Method for extracting fibroin peptide from mulberry silk
A technology of silk fibroin and silk, applied in fermentation and other directions, can solve the problems of difficult to achieve industrialized production, serious membrane pollution, etc., and achieve the effects of large processing capacity, simple process and low energy consumption
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Embodiment 1
[0010] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=16%
[0011] 1.35kg waste silk is the Na of 5g / L through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. After the silk-dissolving solution is filtered through gauze to obtain a concentrated silk-dissolving solution, take 8L of the concentrated silk-dissolving solution, add 12L of water, adjust the pH to 8.5 with NaOH solution, and use protease (E / S=12AU / kg) for enzymatic hydrolysis at 60°C For the reaction, the degree of hydrolysis was controlled by the pH-stat method. After the reaction, the enzymolysis solution was heated to boiling, and the enzyme was inactivated for 10 minutes. After centrifugation and suction filtration, the filtrate was separated with a polysulfone ultrafiltration membrane with a molecular weight cut-off of 5000 Da. The ultrafilt...
Embodiment 2
[0013] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=20%
[0014] 1.7kg waste silk is 5g / L Na through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. After the silk-dissolving solution is filtered through gauze to obtain a concentrated silk-dissolving solution, take 10L of the concentrated silk-dissolving solution, add 15L of water, adjust the pH to 8.5 with NaOH solution, and use protease (E / S=24AU / kg) for enzymolysis at 60°C For the reaction, the degree of hydrolysis was controlled by the pH-stat method. After the reaction, the enzymolysis solution was heated to boiling, and the enzyme was inactivated for 10 minutes. After centrifugation and suction filtration, the filtrate was separated by a polysulfone ultrafiltration membrane with a molecular weight cut-off of 5000 Da. The ultrafiltration process par...
Embodiment 3
[0016] Preparation of Hypotensive Silk Fibroin Peptide Powder with Degree of Hydrolysis (DH)=23%
[0017] 1.0kg waste silk is 5g / L Na through 50 times volume mass concentration 2 CO 3 After the solution is refined, the mass fraction placed in boiling is 40% CaCl 2 Dissolve in the solution for 5min and cool down rapidly. The silk-dissolving liquid was filtered through gauze to obtain a concentrated silk-dissolving liquid. Take 6L of the concentrated silk-dissolving liquid and add 10L of water. According to the same operating conditions as in Example 2, the blood pressure-lowering silk fibroin peptide powder was obtained. The recovery rate of silk fibroin peptide in the nanofiltration desalination test was 92.7%, and the ACE activity inhibition rate of the product was 56.8%.
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