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Application of esophageal squamous cell carcinoma primary tumor line CH-H-2

A technology of CH-H-2 and esophageal squamous cell carcinoma, which is applied in the application field of CH-H-2, a primary tumor strain of esophageal squamous cell carcinoma, to achieve the effects of high metastatic potential and stable traits

Inactive Publication Date: 2012-11-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a kind of application of primary tumor strain CH-H-2 of esophageal squamous cell carcinoma, and this primary tumor strain CH-H-2 can be passaged through NOD / SCID mice for many times. Metastasis, an unavoidable bottleneck problem in the current treatment of esophageal squamous cell carcinoma, is of great significance and is an ideal object for basic research and preclinical application of esophageal squamous cell carcinoma

Method used

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  • Application of esophageal squamous cell carcinoma primary tumor line CH-H-2
  • Application of esophageal squamous cell carcinoma primary tumor line CH-H-2
  • Application of esophageal squamous cell carcinoma primary tumor line CH-H-2

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Embodiment 1

[0026] 1. Establishment of primary esophageal squamous cell carcinoma xenograft tumor lines

[0027] (1) Experimental animals: NOD / SCID mice, 4-6 weeks old, male or female, were purchased from the Experimental Animal Center of Second Military Medical University.

[0028] (2) Tumor tissue: Fresh esophageal tumor tissue specimens were obtained from a 48-year-old male patient with stage IIIa esophageal squamous cell carcinoma who underwent surgery in the Department of Thoracic and Cardiovascular Surgery, Changhai Hospital Affiliated to Second Military Medical University in October 2006.

[0029] (3) Tumor formation and passage expansion of primary tumor xenograft:

[0030] ①NOD / SCID mice were pretreated with cobalt-60-γ-ray irradiation 1 day before inoculation, and the irradiation dose was 2.5Gy / mouse.

[0031] ②The mice were anesthetized by intraperitoneal injection of pentobarbital sodium (0.05mg / g body weight), and the primary esophageal squamous cell carcinoma tissue was cut i...

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Abstract

The invention relates to an esophageal squamous cell carcinoma primary tumor line CH-H-2 which is used in mammals to produce esophageal squamous cell carcinoma cells. The primary tumor line CH-H-2 can be passed by NOD / SCID mice for multiple times, is significant in solving the inevitable bottleneck problem of distant metastasis in the current esophageal squamous cell carcinoma treatment, and is an ideal object for the basic research and clinical early application of esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the field of primary tumor xenograft tumor models, in particular to the application of a primary tumor strain CH-H-2 of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer (mainly squamous cell carcinoma) is the ninth most malignant disease in the world and is prevalent in many parts of the world, especially in developing countries. In China, the incidence of esophageal cancer is higher, ranking fourth among all tumors. The annual incidence and mortality of male esophageal cancer are 22.47 / 100,000 and 16.65 / 100,000, respectively, and the number of cases and deaths are 147,629 and 100,000 respectively. 109,435 people, the annual incidence and mortality of female esophageal cancer are 11.10 / 100,000 and 8.50 / 100,000, respectively, and the incidence and death are 68,853 and 52,711, respectively. The total annual incidence and death of esophageal cancer in my country is 216,482 and 162,146. The annu...

Claims

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Application Information

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IPC IPC(8): C12N5/09
Inventor 李白翎黄盛东袁扬龚德军陶婧倪逸倩
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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