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Fusarium graminearum caused rootstock rot seedling-stage resistance identification method

A technology for identifying the resistance of Fusarium graminearum, which is applied in the field of plant protection, can solve problems such as heavy workload, long identification time, and insufficient pathogenesis, and achieve the effects of reducing workload, shortening the test period, and achieving high coincidence

Active Publication Date: 2011-03-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
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Problems solved by technology

[0008] The object of the present invention is to: at present by Fusarium graminearum causes wheat root neck rot resistance identification to exist and be easily affected by environmental conditions, basically be to inoculate through seed, soil, seedling stage, there is insufficient morbidity, identification time A series of problems such as length and workload, etc., provide a method for identifying the resistance of wheat root neck rot caused by Fusarium graminearum at seedling stage

Method used

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  • Fusarium graminearum caused rootstock rot seedling-stage resistance identification method
  • Fusarium graminearum caused rootstock rot seedling-stage resistance identification method
  • Fusarium graminearum caused rootstock rot seedling-stage resistance identification method

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Embodiment 1

[0027] Identified varieties: 17 wheat varieties or strains imported from Australia are selected. Their field resistance to root neck rot caused by Fusarium graminearum is known. The data on disease resistance come from Queensland, Australia State 2007, 2008 and 2009 Wheat Variety Introductions (http: / / www.dpi.qld.gov.au / 265121.htm), and literature Mitter et al., PlantPathology, 2006, 55: 433-441; Li et al., Journal of Phytopathology, 2008, 156(11-12): 751-754.

[0028] Identification process:

[0029] The obtaining method of Fusarium graminearum suspension: Fusarium graminearum is F15, provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences [F15 of Fusarium graminearum has been published in literature (Ren Lijuan etc., wheat sheath blight resistance and Study on QTL mapping of head blight, Journal of Wheat Crops, 2007, 27(3): 416-420)]. The Fusarium graminearum strain F15 is activated and cultivated on a 1 / 4 strength PDA solid medium on a clean ben...

Embodiment 2

[0039] Using the identification steps in Example 1, 41 unknown wheat varieties or lines were selected. In order to facilitate the distinction of the 41 wheat varieties or lines, the identification numbers were sequentially defined as JS-1 to JS-41.

[0040] In order to test the repeatability of the method, 41 wheat cultivars or lines were designed for 2 independent identifications at different times, each identification included 3 repetitions, and each identification result was based on the average value of 3 repetitions. The average disease index and resistance evaluation of the 2 tests are listed in Table 2. The correlation analysis of the 2 test results shows that the correlation coefficient of the two disease indexes is 0.91, which shows that the repeatability of this seedling resistance identification method is relatively high. high.

[0041] Table 241 Wheat Varieties or Lines 2 Test Seedling Stage Resistance Inoculation Identification Results

[0042]

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Abstract

The invention relates to a fusarium graminearum caused rootstock rot seedling-stage resistance identification method comprising the following steps of sprouting measured wheat varieties or series seeds after sterilizing, and then inoculating activated fusarium graminearum; spreading the wheat seeds inoculated with the fusarium graminearum in a sterilized wet tissue, wrapping up, and keeping wet and culturing for 1 to 3 days under the shielding conditions, and then naturally growing for 11 to 13 days under natural lighting to form seedlings; immersing edges of the wet tissue in distilled water after the package layer of the surface of the wet tissue is dried during the natural growing period; and finally identifying the rootstock rot of the seedlings, and using the result as fusarium graminearum caused disease indexes of the measured wheat varieties or series seeds.

Description

technical field [0001] The invention relates to a method for identifying plant disease resistance, in particular to a method for identifying resistance to root neck rot of wheat caused by Fusarium graminearum at the seedling stage, and belongs to the technical field of plant protection. Background technique [0002] Multiple races of Fusarium can lead to the occurrence of wheat head blight (Fusarium Head Blight, FHB for short) and crown rot (CR for short), and are one of the main pathogens affecting wheat production. Wheat head blight caused by Fusarium graminearum, Fusarium yellow, etc. infecting the ears of wheat is an important disease of wheat in warm, rainy and humid climate areas, and is mainly prevalent in Canada, the United States, South America, Europe, and southern Africa and China, has become a worldwide wheat disease. my country is the country with the largest area of ​​wheat scab damage in the world. The winter wheat region in the middle and lower reaches of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00C12Q1/02
Inventor 杨学明马鸿翔姚金保周淼平
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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