Triple Polymerase Chain Reaction(PCR)kit for identifying endogenous and exogenous avian leukosis viruses

A technology of avian leukosis virus and kit, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve the problems that cannot be ruled out and cannot be identified, and achieve high specificity and sensitivity

Inactive Publication Date: 2010-09-08
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the interference of endogenous ALV cannot be ruled out, and the detection of endogenous and exogenous ALV infection cannot be differentiated.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Triple Polymerase Chain Reaction(PCR)kit for identifying endogenous and exogenous avian leukosis viruses
  • Triple Polymerase Chain Reaction(PCR)kit for identifying endogenous and exogenous avian leukosis viruses
  • Triple Polymerase Chain Reaction(PCR)kit for identifying endogenous and exogenous avian leukosis viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0023] 1 Materials and methods

[0024] 1.1 Virus strains and main reagents Subgroup A ALV standard strain RAV-1 was purchased from China Veterinary Drug Administration, reticuloendotheliosis virus (Reticuloendotheliosis Virus, REV), chicken infectious bursal disease virus (Infectious bursal disease virus , IBDV) were purchased...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a triple Polymerase Chain Reaction(PCR)kit for simultaneously identifying endogenous and exogenous avian leukosis viruses, comprising DNA polymerase, d NTP, primers, a PCR buffer and double distilled water. The invention is characterized in that the primers consist of three pairs of primers, wherein the sequences of a first pair of the primers are shown as SEQ ID NO: 1 and SEQ ID NO: 2, the sequences of a second pair of the primers are shown as SEQ ID NO: 3 and SEQ ID NO: 4, and the sequences of a third pair of the primers are shown as SEQ ID NO: 5 and SEQ ID NO: 6. The invention has the advantages that by using the multiple PCR kit of the invention, whether the infection of the avian leukosis viruses occurs is simultaneously detected at the same reaction system and the endogenous and exogenous ALV can be identified, thereby saving time and reagents and providing an effective tool for rapid and early diagnosis on chicken flocks or chicken embryos; and the triple PCR kit has high specificity and sensitivity.

Description

technical field [0001] The invention relates to a kit for identifying avian leukosis virus, in particular to a triple PCR kit capable of simultaneously identifying endogenous and exogenous avian leukosis virus, which belongs to the field of detection of avian leukosis virus. Background technique [0002] Avian leukemia (Avian leukemia, AL) is a neoplastic disease in which certain cellular components in poultry hematopoietic tissue are overproliferated caused by the Avian Leukemia Virus (ALV) of the family Retroviridae (Zhao Zhenhua, Yang Yuying, Cheng Zi Qiang. Avian Leukemia [M]. Beijing: China Agricultural Press, 2006: 11-12.). Although the mortality rate of the disease is low, persistent infection of the disease will lead to immunosuppression and reduced productivity of the flock, thereby seriously damaging the economic value of the flock. [0003] According to the difference of pathogenicity and infection host, ALV can be divided into 10 subgroups A-J (Jin Yanling, Luo ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 韩凌霞司昌德周刚
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap