Efficient and fast method for separating mesenchymal stem cells

Abstract

The invention discloses a fast and efficient biological technical method for separating and amplifying umbilical cord mesenchymal stem cells. According to the method, umbilical cord tissues from full-term fetus born by cesarean section are utilized to obtain a large amount of mesenchymal stem cells through a mixed enzyme digestion method combined with an explant method, in a short period of time. This method effectively shortens enzyme digestion time, reduces loss of cell number to a largest degree, and improves the influence on biological activity of mesenchymal stem cells in a long enzyme digestion process. The method also optimizes in vitro amplification conditions; and the amplified cells have the biological characteristics of mesenchymal stem cells and high proliferation ability. The invention creates a fast and efficient biological technology for separation, purification and amplification of human umbilical cord mesenchymal stem cells, and provides an adequate seed cell source for research and clinical application of stem cells.

Description

technical field [0001] The invention is a biotechnology invention for extracting and cultivating umbilical cord mesenchymal stem cells rapidly and efficiently. The feature of the present invention is the joint application of enzyme digestion method and tissue adherence method, which optimizes the operation process of stem cell extraction, without the loss of stem cell quantity, and the mesenchymal stem cells after culture and expansion have the ability to differentiate into adipocytes and osteoblasts. item differentiation ability. Background technique [0002] Mesenchymal stem cells (MSCs) are pluripotent stem cells derived from mesoderm. MSCs widely exist in a variety of tissues, so far MSCs have been extracted from bone marrow, umbilical cord blood, adipose tissue, peripheral blood, skin and other tissues. [0003] MSCs have a powerful multi-directional differentiation function. Under suitable differentiation conditions, MSCs can differentiate into chondrocytes, adipocy...

AI Technical Summary

Problems solved by technology

However, prolonging the digestion time will have a greater negative impact on the proliferation activity and differentiation potential of cells.

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