Preparation method of rice bran protein peptide with ACE inhibitory activity

Abstract

The invention discloses a preparation method of rice bran protein peptide with ACE inhibitory activity. The method is characterized in that rice bran is adopted as a raw material, and comprises the following steps: firstly extracting rice bran protein, then preparing rice bran protein hydrolysate by adopting an enzyme hydrolysis method, and separating and purifying the rice bran ACE inhibitory peptide by adopting a hyperfiltration method, sephadex G-15 and a RP-HPLC method. According to the preparation method, the rich and cheap rice bran resource is effectively utilized to prepare the rice bran protein peptide with the high ACE inhibitory activity. The decompression function of the rice bran ACE inhibitory peptide can be maximally utilized, so that on one hand, the additional value of the rice bran is increased, and the good economic benefit and market value can be created; on the other hand, the morbidity of the hypertension can be effectively reduced, the high morbidity can be controlled, the human beings can be protected from the harm of the hypertension, the application value and social benefit are good, and the theoretical foundation and the experiment evidence can be provided for researching the structure and decompression mechanism of the rice bran protein ACE inhibitory peptide.

Description

technical field [0001] The invention belongs to the technical field of food and relates to a preparation method of rice bran protein peptide with ACE inhibitory activity. Background technique [0002] Rice is the largest grain variety in my country, with an annual output of about 180 to 200 million tons, accounting for 42% of the country's total grain output and about 35% of the world's total rice output. Rice bran is one of the by-products of rice. At present, my country has about 10 million tons of it. It is a renewable resource with a large amount and a wide range. However, the in-depth development and utilization of rice bran and the corresponding theoretical research are still at a low level in China, most of which are wasted as feed for livestock and poultry, and only 10% to 15% are used to extract oil or extract calcium phytate, inositol, oryzanol, etc. Higher value recycled products. [0003] Rice bran is a food, chemical and pharmaceutical raw material with great ...

AI Technical Summary

Problems solved by technology

Nevertheless, the current research on antihypertensive peptides such as ACE inhibitory peptides in my country is still in its infancy, and most of them focus on soybean peptides and corn peptides. [21] , there is only a very small amount of rice bran protein antihypertensive peptides reported; and there is no systematic method for the separation and purification of ACE inhibitory peptides, the primary structure identification, analysis of its relationship with ACE inhibitory activity and other related research are still blank; only in the experimental Laboratory basic research stage, and did not expand industrial production

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