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Application of long-chain non-coding RNA-ROR

A technology of long-chain non-coding and positive-sense strands, applied in the field of application of linc-ROR to prepare target markers in esophageal squamous cell carcinoma, and long-chain non-coding RNA-ROR, which can solve the problem that the mechanism of action is not fully elucidated And other issues

Inactive Publication Date: 2018-03-06
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, many studies have shown that there is abnormal expression of linc-ROR in various cancers, such as endometrial cancer, breast cancer, hepatocellular carcinoma, pancreatic cancer, etc., but its specific mechanism of action has not been fully elucidated

Method used

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  • Application of long-chain non-coding RNA-ROR
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  • Application of long-chain non-coding RNA-ROR

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1, linc-ROR can be used as a new target for auxiliary diagnosis and targeted therapy of esophageal squamous cell carcinoma

[0039] The sequence of linc-ROR is shown in SEQ ID No.1.

[0040] 1. Materials and methods

[0041] (1) Main reagents and consumables

[0042] 1. Main reagents and consumables for cell culture and transfection

[0043] EC9706 cell line (Shanghai Fuxiang Biotechnology Co., Ltd.); Eca-109 cell line (Shanghai Fuxiang Biotechnology Co., Ltd.), the product links are http: / / www.xiangbio.com / enstyle / product_9034853.htmlhttp: / / www.xiangbio.com / enstyle / product_8389053.html; culture medium RPMI 1640 (Gibco, USA); Opti-MEM I (Gibco, USA); DMSO (Beijing Solarbio); trypsin EDTA digestion solution ( Beijing Solarbio Company); penicillin-streptomycin mixed solution (Beijing Solarbio Company); 20×PBSSolution (Shanghai Sangon Bioengineering Company); fetal bovine serum (Israel Biological Industries Company); T25 cell culture flask (U.S. Corning Company...

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Abstract

The invention discloses application of long-chain non-coding RNA-ROR, in particular to application of a substance used for detecting linc-ROR and a substance used for inhibiting expression and / or functions of linc-ROR in preparation of products used for assisting diagnosis and treating esophageal squamous cell carcinoma respectively. Experiments show that inhibition of linc-ROR expression can inhibit proliferation, invasion, migration and drug resistance of esophageal squamous carcinoma cells and tumor stem cell-like characteristics and plays an important role in occurrence and development ofesophageal squamous carcinoma. Meanwhile, multiple microRNAs include has-miR-15b-5p, has-miR-33a-5p, has-miR-129-5p, has-miR-145-5p and has-miR-206 and can be targeted to linc-ROR. The invention provides a new target used for diagnosing and treating the esophageal squamous carcinoma, and a linc-ROR gene can be taken as a new target marker and used for preparing a new product used for diagnosing and / or treating the esophageal squamous carcinoma. The application disclosed by the invention has important clinical significance in improvement of diagnosis and treatment level of the esophageal squamous carcinoma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of a long-chain non-coding RNA-ROR (linc-ROR), in particular to the use of linc-ROR in the preparation of target markers in esophageal squamous cell carcinoma (referred to as esophageal squamous cell carcinoma) use in . Background technique [0002] Esophageal cancer is one of the most common digestive tract malignancies in the world, and squamous cell carcinoma (squamous cell carcinoma for short) is one of its main pathological types. China is an area with a high incidence of esophageal cancer. More than 50% of the morbidity and death of esophageal cancer in the world occur in China, and about 90% of them are esophageal squamous cell carcinoma. In recent years, the morbidity and mortality of esophageal cancer have been increasing continuously, which is an important problem that threatens human health. The five-year survival rate after operation is only 15%-30%. Therefo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61P35/00C12Q1/6886
CPCA61K31/713C12N15/113C12N2310/141C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 侯隽郁晓丹王良海张之钰李锋
Owner SHIHEZI UNIVERSITY
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