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Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof

An immune enhancer, the technology of Scutellaria baicalensis, applied in the field of preparation of cultured crustacean immune enhancer, can solve problems affecting the quality of cultured crabs and shrimps, economic losses of cultured swimming crabs, and drug residues in cultured crabs, etc., and achieve good disease prevention effect and good prevention and the effect of controlling

Inactive Publication Date: 2010-02-10
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diseases have also caused great economic losses to the cultured Portunus
At present, in terms of disease control, drugs are the main means of control, and the cost is high. The extensive use of antibacterial drugs has also led to a series of problems such as drug residues in cultured crabs and the emergence of drug-resistant strains, which greatly affects the quality of cultured crabs and shrimps.

Method used

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  • Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof
  • Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof
  • Scutellaria composite immune reinforcing agent, preparation method thereof and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Scutellaria baicalensis extract is extracted from Chinese herbal medicine fried scutellaria baicalensis. The specific preparation method is as follows:

[0030] ①Purchase fried Scutellaria baicalensis from Chinese medicine store, take 100g of fried Scutellaria baicalensis, crush it thoroughly, add 500mL of distilled water and soak overnight at 4℃;

[0031] ②Boil the soaked Scutellaria baicalensis for 30 minutes, replenish 250mL of water, boil for another 30 minutes, filter out the drug residue with gauze, and take the supernatant;

[0032] ③ Filter out the medicinal residues and add 250ml of water, decoct and filter out the medicinal residues as in ②, and then decoct twice;

[0033] ④Merge the medicinal liquid boiled three times, further decoct and concentrate to 100mL, filter to remove particles, and take the clarified filtrate;

[0034] ⑤Add 2mL 0.5M NaOH to the clarified filtrate, add 3 times the volume of 95% ethanol, and shake vigorously;

[0035] ⑥ After stan...

Embodiment 2

[0038] The preparation method of the Scutellaria baicalensis composite immune enhancer: take 2.5 parts of the dry product of the Scutellaria baicalensis prepared in Example 1 and 3 parts of levamisole, mix them thoroughly with a mixer, and store them in a cool and dark place.

[0039] Levamisole is veterinary levamisole hydrochloride (levamisole hydrochloride), purchased from domestic manufacturers, and its characteristics meet the requirements of the Chinese Veterinary Pharmacopoeia 2005 Edition (Part I).

Embodiment 3

[0041] In view of the fact that the main component of the extract of Scutellaria baicalensis is baicalin, Scutellaria baicalensis can also be used instead of the extract of Scutellaria baicalensis. The preparation method of the Scutellaria baicalensis composite immune enhancer: Take 2.5 parts of baicalin and 3 parts of levamisole, mix them thoroughly with a mixer, and store them in a cool and dark place.

[0042] Baicalin (Baicalin), also known as baicalin, is purchased from domestic manufacturers. The baicalin content of the product is not less than 95%, and the rest of the characteristics meet the requirements of the Chinese Pharmacopoeia 2005 edition (Part I).

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Abstract

The technology belongs to the technical field of aquiculture disease prevention, in particular to the preparation method of the immune reinforcing agent for cultivating crustacean, and the applicationmethod thereof. The aquiculture composite immune reinforcing agent comprises 2-4 parts of aquiculture extractive or baicalin and 2.5-5 parts of levamisole. The composite immune reinforcing agent caneffectively enhance non-specific immunity for cultivating blue crab, improve the activity of the blood serum phenol oxidase, the superoxide dismutase and the alkalinephosphatase of the blue crab, andimproves the disease resistance of the blue crab to common pathogens such as white spot syndrome virus, vibrio, hematodinium, etc. The composite immune reinforcing agent can be applied to the cultivation of the blue crab by being injected or added into blue crab fresh fish bait and granulated feed for preventing and controlling the disease at the disease peak and reducing the disease rate and themortality rate of the blue crab. The immune reinforcing agent is added into the feed for preventing and controlling the disease of the penaeus vannamei boone and exopalaemon carinicauda.

Description

technical field [0001] The technology belongs to the technical field of aquaculture disease prevention and control, and in particular relates to a preparation and application method of an immune enhancer for cultured crustaceans. technical background [0002] Seawater economic crabs are an important part of marine culture in my country. The main species of seawater crabs cultured in my country are Scylla serrata Forskal and Portunus sp, which belong to the order Crustacea and the order Decapoda. , Swimming crab family (Portunidae), widely distributed in the eastern coastal provinces of my country and Japan, India, Southeast Asia, Australia, South Africa and other waters, is an important marine economic crab in my country, and is also an important export-earning species. In recent years, my country's marine crab breeding industry has developed rapidly. In 2006, the breeding area of ​​mud crabs and swimming crabs in my country reached 762.77 million hectares, accounting for mor...

Claims

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Application Information

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IPC IPC(8): A61K36/539A61K31/7048A61P37/04A23K1/16A61K31/429A23K10/22A23K10/30A23K50/80
CPCY02A40/818
Inventor 钱冬刘问潘清清邱庆连
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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