Esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof

Abstract

The invention relates to an esophageal squamous cell carcinoma microenvironmental cell marker molecular model and application thereof. The molecular model mainly comprises a-SMA+ (invasive frontier) fibroblasts, CD163+ (interstitial) macrophages, CD117+ (protogenic layer) mastocytes and CD117+ (invasion frontier) mastocytes. The molecular model can be used to predict the prognosis of patients withesophageal squamous carcinoma and group adjuvant treatment sensitivity groups. A kit mainly includes an anti-CD117 protein antibody, an anti-CD163 protein antibody and an anti-a-SMA protein antibody.According to the invention, the combination of three molecules combined with histopathological features predicts patients' prognosis, which has a higher predictive effect than single index detectionand clearly distinguishes patients with esophageal squamous carcinoma adjuvant treatment sensitivity; an immune tissue chemical hypersensitivity two-step method on which the molecular model is based is a mature and reliable method which can be widely used in primary hospitals; and compared with international TNM staging, the molecular model has a higher predictive effect, and is simpler, easier, more sensitive and more specific.

Description

technical field [0001] The invention involves combining multiple protein antibodies and histopathological features, and using the immunohistochemical hypersensitive two-step method to detect the expression of esophageal squamous cell carcinoma patient specimens, thereby predicting the prognosis of patients and the sensitivity of adjuvant therapy. Background technique [0002] Esophageal squamous cell carcinoma (ESCC), referred to as esophageal squamous cell carcinoma, is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal squamous cell carcinoma each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal squamous cell carcinoma in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in ...

AI Technical Summary

Problems solved by technology

However, there is currently no simple and effective method other than the international TNM staging that can more accurately predict the prognosis of patients with esophageal squamous cell carcinoma

However, TNM staging is cumbersome to predict the prognosis of patients. It is necessary to accurately evaluate the depth of tumor infiltration, the number of metastatic lymph nodes, and whether the tumor has distant metastasis. Moreover, the formulation of international TNM staging lacks a large number of case data of Chinese esophageal squamous cell carcinoma. Therefore, the applicability is relatively poor in evaluating the prognosis of patients in my country; there is a lack of available molecular markers in predicting the sensitivity of radiotherapy and chemotherapy

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