proteins such as Kindlin-2, Myosin-9 and Annexin II for prognosis evaluation of patient suffering from esophageal squamous cell carcinoma
  • Kit integrating three proteins such as Kindlin-2, Myosin-9 and Annexin II for prognosis evaluation of patient suffering from esophageal squamous cell carcinoma
  • Kit integrating three proteins such as Kindlin-2, Myosin-9 and Annexin II for prognosis evaluation of patient suffering from esophageal squamous cell carcinoma

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Embodiment Construction

[0013] We collected archival wax blocks of surgical specimens from 110 patients with esophageal squamous cell carcinoma from 2000 to 2006. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0014] The steps of immunohistochemical hypersensitivity two-step method are as follows:

[0015] After the specimens were serially sectioned, dewaxed with xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method.

[0016] The specific method is as follows:

[0017] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, incubate with Annexin II, Kindlin-2 and Myosin-9 antibodies overnight at 4°C;

[0018] (2) After the sections were incubated with Polymer enhancer and Polymer polymer, the color was developed by DAB;

[0019] (3) The sections were counterstaine...

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Abstract

The invention aims to provide a reagent capable of detecting the expression condition of a target protein in a paraffin-embedded tissue, and particularly provides a kit used for evaluating the prognosis information of a patient suffering from the esophageal squamous cell carcinoma. The kit comprises antibodies of the three target proteins, namely Annexin II, Kindlin-2 and Myosin-9, and further comprises goat serum, 0.01 M citrate repair liquid, 3% H2O2, a Polymer reinforcing agent, a Polymer, a DAB color reagent and a PBS solution. The kit can be used for evaluating the prognosis of the patient; compared with the international TNM staging, the kit is simpler and easier to use, and higher in both sensitivity and specificity, and is more suitable for the prognosis evaluation of Chinese suffering from the esophageal squamous cell carcinoma.

Description

technical field [0001] The invention relates to a kit for combining multiple protein antibodies and using an immunohistochemical hypersensitive two-step method to detect the expression of specimens from patients with esophageal squamous cell carcinoma, thereby predicting the prognosis of patients. Background technique [0002] Esophageal squamous cell carcinoma (ESCC), referred to as esophageal squamous cell carcinoma, is a common malignant tumor of the upper gastrointestinal tract in my country and ranks fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal squamous cell carcinoma each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal squamous cell carcinoma in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yant...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/52
Inventor 许丽艳曹卉慧许秀娥吴健谊李恩民
Owner SHANTOU UNIV MEDICAL COLLEGE
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