Exopalaemon carinicauda compound eye development regulation gene and guide RNA, and acquisition and application thereof

Abstract

The invention relates to an exopalaemon carinicauda compound eye development regulation gene EcEy which can generate obvious phenotypic change in an embryo period after being successfully edited so asto indicate the success rate of gene editing operation, and the gene can also be applied to the establishment of gene editing platforms of other decapod crustaceans. The EcEy gene of the exopalaemoncarinicauda has a nucleotide sequence shown as SEQ ID No. 1 in a sequence list; the guide RNA (gRNA) designed and synthesized on the basis of the sequence has a ribonucleic acid sequence shown as SEQID No.2 in a sequence list; EcEy-gRNA and commercial Cas 9 mRNA are mixed and injected into fertilized eggs of exopalaemon carinicauda, a PAX structural domain of an EcEy gene can be specifically recognized and cut, and an obvious compound eye defect phenotype is observed in the early stage of embryonic development. The EcEy gene can be used as a target gene for gene editing research of exopalaemon carinicauda, is used for verifying whether gene editing operation succeeds or not, and can also be used for establishing gene editing platforms of other decades of crustaceans.

Description

technical field [0001] The invention relates to the technical field of genetic engineering of the white prawn, in particular to the acquisition of a compound eye development regulation gene and guide RNA of the white prawn and its application in gene editing research of decapod crustaceans. Background technique [0002] Exopalaemon carinicauda belongs to the crustacean subphylum Decapoda. It is a marine shrimp with high economic value. It is also one of the research models of decapod animals. It is the only one that has been reported Decapod animals capable of gene editing operations have the advantages of strong environmental adaptability, strong reproductive ability, easy artificial rearing, female shrimp can continuously lay eggs, and large and transparent embryos. The study of gene editing of the spinytail shrimp can be used to reveal the rules of life activities such as development, growth, metabolism and reproduction of decapod animals. However, the current research o...

AI Technical Summary

Problems solved by technology

However, the current gene editing research of the white shrimp has lacked effective marker genes, which should have obvious mutant phenotypes, and should be observed in the early stages of development, which can be used as a sign of the success of the gene editing operation.

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