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Method for constructing Exopalaemon carinicauda reciprocal backcross family

A technology of white shrimp and construction method, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of unstable germplasm source, unclear genetic background, restricting large-scale artificial breeding, etc., to avoid vertical diseases The effect of spreading, breeding and high survival rate, reducing mutual killing

Active Publication Date: 2017-02-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The white prawn has a good breeding prospect, but the current pond culture mainly relies on the natural broodstock broodstock in the fishing area to breed its own seedlings, which has the problems of unstable germplasm source and unclear genetic background, which seriously restricts the development of large-scale artificial breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, this example proposes a method for constructing a reciprocal backcross family of the white shrimp.

[0029] The construction method includes the following steps:

[0030] (1) Selection of broodstock: male and female prawns with mature glands, complete appendages, no mechanical damage, smooth body surface, and no Vibrio, ciliates, and white spot syndrome viruses through artificial breeding or natural collection. Individuals are used as broodstock, and the body weight of selected broodstock is ≥ 2.5g, and the body length is ≥ 50mm.

[0031] (2) Mating of broodstock: Male and female broodstock are sterilized and reared in separate breeding tanks according to the male to female ratio of 1:3. The body weight of the broodstock is 5%-10%, and eggs are laid by natural mating. The bait is lugworm, which is fed twice a day.

[0032] The individual disinfection method of male and female broodstock in the present embodiment is: place the broodstock in a medicated bath...

Embodiment 2

[0045] Utilize the construction method of the reciprocal backcross family of the white prawn of the present invention in Rizhao Haichen Aquaculture Co., Ltd. and Changyi Haifeng Aquaculture Co., Ltd. to carry out experimental research, but the method of the present invention is not limited to be applicable to these two place. After two years of exploratory experiments, small and pilot tests have proved that this method can successfully construct the reciprocal backcross family of the white shrimp, which can be used for the genetic analysis of quantitative traits of the white shrimp, the construction of near-isogenic lines and backcrossing. Breeding, etc. provide reliable experimental materials and basic data.

[0046] Take Rizhao Haichen Aquatic Products Co., Ltd. as an example: in 2014, Ningbo Xiangshan population was collected, and 16 groups of healthy white shrimp with complete appendages, smooth body surface and healthy body were selected as broodstock; the average weight ...

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Abstract

The invention provides a method for constructing an Exopalaemon carinicauda reciprocal backcross family. The method includes the following steps: (1) parent shrimp selection; (2) parent shrimp mating; (3) cultivation and breeding of F1 offspring seeds; (4) parent-child backcross; and (5) cultivation and breeding of backcross progeny offspring seeds. Male and female Exopalaemon carinicauda parent shrimps which are distributed naturally or bred artificially and have excellent characters are selected, and the reciprocal backcross family can be acquired through completely-artificial breeding, aquaculture and parent-child mating; the success rate of construction of the reciprocal backcross family can reach 75%, and can provide material and technique support for quantitative character genetic analysis, near-isogenic line construction and back cross breeding of the Exopalaemon carinicauda.

Description

technical field [0001] The invention belongs to the technical field of cultivation and cultivation of white shrimp artificial seed, and in particular relates to a method for constructing a reciprocal backcross family of white shrimp. Background technique [0002] Exopalaemon carinicauda (Exopalaemon carinicauda), also known as white shrimp, small white shrimp and winter jasmine, belongs to Arthropoda, Crustacea, Decapoda, Palaemonidae, white shrimp The genus (Exopalaemon) is mainly distributed in the shallow sea and low-salt waters along the entire mainland of China and the west coast of the Korean Peninsula. The Bohai Sea and the Yellow Sea have the highest production. It is one of the three unique economic shrimps in China. Its production is second only to Chinese shrimp (Fenneropenaeus chinensis) and Chinese hairy shrimp (Acetes chinensis). Due to its strong reproductive capacity, fast growth, wide environmental adaptability, and delicious meat, in recent years, with the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCY02A40/81
Inventor 李吉涛李健刘萍陈萍
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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