Device and method for on-line monitoring of protein enzymolysis process based on in-situ real-time spectrum

Abstract

The invention discloses a device and a method for on-line monitoring of protein enzymolysis process based on in-situ real-time spectrum, and belongs to the field of on-line monitoring of protein enzymolysis process. The method comprises the following steps: conducting enzymolysis on gluten protein suspension liquids with different concentrations, conducting timing sampling in the enzymolysis process, and monitoring important parameters, such as the degree of hydrolysis, the hydrolysis concentration of an enzymolysis solution and the ACE inhibition ratio of the enzymolysis solution, in the enzymolysis process by a chemical method; quickly collecting the in-situ real-time spectrum of the collected enzymolysis solution; pretreating the collected spectrum; screening the optimal spectrum areas of the degree of hydrolysis, the hydrolysis concentration of the enzymolysis solution and the ACE inhibition ratio of the enzymolysis solution by adopting a synergy interval least square method; establishing a calibration model and a prediction model by adopting the synergy interval least square method; conducting in-situ real-time monitoring and reaction endpoint judging on the enzymolysis process by utilizing the prediction model. The enzymolysis process of the gluten protein suspension liquid with the substrate concentration of 10 g / L is monitored by utilizing the prediction model, and the goodness of fit of the predicted value and the measured value is relatively high.

Description

Technical field: [0001] The invention relates to the field of on-line monitoring of protein enzymatic hydrolysis process, in particular to a technology for on-line monitoring of gluten protein enzymatic hydrolysis process based on in-situ real-time near-infrared spectroscopy. Background technique: [0002] Wheat gluten is a by-product of starch processing, with a protein content of up to 85%, rich in hydrophobic amino acids, and is a very potential raw material for the preparation of blood pressure-lowering peptides by enzymatic hydrolysis. In the process of preparing antihypertensive peptides by enzymatic hydrolysis, the degree of hydrolysis of protein DH (Degree of hydrolysis), the concentration of polypeptides in the hydrolyzate, and the inhibition rate of ACE (The inhibition on angiotens in converting-Ienzyme inhibitory), which characterizes the antihypertensive activity of the hydrolyzate, are the three key indicators. An important basis for the end point of the enzymat...

AI Technical Summary

Problems solved by technology

However, these methods cannot be successfully applied to the reaction system of aqueous solution at present.

Images