Walnut peptide having ACE inhibitory activity and preparation method thereof
A technology for inhibiting activity and walnut peptides, applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve the problems of low product activity, obstacles to industrialized production of products, and restrictions on the in-depth development and application of active peptides, and achieve low prices. , rich in raw materials, to meet the effect of large-scale industrial production
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specific Embodiment approach 1
[0037] Specific implementation mode one: as figure 1 As shown, the preparation method of the walnut polypeptide with ACE inhibitory activity in this embodiment is:
[0038] 1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil pressing and grind them, and pass through a 30-mesh sieve to obtain a pulverized walnut dregs sample.
[0039] 2. Preparation of crude walnut protein: Take crushed walnut dregs and mix them with 3 times the weight of water, soak for 10 minutes and make pulp, then adjust the pH to 7.5 with 1mol / L NaOH, stir in a water bath at 45°C for 0.5h, 3000rpm Centrifuge for 10min, remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 4000rpm for 20min, wash with an appropriate amount of deionized water to neutrality (pH7.0), and obtain the walnut crude protein. Washed twice with 95% ethanol and freeze-dried for later use.
[0040] 3. Preparation of activ...
specific Embodiment approach 2
[0044] Specific implementation mode two: as figure 1 Shown, the preparation method of the walnut polypeptide with ACE inhibitory activity of the present invention is:
[0045] 1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil extraction and pulverize them, and pass through a 50-mesh sieve to obtain a pulverized walnut dregs sample.
[0046] 2. Preparation of crude walnut protein: Take crushed walnut residue and mix it with 4 times of water, soak it for 20 minutes and make pulp, then adjust its pH to 8.5 with 1mol / L NaOH, stir in a water bath at 55°C for 1h, and centrifuge at 4000rpm for 10min , remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 5000rpm for 20min, wash to neutral (pH7.0) with an appropriate amount of deionized water, obtain walnut crude protein, use 95% washed twice with ethanol and freeze-dried for later use.
[0047] 3. Preparation of activ...
specific Embodiment approach 3
[0051] Specific implementation mode three: as figure 1 Shown, the preparation method of the walnut polypeptide with ACE inhibitory activity of the present invention is:
[0052]1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil pressing and grind them, pass through a 100-mesh sieve to obtain a pulverized walnut dregs sample.
[0053] 2. Preparation of crude walnut protein: Take crushed walnut dregs and mix them with 5 times of water, soak for 30 minutes and make pulp, then adjust the pH to 9.0 with 1mol / L NaOH, stir in a water bath at 65°C for 2 hours, and centrifuge at 6000rpm for 10 minutes , remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 8000rpm for 20min, wash to neutrality (pH7.0) with an appropriate amount of deionized water, obtain walnut crude protein, use 95% washed twice with ethanol and freeze-dried for later use.
[0054] 3. Preparation of act...
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