Method for extracting fibroin peptide from mulberry silk

A technology of silk fibroin and silk, applied in fermentation and other directions, can solve the problems of difficult to achieve industrialized production, serious membrane pollution, etc., and achieve the effects of large processing capacity, simple process and low energy consumption

Inactive Publication Date: 2009-03-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the desalination process, membrane technologies such as ultrafiltration and electrodialysis are usually used for desalination, but the membrane pollution of this method is very serious, and it is difficult to realize industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=16%

[0011] 1.35kg waste silk is the Na of 5g / L through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. After the silk-dissolving solution is filtered through gauze to obtain a concentrated silk-dissolving solution, take 8L of the concentrated silk-dissolving solution, add 12L of water, adjust the pH to 8.5 with NaOH solution, and use protease (E / S=12AU / kg) for enzymatic hydrolysis at 60°C For the reaction, the degree of hydrolysis was controlled by the pH-stat method. After the reaction, the enzymolysis solution was heated to boiling, and the enzyme was inactivated for 10 minutes. After centrifugation and suction filtration, the filtrate was separated with a polysulfone ultrafiltration membrane with a molecular weight cut-off of 5000 Da. The ultrafilt...

Embodiment 2

[0013] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=20%

[0014] 1.7kg waste silk is 5g / L Na through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. After the silk-dissolving solution is filtered through gauze to obtain a concentrated silk-dissolving solution, take 10L of the concentrated silk-dissolving solution, add 15L of water, adjust the pH to 8.5 with NaOH solution, and use protease (E / S=24AU / kg) for enzymolysis at 60°C For the reaction, the degree of hydrolysis was controlled by the pH-stat method. After the reaction, the enzymolysis solution was heated to boiling, and the enzyme was inactivated for 10 minutes. After centrifugation and suction filtration, the filtrate was separated by a polysulfone ultrafiltration membrane with a molecular weight cut-off of 5000 Da. The ultrafiltration process par...

Embodiment 3

[0016] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=23%

[0017] 1.0kg waste silk is 5g / L Na through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. The silk-dissolving liquid was filtered through gauze to obtain a concentrated silk-dissolving liquid. Take 6L of the concentrated silk-dissolving liquid and add 10L of water. According to the same operating conditions as in Example 2, the blood pressure-lowering silk fibroin peptide powder was obtained. The recovery rate of silk fibroin peptide in the nanofiltration desalination test was 92.7%, and the ACE activity inhibition rate of the product was 56.8%.

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Abstract

The invention discloses a method for extracting silk peptide from cocoon fiber, which belongs to the technical field of preparation of the silk peptide. The method comprises the following steps: the cocoon fiber is added into a Na2CO3 solution for refining, filtrated and added into a CaCl2 solution for dissolution; protease for silk dissolution solution obtained after dissolution undergoes enzymatic hydrolysis reaction; after ultrafiltraiotn and nano-filtration separation of digest, a feed liquid is obtained; and the silk peptide powder is obtained after spray drying of the feed liquid. The method has the advantages that ACE inhibitory polypeptide is enriched through ultrafiltraiotn and nano-filtration separation of the digest; the efficiency of removing CaCl2 salt is high; the method has the advantages of large processing capacity, mild separation condition, simple flow, low energy consumption and so on; and the industrial production is easy to realize.

Description

technical field [0001] The invention relates to a method for extracting silk peptide from silk, and belongs to the technical field of preparation of silk peptide. Background technique [0002] Silk is a fibrous protein mainly composed of silk fibroin. The amino acid composition of silk fibroin is very unique, about 85% are glycine, alanine, serine and tyrosine. Silk peptide (silk peptide) is an intermediate product of silk fibroin hydrolysis. Silk peptide has the same amino acid composition as silk fibroin, but its molecular weight is much smaller than silk fibroin. Therefore, silk peptide has good water solubility , Excellent moisture absorption and moisturizing function, good acid and heat stability, viscosity changes slowly with concentration and so on. It is easily absorbed by the human body, and its digestibility is greatly improved compared with silk fibroin. It also has physiological functions such as lowering blood cholesterol levels, promoting alcohol metabolism, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 周凤娟薛照辉寇晓虹
Owner TIANJIN UNIV
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