Screening and preserving method for Lolium L. embryogenic callus

A technology of embryogenic callus and callus, which is applied in the fields of obtaining ryegrass embryogenic callus and its regeneration ability maintenance, in vitro regeneration, and ryegrass callus induction, which can solve the problems such as difficulty in obtaining materials, and achieve Guaranteed differentiation rate, high-frequency differentiation regeneration efficiency, simple and easy operation

Active Publication Date: 2012-05-16
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mature seed embryos of ryegrass are very small, with a diameter of about 0.5-1.0mm; immature embryos are even

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening and preserving method for Lolium L. embryogenic callus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The following examples are used to further illustrate the present invention, but these specific experiments are exemplary, and they should not be regarded as limiting the present invention.

[0021] 1. Materials

[0022] The perennial ryegrass variety Yaqing was selected from Bailv International Grass Co., Ltd.

[0023] 2. Method

[0024] All the following operations were performed under sterile conditions.

[0025] 2.1 Callus induction and culture from mature ryegrass seed embryos

[0026] The method of callus induction and subculture is basically the same as the patent "Method for genetic transformation of perennial ryegrass (CN200510020540.7ZL)" that we have obtained. The specific operation is as follows:

[0027] ① Rinse the mature seeds of ryegrass with 70% (V / V) ethanol for 1 min, then soak in sodium hypochlorite solution with 2-4% active chlorine content, shake for 15 min, and then wash with sterile water 4-5 times;

[0028] ② Soak in sterile water at room t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of cell engineering, and specifically relates to a method for obtaining Lolium L. embryogenic callus and maintaining regeneration capacity of the callus. According to the present invention, the material for callus induction is the mature seed embryos of the Lolium L.; the mature embryos are adopted to induce the callus, after the mature embryos are cultured for 1 month, 4 secondary cultures are performed, wherein the time of the 4 secondary cultures is 2 months, and the secondary culture is performed every 2 weeks; a differentiation regeneration culture treatment is performed on the callus, the differentiated plant is continuously subjected to the secondary culture, and the callus incapable of differentiation is discarded; the stipe meristem area of the secondary culture plant after regenerating is subjected to the second callus induction, wherein the culture time is 1 month; the callus is subjected to the secondary culture for 2 months, wherein the culture media is changed every two weeks; then the differentiation regeneration culture treatment is performed on the callus, wherein the callus incapable of differentiation is discarded; a long-term secondary culture is performed on the regenerated plant, when the callus is required to carry out the relevant research, the stipe meristem area of the plant is subjected to induction and secondary culture to obtain the required callus, wherein the regenerated plant is subjected to the second differentiation. According to the present invention, the operation is simple and feasible, and the stable and high-frequency differentiation regeneration efficiency can be obtained.

Description

technical field [0001] The invention belongs to the technical field of cell engineering tissue culture, in particular to a method for obtaining ryegrass (Lolium L.) embryogenic callus and maintaining its regeneration ability, and related ryegrass callus induction, in vitro regeneration, etc. . Background technique [0002] Among the ryegrass plants, perennial ryegrass (Lolium perenne L.) and annual ryegrass (Lolium multiflorum L.) are widely cultivated. They are planted all over the world. They are introduced and cultivated in my country. They are excellent forage grass and lawn grass. , so it is of great significance to use transgenic technology to genetically improve it. [0003] The most important foundation of transgenic technology is tissue culture technology. At present, most of the genetic transformation of ryegrass is to transform its callus, and then screen and differentiate to obtain transgenic plants. Therefore, obtaining callus with differentiation ability is t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 马欣荣杨宏毛萍
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap