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Long-chain non-coding RNA LINC01419 molecular marker of esophageal squamous cell carcinoma and application thereof

A long-chain non-coding, esophageal squamous cell carcinoma technology, applied in DNA/RNA fragmentation, recombinant DNA technology, microbial assay/examination, etc., can solve the problems of early esophageal cancer patients losing treatment opportunities and unclear diagnosis, etc.

Inactive Publication Date: 2018-09-28
汕头大学医学院附属肿瘤医院
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Problems solved by technology

Due to the unclear diagnosis caused by the level of technology, it is likely that some patients with early esophageal cancer will lose the best treatment opportunities

Method used

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Embodiment

[0020] Tissue specimens from 38 freshly resected ESCC cases and 38 cases with normal morphology were collected and LINC01419 levels were quantified. Firstly, LINC01419 was silenced in vitro to detect its effect on the pathogenesis of ESCC; then the link between LINC01419 and GSTP1 gene promoter was verified by dual luciferase reporter gene assay; ESCC was then treated by gene demethylation reagent 5-Aza-CdR Cells, observe the growth of ESCC cells and their sensitivity to 5-FU; use CCK-8 detection to determine the relationship between LINC01419 and GSTP1 gene methylation; finally conduct a tumorigenicity test in nude mice to verify the effect of LINC01419 on ESCC The effect occurred and the sensitivity of ESCC cells to 5-FU was assessed in vivo. In summary, by overexpressing LINC01419, silencing LINC01419, and adding the DNA methyltransferase inhibitor 5-Aza-CdR, ESCC cells were evaluated for their proliferation and apoptosis abilities and their ability to recruit DNA methyltra...

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Abstract

The invention discloses a long-chain non-coding RNA marker for assisting diagnosis of esophageal squamous cell carcinoma (ESCC). The long-chain non-coding RNA is LINC01419, the expression amount of the long-chain non-coding RNA is detected and can be used for auxiliary diagnosis or prediction of the esophageal squamous cell carcinoma, and the methylation status of a GSTP1 gene is monitored to predict the sensitivity of the esophageal squamous cell carcinoma to 5-FU chemotherapy. The expression level of a LINC01419 gene can be detected by an RT-PCR, real-time quantitative PCR, immunoassay, in situ hybridization, a chip or a high-throughput sequencing platform. The invention further discloses application of a methyltransferase inhibitor 5-Aza-CdR to regulate the expression of the long-chainnon-coding RNA LINC01419 and response to the 5-FU, and application of an inhibitor medicine including the long-chain non-coding RNA LINC01419 in treatment of the esophageal squamous cell carcinoma. The molecular marker of the esophageal squamous cell carcinoma is provided, a theoretical basis for mechanism research and clinical individualized treatment of the esophageal squamous cell carcinoma isprovided, and by monitoring the overexpression of the LINC01419 in the ESCC, a new treatment strategy for the clinical treatment of the ESCC can be provided.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a long-chain non-coding RNA marker for auxiliary diagnosis of esophageal squamous cell carcinoma and prediction of sensitivity of esophageal squamous cell carcinoma to 5-FU chemotherapy. Background technique [0002] Esophageal cancer is one of the ten most common malignant tumors in humans. Globally, there are more than 480,000 new cases of esophageal cancer each year, and more than 400,000 deaths. According to data reported by the American Cancer Society (ACS) in 2014, cancer mortality has declined steadily over the past two decades, and the overall risk of dying from cancer throughout life has dropped by 20%, but esophageal cancer is still the most common disease among 40-59-year-olds in the United States. The fifth most deadly tumor in men. China is an area with a high incidence of esophageal cancer in the world, especially in men, and the main tissue type is squamous cell carcin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154C12Q2600/158C12Q2600/178
Inventor 张述耀江艺江红佘玉奇陈晨陈建良庄珊珊陈云陈婉英
Owner 汕头大学医学院附属肿瘤医院
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