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Separating and purifying method of peanut bioactive peptide

A purification method and technology for active peptides, applied in the field of food protein processing, can solve the problems of high cost of chemical synthesis, high extraction cost, harmful to human body, etc., and achieve the effects of simple purification process, good functional activity and added value.

Inactive Publication Date: 2011-11-23
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method involves extracting certain types of pea nut proteins from their roots or stems by various methods such as solvent extraction techniques like liquid-liquid phase partitioning/extraction with organic solvents followed by filtration on different filters. These products have excellent properties that make them useful in applications related to prevention of damage caused by oxidiative stressors during periods of increased blood pressure levels associated with cardiovascular disease.

Problems solved by technology

This patents describes various techniques that help identify specific types of polypepittic peptiases called npepsins, specifically those found naturally occurring in plants like Pea nut shellfish. These peptisms contain multiple different parts - they may include certain essential ones – making them challenging to isolate and purity. Current processes involve expensive physical steps involving filtrations and redoxycycling/acidiuxyl transferring chemistry. Chemotherapy involves administering drugs directly into patients' bloodstream without affecting important organs involved. Physicochemical approaches require complicated procedures and often result in contamination issues.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take the peanut protein powder suspension with a mass percent content of 10%, adjust the pH value to 5.0, add 10FBG / g peanut protein powder Viscozyme L (compound plant hydrolytic enzyme, FBG is the enzyme activity unit of Viscozyme L), and extract in microwave / g In the synthesizer, microwave enzymolysis with 800w microwave power and 45°C for 5 minutes, then inactivate the enzyme in a 90°C water bath for 20 minutes, adjust the pH value to 8.0, add bromelain, trypsin, and ficin at a ratio of 2:2:3 The mixed compound enzyme, the addition amount is 5000U / g substrate (U is protease enzyme activity unit) in microwave extraction / synthesizer, with 800w microwave power and 45 ℃ conditions, microwave enzymolysis 6min, then, extinguish in 100 ℃ water bath Enzyme for 20 minutes, centrifuge at 5000r / min for 15 minutes at 20°C, and the supernatant is peanut protein powder protease enzymatic hydrolysis solution; peanut protein powder protease enzymatic hydrolysis solution is subjected ...

Embodiment 2

[0023] Take the peanut protein powder suspension with a mass percent content of 12%, adjust the pH value to 5.0, add 10FBG / g peanut protein powder Viscozyme L (composite plant hydrolytic enzyme, FBG is the enzyme activity unit of Viscozyme L), and extract in microwave / g In the synthesizer, microwave enzymolysis with 800w microwave power and 45°C for 5 minutes, then inactivate the enzyme in a 90°C water bath for 20 minutes, adjust the pH value to 8.0, add bromelain, trypsin, and ficin at a ratio of 2:2:3 The mixed compound enzyme, the addition amount is 6500U / g substrate (U is protease enzyme activity unit) in the microwave extraction / synthesizer, with 900w microwave power and 55 ℃ conditions, microwave enzymolysis 5min, then, extinguish in 100 ℃ water bath Enzyme for 20 minutes, centrifuge at 5000r / min for 15 minutes at 20°C, and the supernatant is peanut protein powder protease hydrolyzate; peanut protein powder protease hydrolyzate is subjected to an operating pressure of 0.4...

Embodiment 3

[0025]Take the peanut protein powder suspension with a mass percent content of 14%, adjust the pH value to 5.0, add 10FBG / g peanut protein powder Viscozyme L (compound plant hydrolytic enzyme, FBG is the enzyme activity unit of Viscozyme L), and extract in microwave / g In the synthesizer, microwave enzymolysis with 800w microwave power and 45°C for 5 minutes, then inactivate the enzyme in a 90°C water bath for 20 minutes, adjust the pH value to 8.0, add bromelain, trypsin, and ficin at a ratio of 2:2:3 Mixed complex enzymes, added in an amount of 7500U / g substrate (U is protease enzyme activity unit) in microwave extraction / synthesizer, with 1000w microwave power and 52°C conditions, microwave enzymolysis for 6.5min, then, in 100°C water bath Inactivate the enzyme for 20 minutes, centrifuge at 5000r / min for 15 minutes at 20°C, and the supernatant is the enzymatic hydrolyzate of peanut protein powder protease; It is a tubular nanofiltration membrane of 1000Da (Dalton), and the n...

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Abstract

The invention discloses a method for separating and purifying peanut bioactive peptide. The method comprises the steps that: peanut protein power protease enzymatic hydrolysate is prepared; the enzymatic hydrolysate is nanofiltrated; the filtrate is pre-condensed, such that a pre-condensed peanut protein power protease enzymatic hydrolysate is obtained; the pre-condensed enzymatic hydrolysate is desalted by a resin column; condensed solution is collected, such that desalted peanut protein power protease enzymatic hydrolysate is obtained; the desalted enzymatic hydrolysate is ultrafiltrated, separated, and condensed; the condensed solution is collected and lyophilized, such that peanut bioactive peptide is obtained. Peanut bioactive peptide provided by the invention has high purity, and has good functional activities of oxidative stability, bacteriostasis and ACE inhibitive activity. The peanut bioactive peptide can be applied in foodstuff industries as a natural foodstuff additive, and can be applied in auxiliary treatment of hypertension as a natural functional healthcare food material. The method has advantages of simple technology and easy operation, and is suitable for industrialized productions.

Description

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Claims

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Application Information

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Owner SHANDONG PEANUT RES INST
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