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Esophageal squamous cell carcinoma autoantibody molecular marker model and application thereof

A technology of molecular markers and autoantibodies, which is applied in the fields of analysis materials, biomaterial analysis, and measurement devices, can solve the problems of esophageal squamous cell carcinoma patient evaluation and survival rate of less than 15%, achieve good discrimination effect, simple detection method, Good sensitivity and specificity effect

Active Publication Date: 2019-02-15
汕头市颂美恩生物科技有限公司
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Problems solved by technology

At present, the overall 5-year survival rate of esophageal cancer is less than 15%. The severe situation is very worrying
At present, the tumor serum molecular markers detected clinically, such as carcinoembryonic antigen and squamous cell carcinoma antigen, are not specific for esophageal squamous cell carcinoma, and it is difficult to accurately diagnose patients with esophageal squamous cell carcinoma using these tumor serum molecular markers Evaluate

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  • Esophageal squamous cell carcinoma autoantibody molecular marker model and application thereof
  • Esophageal squamous cell carcinoma autoantibody molecular marker model and application thereof
  • Esophageal squamous cell carcinoma autoantibody molecular marker model and application thereof

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[0025] In order to make the purpose, technical solution and advantages of the present invention clearer, in this example, serum samples from 125 healthy persons and 388 esophageal squamous cell carcinoma patients before treatment were collected from 2011 to 2014. The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0026] The steps of the ELISA indirect method are as follows:

[0027] (1) Coating: Dilute recombinant ALDOA protein, recombinant ENO1 protein, recombinant p53 protein and recombinant NY-ESO-1 protein with 0.05M pH9.6 carbonate coating buffer to 0.4 μg / ml and 0.2 μg / ml, respectively. ml, 0.1 μg / ml and 0.1 μg / ml. Add 0.1ml of diluted recombinant ALDOA protein, recombinant ENO1 protein, recombinant p53 protein and recombinant NY-ESO-1 protein to the reaction wells of the 96-well ELISA plate, overnight at 4°C. The next day, the solution in the well was discarded, and washed 3 times with PBST, 1 minute each t...

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Abstract

The invention relates to an esophageal squamous cell carcinoma autoantibody molecular marker model and an application thereof. The molecular model mainly comprises an ALDOA autoantibody, an ENO1 autoantibody, a p53 autoantibody, and an NY-ESO-1 autoantibody, and can be used for preparing a kit for distinguishing esophageal squamous carcinoma patients and healthy medical examiners. The kit for detecting esophageal squamous cell carcinoma patients mainly comprises recombinant ALDOAD protein, recombinant ENO1 protein, recombinant p53 protein, and recombinant NY-ESO-1 protein. According to the esophageal squamous cell carcinoma autoantibody molecular marker model and the application thereof, the ENO1 autoantibody is found to be elevated in serum level in the esophageal squamous carcinoma patients for the first time, and is jointly detected with the ALDOA autoantibody, the p53 autoantibody, and the NY-ESO-1 autoantibody for distinguishing the esophageal squamous cell carcinoma patients andthe healthy medical examiners, and has a better distinguishing effect than a single index detection; and in addition, the detection method adopted by the invention is an enzyme-linked immunosorbent assay indirect method, is simple and convenient to implement, has good sensitivity and specificity, and is a method which is mature and reliable and can be widely used in base layer hospitals.

Description

technical field [0001] The invention relates to the field of esophageal squamous cell carcinoma, in particular to combining multiple autoantibodies and using an indirect enzyme-linked immunosorbent assay method to detect the level of serum specimens of patients with esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is a common malignant tumor of the upper gastrointestinal tract. It ranks eighth in incidence and sixth in mortality among various cancers worldwide. Histopathological examination results showed that esophageal cancer mainly includes two histopathological types: esophageal squamous cell carcinoma and esophageal adenocarcinoma. More than 90% of esophageal cancer cases in my country are esophageal squamous cell carcinoma; esophageal adenocarcinoma mainly occurs in the United States and other western countries. At present, the overall 5-year survival rate of esophageal cancer is less than 15%. The serious situation is very worrying....

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57407G01N33/57484G01N2800/60
Inventor 许镒洧彭裕辉许丽艳李恩民
Owner 汕头市颂美恩生物科技有限公司
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