Walnut peptide having ACE inhibitory activity and preparation method thereof

A technology for inhibiting activity and walnut peptides, applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions, can solve the problems of low product activity, obstacles to industrialized production of products, and restrictions on the in-depth development and application of active peptides, and achieve low prices. , rich in raw materials, to meet the effect of large-scale industrial production

Active Publication Date: 2015-12-02
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are invention patents related to the technology of enzymatically hydrolyzing walnut protein to prepare walnut blood pressure-lowering peptide, but they all show certain deficiencies from different aspects: (1) the amino acid sequence identification after purification of the walnut active peptide prepared by these technologies is very important for research. Those who understand the biological mechanism of products have certain limitations; (2) Some pro

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  • Walnut peptide having ACE inhibitory activity and preparation method thereof
  • Walnut peptide having ACE inhibitory activity and preparation method thereof
  • Walnut peptide having ACE inhibitory activity and preparation method thereof

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specific Embodiment approach 1

[0037] Specific implementation mode one: as figure 1 As shown, the preparation method of the walnut polypeptide with ACE inhibitory activity in this embodiment is:

[0038] 1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil pressing and grind them, and pass through a 30-mesh sieve to obtain a pulverized walnut dregs sample.

[0039] 2. Preparation of crude walnut protein: Take crushed walnut dregs and mix them with 3 times the weight of water, soak for 10 minutes and make pulp, then adjust the pH to 7.5 with 1mol / L NaOH, stir in a water bath at 45°C for 0.5h, 3000rpm Centrifuge for 10min, remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 4000rpm for 20min, wash with an appropriate amount of deionized water to neutrality (pH7.0), and obtain the walnut crude protein. Washed twice with 95% ethanol and freeze-dried for later use.

[0040] 3. Preparation of activ...

specific Embodiment approach 2

[0044] Specific implementation mode two: as figure 1 Shown, the preparation method of the walnut polypeptide with ACE inhibitory activity of the present invention is:

[0045] 1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil extraction and pulverize them, and pass through a 50-mesh sieve to obtain a pulverized walnut dregs sample.

[0046] 2. Preparation of crude walnut protein: Take crushed walnut residue and mix it with 4 times of water, soak it for 20 minutes and make pulp, then adjust its pH to 8.5 with 1mol / L NaOH, stir in a water bath at 55°C for 1h, and centrifuge at 4000rpm for 10min , remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 5000rpm for 20min, wash to neutral (pH7.0) with an appropriate amount of deionized water, obtain walnut crude protein, use 95% washed twice with ethanol and freeze-dried for later use.

[0047] 3. Preparation of activ...

specific Embodiment approach 3

[0051] Specific implementation mode three: as figure 1 Shown, the preparation method of the walnut polypeptide with ACE inhibitory activity of the present invention is:

[0052]1. Pretreatment of walnut dregs: take the walnut dregs (also known as walnut dregs) after oil pressing and grind them, pass through a 100-mesh sieve to obtain a pulverized walnut dregs sample.

[0053] 2. Preparation of crude walnut protein: Take crushed walnut dregs and mix them with 5 times of water, soak for 30 minutes and make pulp, then adjust the pH to 9.0 with 1mol / L NaOH, stir in a water bath at 65°C for 2 hours, and centrifuge at 6000rpm for 10 minutes , remove the oil layer and extract the supernatant, adjust the pH of the supernatant to 4.5, leave it for 30min, centrifuge at 8000rpm for 20min, wash to neutrality (pH7.0) with an appropriate amount of deionized water, obtain walnut crude protein, use 95% washed twice with ethanol and freeze-dried for later use.

[0054] 3. Preparation of act...

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Abstract

The invention discloses walnut peptide having the ACE inhibitory activity and a preparation method thereof. The amino acid sequence of the walnut peptide is EPNGLLLPGY, and the preparation method of the walnut peptide comprises the steps of taking walnut pomace as raw materials, selecting appropriate protease, preparing walnut antihypertensive peptide coarse products according to the optimal enzymolysis technology, adopting the coupling chromatographic technique of the membrane technology for conducting fine preparation on the walnut antihypertensive peptide, adopting the high performance liquid chromatography for conducting further purification, and adopting the vacuum freeze drying or low-temperature spray drying technology for preparing final products. According to the walnut peptide, the walnut pomace is adopted as the raw materials for developing the protein peptide, the raw materials are abundant, the price is low, and the large-scale industrialized production can be satisfied; the walnut peptide has important significance in walnut protein product diversification and development and utlization of walnut biological active peptide in functional foodstuff, health food or medicine.

Description

technical field [0001] The invention belongs to the technical field of food-derived protein deep processing, and relates to a method for preparing walnut peptide with ACE inhibitory activity by using walnut residue as a raw material. Background technique [0002] Walnut, also known as walnut and Qiang peach, is listed as the four famous dry fruits in the world together with almond, cashew nut and hazelnut. The medicinal value of walnut is also very high, and it is widely used in traditional Chinese medicine. Chinese medicine believes that walnuts are warm in nature, sweet in taste, non-toxic, and have the effects of invigorating the stomach, nourishing blood, moistening the lungs, and nourishing the mind. Walnut is recognized as a high-quality vegetable protein resource, and the nutritional value of walnut protein is similar to that of animal protein. Therefore, in order to develop walnut resources well, it is extremely important to study the functional properties of walnu...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/18C07K1/16C07K1/14
Inventor 杜明刘猛王聪樊凤娇涂茂林石璞洁
Owner HARBIN INST OF TECH
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