6 results about "Holoenzymes" patented technology

This invention belongs to the field of biomedical technology, specifically involving the application of the MRPL41 gene / protein in the preparation of drugs for treating age-related sarcopenia. This invention is the first to demonstrate that MRPL41 is a key rate-limiting factor in the assembly of mitochondrial ribosomes in aging muscle satellite cells, establishing it as a core target for the treatment of age-related sarcopenia. Overexpression of MRPL41 can promote the assembly of mitochondrial 55S holoenzymes, improve the translation efficiency of mitochondrial-encoded proteins, repair mitochondrial dysfunction at its source, thereby promoting muscle repair, improving muscle atrophy, and achieving the treatment of age-related sarcopenia. This invention provides a precise microscopic target for the treatment of age-related sarcopenia, overcoming the limitations of existing clinical therapies (such as hormone replacement and simple nutritional supplementation) due to significant side effects and "anabolism resistance," providing a new direction for the development of innovative drugs specifically for the treatment of age-related sarcopenia, and has excellent application prospects.

The invention discloses a method for identifying a transcription factor binding DNA (deoxyribonucleic acid) sequence based on SpyCatch-CBM3-Tn5-His, which is characterized by comprising the following steps: (1) constructing a SpyCatch-CBM3-Tn5-His fusion protein, and the amino acid sequence of the SpyCatch-CBM3-Tn5-His fusion protein is as shown in SEQ ID NO: 9; (2) a KmRgt1-2 * SpyTag expression strain is constructed, and the nucleotide sequence of the KmRgt1-2 * SpyTag expression strain is shown as SEQ ID NO: 5; (3) incubating the SpyCatcher-CBM3-Tn5-His fusion protein and a linker sequence containing a mosaic sequence, so as to obtain an SCT complete enzyme; (4) immobilizing the SCT whole enzyme on the amorphous cellulose RAC, and incubating with a cell lysis solution containing KmRgt1-2 * SpyTag; and (5) cutting the DNA fragment combined with the protein by Tn5, and then constructing a sequencing library. The method does not need to depend on antigen-antibody reaction, avoids the influence of formaldehyde crosslinking on epitopes, is suitable for fungi, plants and other organisms with cell walls, has the characteristics of high resolution, low background and low cost, and can be applied to transcription factor regulation network research.

The present application relates to the field of food, especially to a rice protein and a preparation method thereof. The preparation method of the rice protein comprises the following steps: (1) grinding rice, adding a first amylase for enzymolysis, and separating to obtain a heavy phase to obtain a crude rice protein; (2) adding a glycerol triester lipase or a phospholipase to the crude rice protein obtained in step (1) for enzymolysis, and separating to obtain a heavy phase to obtain a defatted crude rice protein; (3) using a protease to perform enzymolysis on the defatted crude rice protein obtained in step (2) to obtain the rice protein, wherein the protease is one or two or more of a neutral protease, an alkaline protease and a papain. The present application successfully solves the problem of providing the protein content of the rice protein while improving the water holding capacity and oil holding capacity of the rice by adopting a whole enzymolysis combination technology, and improves the comprehensive performance of the rice protein.

The invention discloses a composite multienzyme-sodium alginate synergistic deodorization spray, a preparation method and application, the deodorization spray takes a composite multienzyme system as a core catalytic component, sodium alginate as a carrier and adsorption synergistic component, tea polyphenol as an antibacterial component, and a biological enzyme synergist as a performance strengthening component; through a quadruple synergistic mechanism of adsorption, catalysis, bacteriostasis and synergy, efficient removal of multiple types of peculiar smells such as sulfur, nitrogen, protein and fat in the household garbage and the pet excrement is achieved, meanwhile, breeding of peculiar smell source microorganisms is inhibited, and excellent ecological safety is achieved. The problems that an existing deodorant is single in enzyme system, incomplete in peculiar smell coverage, poor in enzyme stability, weak in continuous deodorization effect and the like are solved, and the deodorant is simple in preparation process, safe, free of residues, suitable for deodorization of families, pet places, garbage transfer stations and other scenes and wide in application prospect.

This invention discloses a method for constructing a dynamic enzyme constraint model based on a temporal neural network for unconventional yeast, aiming to solve the technical problems of staticity, rigid enzyme constraint updates, and poor numerical stability in traditional metabolic models. The method first constructs the framework of a static enzyme constraint model for unconventional yeast, integrates and completes enzyme kinetic parameters through deep learning, and establishes enzyme capacity constraints. It then constructs a dual-timescale coupling framework of macroscopic culture and microscopic metabolism, using enzyme constraint flux balance analysis to solve for instantaneous metabolic flux. An attention-enhanced long short-term memory network is designed to learn the temporal patterns of enzyme concentration, achieving dynamic allocation of enzyme resources. Physical laws are embedded into the loss function to train the network, and an adaptive time step ensures computational stability and efficiency. The dynamic metabolic trajectories of substrate, biomass, and products are output through iterative loops. This method is adaptable to various unconventional yeasts, can accurately simulate metabolic dynamics, and provides a general computational tool for the metabolic engineering modification of industrial strains.

This invention discloses a purine nucleoside phosphorylase mutant and its application in the preparation of nicotinamide ribomatate. The PNP mutant is a substitution of at least one amino acid residue from the wild-type PNPLf derived from *Leptodactylus fuscus*, specifically serine at position 244, threonine at position 247, and alanine at position 290, preferably the S244Y / T247W / A290F triple mutant. This invention also provides a one-pot enzymatic method for preparing NR malate, using inosine and nicotinamide as substrates. The PNP mutant catalyzes a phosphorylation-transglycosylation tandem reaction to generate NR, which is then reacted with malic acid in situ to form NR malate. The NR yield can reach over 92%, and the total yield of NR malate can reach over 82%. This method is green, efficient, highly selective, and simplified, avoiding the large-scale use of organic solvents, making it suitable for industrial production.